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Sector imager 6000 plate reader

Manufactured by Mesoscale
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The SECTOR Imager 6000 is a high-performance plate reader designed for a variety of laboratory applications. It features a microplate imaging system that captures and analyzes data from multiple samples simultaneously. The SECTOR Imager 6000 supports a range of read modes, including absorbance, fluorescence, and luminescence, enabling researchers to conduct diverse assays and experiments.

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Lab products found in correlation

Msd mouse proinflammatory 5 plex plus kit, by Mesoscale (2 mentions) V plex plus kit, by Mesoscale (1 mentions) Ultra sensitive kits, by Mesoscale (1 mentions) Mouse tnf ri and tnfr 2 ultra sensitive kits, by Mesoscale (1 mentions) Micro bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Cholesterol quantitation kit, by Merck Group (1 mentions) Discovery workbench software, by Mesoscale (1 mentions) Lipocalin 2 ngal quantikine elisa kit, by R&D Systems (1 mentions) Mouse proinflammatory 7 plex ultra sensitive kit, by Mesoscale (1 mentions) Phosphatase inhibitor cocktail 1, by Merck Group (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions)

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SECTOR Imager 6000 (65 citations) SECTOR Imager (16 citations) SECTOR Imager 6000 reader (3 citations) SECTOR plates (2 citations)

5 protocols using sector imager 6000 plate reader

1

Quantifying Amyloid-beta and Cytokines

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Aβ1–40 and Aβ1–42 and cytokine concentrations in brain extracts and cell culture supernatants were determined with the MSD 96-Well MULTI-SPOT® Human (6E10) Abeta Triplex Assay (Meso Scale Discovery) or the V-PLEX™ Mouse Cytokine Assay according to manufacturer’s instructions and analyzed on a SECTOR Imager 6000 plate reader (Meso Scale Discovery).
Wagner L.K., Gilling K.E., Schormann E., Kloetzel P.M., Heppner F.L., Krüger E, & Prokop S. (2017). Immunoproteasome deficiency alters microglial cytokine response and improves cognitive deficits in Alzheimer’s disease-like APPPS1 mice. Acta Neuropathologica Communications, 5, 52.
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2

Cytokine and Chemokine Expression in Spinal Cord Mutant Mice

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Cytokine, chemokine, and TNF receptor expression in spinal cord lysates from LysMCreTnffl/fl and Tnffl/fl mice (naïve and 3 days survival) and Cx3cr1CreERTnffl/fl and Tnffl/fl mice (naïve and 3 h survival) were measured using the mouse Proinflammatory V-Plex Plus Kit (IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, CXCL1, TNF), mouse TNFRI, TNFRII, RANTES (CCL5) (LysMCreTnffl/fl and Tnffl/fl mice only) and MCP-1 (CCL2) (LysMCreTnffl/fl and Tnffl/fl mice only) Ultra-Sensitive Kits (Mesoscale Discovery) on a SECTOR Imager 6000 Plate Reader (Mesoscale Discovery) according to the manufacturer’s instructions. Samples were diluted two-fold prior to measurement according to manufacturer’s instructions. Samples were measured in duplicates and data was analyzed using MSD Discovery Workbench software.
Ellman D.G., Lund M.C., Nissen M., Nielsen P.S., Sørensen C., Lester E.B., Thougaard E., Jørgensen L.H., Nedospasov S.A., Andersen D.C., Stubbe J., Brambilla R., Degn M, & Lambertsen K.L. (2020). Conditional Ablation of Myeloid TNF Improves Functional Outcome and Decreases Lesion Size after Spinal Cord Injury in Mice. Cells, 9(11), 2407.
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3

Measuring Brain Cytokines and Receptors

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One series of brain tissue was lysed in Complete Mesoscale buffer according to the manufacturer’s protocol (Mesoscale Discovery, Rockville, MD, United States). Protein concentrations were determined according to the protocol from Micro BCA protein assay kit (Thermo Fischer Scientific). Cytokine and chemokine concentrations were estimated using an MSD Mouse Pro-Inflammatory V-Plex Plus Kit (Mesoscale Discovery) and TNF receptor concentrations using the Mouse TNF-RI and TNFR-II Ultra-Sensitive Kits (Mesoscale Discovery). XPro1595 levels were measured in ischemic brain tissue lysates using a human TNF V-Plex immunoassay (Mesoscale Discovery) as previously described (Karamita et al., 2017 (link)). The standard in the kit was replaced with XPro1595, which was diluted in the kit diluent number 2. Etanercept levels were measured using a human TNFRII Ultra-Sensitive immunoassay (Mesoscale Discovery) (Karamita et al., 2017 (link)). The standard in the kit was replaced with etanercept diluted in the kit diluent number 2. All kits were read on a SECTOR Imager 6000 plate reader (Mesoscale Discovery) according to the manufacturer’s instructions. All samples were run in duplex, and coefficient of variation (CV) values below 25% were accepted.
Yli-Karjanmaa M., Clausen B.H., Degn M., Novrup H.G., Ellman D.G., Toft-Jensen P., Szymkowski D.E., Stensballe A., Meyer M., Brambilla R, & Lambertsen K.L. (2019). Topical Administration of a Soluble TNF Inhibitor Reduces Infarct Volume After Focal Cerebral Ischemia in Mice. Frontiers in Neuroscience, 13, 781.
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4

Plasma biomarker analysis protocol

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The trunk blood was collected in EDTA and centrifuged at 2000g for 15 min at 4 °C. The plasma was collected and stored at − 80 °C until measurements for LCN2 (Lipocalin-2/NGAL Quantikine ELISA Kit, R&D Systems), IL-1, IL-6 (Mouse Proinflammatory 7-Plex Ultra-Sensitive Kit, Meso Scale Discovery immunoassays), triglycerides ELISA (Charles River Lab), total cholesterol (Cholesterol Quantitation Kit, Sigma-Aldrich), and insulin and leptin (Mouse Metabolic Kit (Multi-spot Assay System, Meso Scale Discovery). Plates were processed in a SECTOR® Imager 6000 plate reader (Meso Scale Diagnostics, LLC). Data acquired using the Discovery Workbench software (v4.0; Meso Scale Diagnostics, LLC).
De Sousa Rodrigues M.E., Houser M.C., Walker D.I., Jones D.P., Chang J., Barnum C.J, & Tansey M.G. (2019). Targeting soluble tumor necrosis factor as a potential intervention to lower risk for late-onset Alzheimer’s disease associated with obesity, metabolic syndrome, and type 2 diabetes. Alzheimer's Research & Therapy, 12, 1.
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5

Multiplex Cytokine Analysis in CNS Samples

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Multiplex analysis was performed in spinal cord tissues (thoracic segments), and in OPC and OL cultures. Spinal cord samples were obtained from the thoracic spinal cord of PBS-perfused mice homogenized in 300 μl RIPA buffer containing phosphatase inhibitor cocktail 1 (Sigma) and Complete protease inhibitor cocktail (Roche Diagnostics). OPC and OL samples were obtained by homogenizing cells obtained from 2 wells of a 6-well plate in 250 μl RIPA buffer with phosphatase and protease inhibitors. Cytokine expression was evaluated as previously described (Madsen et al., 2015 ) using the MSD Mouse Proinflammatory V-Plex Plus Kit, or individual plates for the assessment of CCL2, CCL5 and TNFR1 (Mesoscale). Samples were run on a SECTOR Imager 6000 Plate Reader (Mesoscale Discovery) and the data analyzed with the MSD Discovery Workbench software. Samples with CV values above 25% were excluded.
Madsen P.M., Desu H.L., Vaccari J.P., Florimon Y., Ellman D.G., Keane R.W., Clausen B.H., Lambertsen K.L, & Brambilla R. (2019). Oligodendrocytes modulate the immune-inflammatory response in EAE via TNFR2 signaling. Brain, behavior, and immunity, 84, 132-146.
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