Epoxides

N-methyl-1-phenylmethanamine, Et₃N, Pd/C, epoxides and other chemicals were purchased from Sigma-Aldrich. 1,2-distearoyl- sn-glycero-3-phosphocholine (DSPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), and 1-palmitoyl-2-oleoyl- sn-glycero-3-phosphoethanolamine (POPE) were purchased from Avanti Polar Lipids, Inc. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Amresco (Solon, OH). Buffered formaldehyde (10%, pH 7.4) was purchased from Ricca Chemical (Arlington, TX). Firefly luciferase mRNA (FLuc mRNA) was purchased from TriLink Biotechnologies, Inc. (San Diego, CA). Alexa fluor 488 conjugate of wheat germ agglutinin, NucBlue Fixed cell ready probes DAPI, ProLong diamond antifade mountant reagent, Ribogreen reagent and fetal bovine serum (FBS) were purchased from Life Technologies (Grand Island, NY). Alexa-Fluor 647-labeled RNA was purchased from Integrated DNA Technologies. Bright-Glo luciferase assay substrate was from Promega (Madison, WI, USA).

Amines, epoxides, acyl chlorides, TEA were purchased from Sigma Aldrich (Burlington, Massachusetts, USA), Tokyo Chemical Industry (TCI, Tokyo, Japan) and Ambeed (Arlington Heights, Illinois, USA). Alanine transaminase (ALT) colorimetric activity assay kit (#700260) and aspartate aminotransferase (AST) colorimetric activity assay kit (#701640) were purchased from Cayman Chemical (Ann Arbor, Michigan, USA). 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC), 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG 2000) and cholesterol were obtained from Avanti Polar Lipids (Alabaster, Alabama, USA). DLin-MC3-DMA and SM-102 were purchased from MedChem Express (Monmouth Junction, New Jersey, USA). Cas9 mRNA (5moU) was purchased from TriLink (San Diego, California, USA). Highly modified sgRNA target mouse TTR (guide No. G211) was chemically synthesized by AxoLabs (Kulmbach, Bayern, Germany) based on the previous publication^{43 (link)}. Anhydrous DCM, porcine liver esterase, amiloride hydrochloride, chlorpromazine, genistein, methyl-beta-cyclodextrin and TTR siRNAs (#NM_013697, siRNA IDs: SASI_Mm01_00076059, SASI_Mm01_00076060 and SASI_Mm01_00076061) were purchased from Sigma Aldrich (Burlington, Massachusetts, USA).