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Multiplex suspension array

Manufactured by Bio-Rad
Sourced in Italy

The Multiplex suspension array is a versatile lab equipment designed for high-throughput, multiplexed analysis. It utilizes color-coded beads to allow simultaneous detection and quantification of multiple analytes in a single sample. The core function of the Multiplex suspension array is to enable efficient and accurate multi-analyte measurements, supporting a wide range of applications in life science research and diagnostics.

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2 protocols using multiplex suspension array

1

Multiplex Cytokine Profiling in Blood

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Plasma concentrations of IL-1β, IL-1ra, IL-6, bFGF, G-CSF, PDGF-bb, TNF-α and VEGF were measured using a customized multiplex suspension array (BioRad Laboratories, Milan, Italy) according to the manufacturer’s instructions. This assay allows the simultaneous detection of multiple molecules in a single well on 96-well microplate, using specific antibody-coupled fluorescently dyed beads in a sandwich immunoassay. White blood cells, ESR and CRP were determined on an automated modular Roche Cobas Analyzer.
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2

Multiplex Serum Analyte Quantification

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Serum levels of target analytes and were measured by multiplex suspension array purchased from Biorad, and read on a Bio-Plex 200 analyzer using Bio-Plex Manager Software using the manufacturer’s directions (Biorad, Hercules, CA). Individual serum samples were diluted 1:4 in sample diluent. A ten-point standard curve was used for each analyte and any high-end saturation points were removed from the standard curves for determining sample concentrations.
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