Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica, MA, USA) was used as per the instruction. Cell lysates and Ago2 antibody (1:50; MA5–14861; Invitrogen) conjugated on magnetic beads were co-cultured in RIP buffer overnight at 4 °C through rotation, while IgG antibody (1:500; 31,786; Invitrogen) was utilized as NC. Finally, the RNA precipitates were purified and extracted using the Imprint® RNA Immunoprecipitation Kit (RIP-12RXN, Sigma-Aldrich, USA) and RT-qPCR was then applied for relative RNA enrichment examination. The experiment was conducted for three times in an independent manner.
Imprint rna immunoprecipitation kit rip 12rxn
Manufactured by Merck Group
Sourced in United States
The Imprint® RNA Immunoprecipitation Kit (RIP-12RXN) is a tool for the study of RNA-protein interactions. It provides the necessary components to perform RNA immunoprecipitation experiments, allowing researchers to identify and analyze RNA molecules that associate with specific proteins of interest.
Automatically generated - may contain errors
3 protocols using imprint rna immunoprecipitation kit rip 12rxn
1
RNA-Binding Protein Immunoprecipitation
2
RIP Assay for METTL14 and HNRNPC Interactions
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With the application of Imprint® RNA Immunoprecipitation Kit (RIP-12RXN, Sigma-Aldrich, USA), RIP assay was implemented. PCa cells were collected and then lysed in RIPA lysis buffer (RIPA2-11-527, TBD, China). Subsequently, lysates were immunoprecipitated with anti-METTL14 (ab220030, Abcam), anti-HNRNPC (Abcam) or anti-IgG (Abcam). Finally, the RNA precipitates were extracted and then analyzed by RT-qPCR. The assay was independently implemented in triplicate.
3
AGO2-Mediated RIP Assay in LUSC
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With the Imprint® RNA Immunoprecipitation Kit (RIP-12RXN, Sigma-Aldrich, USA), RIP assay in H1703 and H226 cells was achieved with the specific antibodies and normal control anti-IgG antibody. Lysates were obtained from LUSC cell lines using RIP lysis buffer. The lysis was incubated with the magnetic beads conjugated with the AGO2 antibody or IgG antibody (ab6789, Abcam, UK). The precipitated RNAs were analyzed by RT-qPCR.
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