Anti–pThr-212/pSer-214/pThr-217 tau AT100 (MN1060) and anti-human tau HT7 (MN1000) were from Thermo Fisher; anti-GAPDH (MAB374) was from EMD Millipore; anti-Rab5 (ab18211), anti-p62 (ab91526), anti-NDP52 (ab68588), anti–galectin-8 (ab183637), and anti-ubiquitin (ab7780) were from Abcam; anti-p62 (ab610832) was from BD Biosciences; anti-LC3 (NB1000–2220) and anti-NDP52 (BO01P) were from Novus Biologicals; anti-optineurin (100000) was from Cayman; anti-FIP200 (17250–1-AP) was from Proteintech Group; anti-ubiquitin P4D1 (sc-8017) was from Santa Cruz Biotechnology; and anti–galectin-8 (AF1305) was from R&D Systems. Horseradish peroxidase (HRP)–conjugated secondary antibodies were from Bio-Rad. Alexa Fluor–conjugated secondary antibodies were from Life Sciences.
Anti ubiquitin ab7780
Manufactured by Abcam
Sourced in United States
Anti-ubiquitin (ab7780) is a research-use-only primary antibody that recognizes the ubiquitin protein. Ubiquitin is a small regulatory protein found in all eukaryotic cells and is involved in various cellular processes, such as protein degradation and signal transduction. This antibody can be used to detect and study ubiquitin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase hrp conjugated secondary antibody, by Bio-Rad (1 mentions)
Anti galectin 8 af1305, by R&D Systems (1 mentions)
Anti gapdh mab374, by Merck Group (1 mentions)
Anti fip200 17250 1 ap, by Proteintech (1 mentions)
Anti ubiquitin p4d1 sc 8017, by Santa Cruz Biotechnology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Cell Signaling Technology (1 mentions)
Cell lysis buffer, by Cell Signaling Technology (1 mentions)
Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions)
Anti ahr bml sa210, by Enzo Life Sciences (1 mentions)
Anti hif1 α 10006421, by Cayman Chemical (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Sb203580, by Merck Group (1 mentions)
Nocodazole, by Merck Group (1 mentions)
Ilomastat, by Merck Group (1 mentions)
U0126, by Merck Group (1 mentions)
Cytochalasin d cytod, by Merck Group (1 mentions)
Furin inhibitor 2, by Merck Group (1 mentions)
Electrophoresis reagents, by Bio-Rad (1 mentions)
4 protocols using anti ubiquitin ab7780
1
Tau and Autophagy Protein Antibodies
2
Immunoblot Analysis of Transcription Factors
3
Comprehensive Reagents for LRP-1 Study
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Electrophoresis reagents were purchased from Bio-Rad. HyGLO chemiluminescent HRP antibody detection reagents were from Denville Scientific Inc. Micro bicinchoninic acid protein assay reagents were from Pierce. The MMP inhibitor Ilomastat and the anti-LRP-1 light chain monoclonal antibody (mAb) (5A6) were purchased from EMD Millipore. Angiopep-2 and α2-macroglobulin were gifts from Angiochem Inc. The antibody against murine LRP heavy chain (8G1) was from Calbiochem, the anti-COX-2 antibody (610203) was from BD Biosciences, and the anti-glyceraldehyde 3-phosphate dehydhogenase (GAPDH) (Ab8245) and anti-ubiquitin (Ab7780) antibodies were from Abcam. The R-phycoerythrin (PE)-conjugated mouse antibodies against human CD91 and IgG1 κ isotype were from BD Biosciences. Horseradish peroxidase-conjugated donkey anti-rabbit and anti-mouse IgG secondary antibodies were from Jackson ImmunoResearch Laboratories. The anti-MT1-MMP hinge region antibody (M3927), ConA, cytochalasin-D (CytoD), nocodazole, furin inhibitor II, tofacitinib, SB203580, PP2, U0126, acetyl-11-keto-beta-boswellic acid, sodium dodecylsulfate (SDS), and bovine serum albumin (BSA) were from Sigma-Aldrich.
4
GAS Infection and Autophagy Markers
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Cells were seeded at 6 × 104 in 24-well plates with cover glass for overnight culture and infected with GAS for 30 min. Extracellular bacteria were killed by the use of 100 μg/ml gentamicin. At various time points postinfection, the cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, and stained with anti-LC3 (pM036; MBL), anti-Gal-3 (M3/38; Santa Cruz), anti-Gal-8 (D-18; Santa Cruz), anti-ubiquitin (ab7780; Abcam, Inc.), and anti-parkin (PRK8; Santa Cruz) antibodies at room temperature for 1 h. After the cells were washed with PBS, they were stained with Alexa Fluor-conjugated secondary antibodies and DAPI (4′,6-diamidino-2-phenylindole) for 1 h, and the samples were then analyzed by confocal microscopy (FV1000; Olympus).
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