35,000 to 100,000 nuclei were sorted into Buffer RLT and RNA was extracted using the standard AllPrep DNA/RNA Micro kit protocol (QIAGEN, Germantown, MD, USA). RNA was amplified using the Ovation RNA-Seq System V2 (NuGEN, San Carlos, CA, USA) according to the manufacturer’s protocol and resulting cDNA was quantified by Nanodrop 2000 (Thermo Fisher). cDNA was sheared using the Covaris S2 (Covaris, model S2, Woburn, MA, USA) to 200 base pairs and the resulting cDNA library was created using the Ovation Ultralow Library System kit (NuGEN) according to the manufacturer’s protocol. The size distribution of the library was assessed using BioAnalyzer Lab Chip 1000 (Agilent, Santa Clara, CA, USA), and quantified using the Qubit dsDNA kit (Invitrogen) and Kapa Biosystems Library Quantification kit (KapaBiosystems, Boston, MA). cDNA libraries were pooled, clustered on the cBot and sequenced using 100 or 125 base pairs paired end reads on the HiSeq 2000 or 2500 (Illumina, San Diego, CA, USA).
Allprep dna rna micro kit protocol
Manufactured by Qiagen
Sourced in United States
The AllPrep DNA/RNA Micro kit protocol is a laboratory equipment designed to extract and purify both DNA and RNA from small samples simultaneously. The protocol allows for the isolation of high-quality nucleic acids from a variety of sample types, including cells, tissues, and other biological materials.
Automatically generated - may contain errors
Lab products found in correlation
Nanodrop 2000, by Thermo Fisher Scientific (4 mentions)
Kapa biosystems library quantification kit, by Roche (2 mentions)
Ovation ultralow library system kit, by Tecan (2 mentions)
Bioanalyzer lab chip 1000, by Agilent Technologies (2 mentions)
Qubit dsdna kit, by Thermo Fisher Scientific (2 mentions)
Ovation rna seq system v2, by Tecan (2 mentions)
Hiseq 2000, by Illumina (2 mentions)
2 protocols using allprep dna rna micro kit protocol
1
Single-Cell RNA-Seq Library Preparation
2
Single-Cell RNA-Seq Library Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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35,000 to 100,000 nuclei were sorted into Buffer RLT and RNA was extracted using the standard AllPrep DNA/RNA Micro kit protocol (QIAGEN, Germantown, MD, USA). RNA was amplified using the Ovation RNA-Seq System V2 (NuGEN, San Carlos, CA, USA) according to the manufacturer’s protocol and resulting cDNA was quantified by Nanodrop 2000 (Thermo Fisher). cDNA was sheared using the Covaris S2 (Covaris, model S2, Woburn, MA, USA) to 200 base pairs and the resulting cDNA library was created using the Ovation Ultralow Library System kit (NuGEN) according to the manufacturer’s protocol. The size distribution of the library was assessed using BioAnalyzer Lab Chip 1000 (Agilent, Santa Clara, CA, USA), and quantified using the Qubit dsDNA kit (Invitrogen) and Kapa Biosystems Library Quantification kit (KapaBiosystems, Boston, MA). cDNA libraries were pooled, clustered on the cBot and sequenced using 100 or 125 base pairs paired end reads on the HiSeq 2000 or 2500 (Illumina, San Diego, CA, USA).
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