Arx 400 nmr spectrometer

All the solvents used in preparation and purification were of analytic grade. THF was distilled under normal pressure under nitrogen immediately prior to use. All the solvents used in the spectroscopic study were of HPLC grade and Milli-Q water was used as deionized water. All the chemicals were purchased from J&K Chemicals or Sigma-Aldrich and were used as received without further purification. The stock solutions of metal ions were prepared from KCl, CaCl₂, NaCl, MgCl₂·6H₂O, CuSO₄, MnCl₂, CoCl₂·6H₂O, Zn(NO₃)₂·7H₂O, NiCl₂·6H₂O, FeCl₂, FeCl₃, CdCl₂·2.5H₂O, AgNO₃, Pb(NO₃)₂ and HgCl₂ with doubly distilled water. ¹H and ¹³C NMR spectra were measured on a Bruker ARX 400 NMR spectrometer using CDCl₃ or CD₂Cl₂ as the solvent and tetramethylsilane (TMS) as an internal reference. UV absorption spectra were taken on a Milton Roy Spectronic 3000 array spectrophotometer. Photoluminescence (PL) spectra were recorded on a Perkin-Elmer LS 55 spectrofluorometer. Solid state quantum efficiency was measured using a Hamamatsu C11347 Quantaurus-QY integrating sphere at an excitation wavelength of 530 nm. High-resolution mass spectra (HRMS) were obtained on a GCT Premier CAB 048 mass spectrometer operated in MALDI-TOF mode. Particle sizes of the nano-aggregates were determined using a ZETA-Plus potential analyzer.

¹H and ¹³C NMR spectra were recorded on a Bruker ARX 400 NMR spectrometer. High‐resolution mass spectra (HRMS) were obtained on a Finnigan MAT TSQ 7000 Mass Spectrometer operating in a matrix‐assisted laser desorption/Ionization
time‐of‐flight (MALDI‐TOF) mode. Quantum yield was determined by a Quanta‐integrating sphere. ESR analysis was performed on a Bruker EMS^plus‐10/12 spectrometer. Absorption spectra were measured on a PerkinElmer Lambda 950 spectrophotometer. PL spectra were recorded on Edinburgh FS5 fluorescence spectrophotometer. Size distribution and zeta potential were analyzed on a DLS using a Malvern Zetasizer Nano ZSP. Particle size and morphology were observed on a HITACHI‐HT7700 transmission electron microscope. Laser confocal scanning microscope images were collected on a confocal laser scanning microscope (CLSM, ZEISS‐LSM880). The cell viability was detected by MTT assay, and the absorbance of each sample was measured at 570 nm using a microplate reader (BioTek). The cell internalization efficiency and apoptosis analysis were evaluated on a BD FACSAria SORP fluorescence activated cell sorting.

¹H and ¹³C NMR spectra were measured on a Bruker ARX 400 NMR spectrometer using chloroform-d as the deuterated solvent with tetramethylsilane (TMS; δ = 0) as the internal standard. Mass spectrum of non-alkylating TPE analogue was run by a Waters^R Micromass^R MALDI micro MX^TM Mass Spectrometer operating on the reflectron mode with DCTB (trans-2-[3-(4-tert-Butylphenyl)-2-methyl-2-propenylidene]malononitrile) as matrix. Mass spectrum of GSH-TPE-MI was recorded on an Agilent Technologies 6520 Accurate-Mass Q-TOF LC/MS operating in an ESI negative ion mode. Ultraviolet–visible (UV–Vis) absorption spectra were measured on Cary 50 UV–Vis spectrometer. Steady-state fluorescence signals were recorded on a Cary Eclipse fluorimeter.