Inova 500m

The morphological structures of Ag@MOF nanoparticles were imaged by Transmission electron micrograph (TEM; H800, Hitachi, Japan) at 200 kV. The elemental distributions of Ag@MOF were measured by energy‐dispersive x‐ray spectroscopy (EDS). The size distributions of Ag@MOF were detected and then precisely analyzed by dynamic light scattering (DLS; Zeta‐Sizer Nano‐ZS, Malvern, UK). The chemical structure of Gel, GelMA, HA, and HA‐E was characterized by ¹H NMR (Inova‐500M, Varian, America). Fourier‐transform infrared spectroscopy (FTIR; Vertex‐70, Bruker, Germany) was utilized to determine the functional groups of Gel, GelMA, HA, and HA‐E. Scanning electron microscope (SEM; S‐3400, Hitachi, Japan) was used to obtain and analyze images of microscopic morphology of the composite hydrogels with an acceleration potential of 5 kV. Finally, the average pore size of the synthesized material was calculated by Nano measure software.

Well‐defined modified CS‐FPBA was prepared via the Schiff base reaction between amino groups and aldehyde groups. A coupling reaction between the aldehyde groups of FPBA and the amino group of CS was performed. Briefly, CS (2 g, ≈0.01 mmol CS) was dispersed in an acetic acid solution (1%, 200 mL). After 4 h of activation, FPBA (0.02 mmol) was slowly added to the mixed solution and stirred 12 h at room temperature, while a pH of 5.0 was maintained by the use of 1 m HCl. Unreacted FPBA was removed by a dialysis membrane tube with a molecular weight cutoff of 3.5 kDa in PBS (pH 5.0) for 48 h followed by dialysis against distilled water for 3 d. CS‐FPBA was obtained after lyophilization and was stored at 4 °C until use. CS was dispersed in an acetic acid solution, and CS‐FPBA were dispersed in distilled water, and their UV–vis spectra were measured by a WFZ‐26A UV–vis spectrophotometer (Tianjin Automatic Science Instrument Plant, China). To confirm conjugation, Fourier transform infrared spectroscopy (FT‐IR) was carried out using KBr disks by on a BIO‐RAD FT‐IR 3000 (BIO‐RAD Company, Hercules, USA), and the molar fractional ratio of conjugated carboxylic acid groups was calculated using the 1H NMR spectra of the products recorded by a Varian Inova‐500 M instrument (Varian Inc., Palo Alto, USA) with D2O as a solvent.

The chemical structure of GelMA and OP3‐MA was characterized by Fourier transform infrared spectrometer (FTIR, Inova‐500M, Varian, USA) and nuclear magnetic resonance spectrometer (NMR, VERTEX 70, Burke, Germany). For FTIR characterization, 5 mg of samples was first mixed with 30 mg of potassium bromide (KBr) and ground into powder, then the powder was pressed into a transparent flake and tested by FTIR. The scan range of the wavenumber was 4000–500 cm⁻¹, and the resolution was 4 cm⁻¹. As for NMR characterization, samples were dissolved in deuterated water (D₂O), and the hydrogen spectrum was detected.