Antibody against atr

Manufactured by Fortis Life Sciences

The Antibody against ATR is a laboratory reagent used to detect and study the presence and expression levels of the ATR (Ataxia Telangiectasia and Rad3-related) protein in various biological samples. ATR is a serine/threonine-protein kinase that plays a critical role in the cellular response to DNA damage and replication stress. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to aid in the investigation of ATR-mediated signaling pathways and cellular processes.

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Lab products found in correlation

Antibodies against phospho chk1 s345, by Apexbio (2 mentions) Phospho atr antibody t1989, by GeneTex (2 mentions) Ve 822, by Apexbio (2 mentions) Hmgb1 antibody, by GeneTex (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions)

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ATR antibody (2 citations)

2 protocols using antibody against atr

Analyzing ATR and DNA Damage Response Pathways

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Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS and 1% penicillin/streptomycin. The ATR inhibitor VE-822 (cat# B1383) was from ApexBio Technology. Antibodies against phospho-Chk1 (S345; cat# 2348), Chk1 (cat# 2360), actin (Cat# 4970), PARP1 (cat# 9542), cleaved caspases 3 (cat# 9661) and 7 (cat# 9491), γ-H2AX (A300–081A), ATM (cat# A300–299A), and GAPDH (cat# 2118) were from Cell Signaling Technology. The antibody against ATR (cat# A300–137A) was from Bethyl Laboratories. The phospho-ATR antibody (T1989; cat# GTX128145) and HMGB1 antibody (cat# GTX101277) were from GeneTex. Antibody against poly (ADP-ribose) (PAR) (Cat#4338-MC-50) was from Trevigin.

Li Z., Wang‐Heaton H., Cartwright B.M., Makinwa Y., Hilton B.A., Musich P.R., Shkriabai N., Kvaratskhelia M., Guan S., Chen Q., Yu X, & Zou Y. (2021). ATR prevents Ca2+ overload‐induced necrotic cell death through phosphorylation‐mediated inactivation of PARP1 without DNA damage signaling. The FASEB Journal, 35(5), e21373.

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ATR Signaling Pathway Regulation in Cell Culture

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Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS and 1% penicillin/streptomycin. The ATR inhibitor VE‐822 (cat# B1383) was from ApexBio Technology. Antibodies against phospho‐Chk1 (S345; cat# 2348), Chk1 (cat# 2360), actin (Cat# 4970), PARP1 (cat# 9542), cleaved caspases 3 (cat# 9661) and 7 (cat# 9491), γ‐H2AX (A300‐081A), ATM (cat# A300‐299A), and GAPDH (cat# 2118) were from Cell Signaling Technology. The antibody against ATR (cat# A300‐137A) was from Bethyl Laboratories. The phospho‐ATR antibody (T1989; cat# GTX128145) and HMGB1 antibody (cat# GTX101277) were from GeneTex. Antibody against poly (ADP‐ribose) (PAR) (Cat#4338‐MC‐50) was from Trevigin.

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