Antibiotics (Figure 1 ) enrofloxacin (ENRO), norfloxacin (NOR), lomefloxacin (LOME), enoxacin (ENOX), levoxacin (LEVO), ciprofloxacin (CIPRO), methacrylic acid (MAA), 2-trifluoromethacrylic acid (TFMAA) and 2-hydroxyethyl ethylene glycol dimethacrylate (EDMA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 2,2′-azobis(2,4-dimethylvaleronitrile) (ABDV) was purchased from Wako (Neuss, Germany) and used as received. Sarafloxacin hydrochloride (SARA), was a gift from Fort Dodge veterinaria (Girona, Spain). Danofloxacin (DANO) was purchased from Riedel-de-Haën (Seelze, Germany).
Acetonitrile (ACN) and methanol (MeOH) (HPLC-grade) were provided by SDS (Peypin, France) and trifluoroacetic acid (TFA) (HPLC-grade, 99%) was from Fluka (Buchs, Switzerland).
Water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). The monomers were purified, as required, by chromatography immediately before use, using an inhibitor–remover from Aldrich (Milwaukee, WI, USA). All solutions prepared for the HPLC were passed through a 0.45 µm nylon filter before use. 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) was supplied by Aldrich (Steinheim, Germany). Trifluoroacetic acid (TFA) (HPLC-grade) was from Fluka (Buchs, Switzerland).
Acetonitrile (ACN) and methanol (MeOH) (HPLC-grade) were provided by SDS (Peypin, France) and trifluoroacetic acid (TFA) (HPLC-grade, 99%) was from Fluka (Buchs, Switzerland).
Water was purified using a Milli-Q system (Millipore, Bedford, MA, USA). The monomers were purified, as required, by chromatography immediately before use, using an inhibitor–remover from Aldrich (Milwaukee, WI, USA). All solutions prepared for the HPLC were passed through a 0.45 µm nylon filter before use. 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) was supplied by Aldrich (Steinheim, Germany). Trifluoroacetic acid (TFA) (HPLC-grade) was from Fluka (Buchs, Switzerland).