Linezolid

Linezolid was purchased from LKT Laboratories (St. Paul, MN); DSPE-mPEG (1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000]), cholesterol and DPPE-lissamine rhodamine B (1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) from Avanti Polar Lipids (Alabaster, AL); ), lecithin (from soybean), gentamicin from Sigma-Aldrich (St. Louis, MO); PLGA (poly(D,L-lactic-co-glycolic acid), 50:50 monomer ratio, ester cap, MW 15kDa) from Polyscitech (West Lafayette, IN). Tryptic soy broth (TSB) and agar were supplied by Becton-Dickinson (Cockeysville, MD). Wheat germ agglutinin-Alexa Fluor 633 conjugate for cell plasma membrane labeling and DAPI (4’,6-diamidino-2-phenylindole) as nuclear dye, Fluoromount aqueous mounting medium, HEPES (N-(2-hydroxyethyl)piperazine-N’-2-ethanesulfonic acid) and most solvents used were bought from Thermo-Fisher Scientific (Pittsburg, PA, USA). Triton X-100 was purchased from Bio-Rad (Hercules, CA).

Clinical isolates of MSSA, MRSA-USA100, MRSA-USA300, VISA, VRSA, and LRSA were provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by the Biodefense and Emerging Infections Research Resources Repository (BEI Resources), NIAID, NIH. The BEI Resources Web site (https://www.beiresources.org ) describes the location of origin of each isolate. Luria−Bertani (LB) media was obtained from Millipore. Cation-adjusted Mueller Hinton (CAMH) media and tryptic soy agar (TSA) were obtained from Becton Dickson. Phosphate-buffered saline (PBS) was obtained from BioWhittaker and dimethyl sulfoxide (DMSO) was obtained from Fluka Analytical. High-resolution agarose, acetonitrile, trifluoroacetic acid (TFA), mouse serum, porcine mucin, neostigmine bromide, and vancomycin hydrochloride were obtained from Sigma. Linezolid was obtained from LKT Laboratories. TXA707, TXA709, and BOFP were synthesized as described previously.^{26 (link),36 (link)}