Hat medium supplement

Freund’s complete and incomplete adjuvants, bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), HAT medium supplement and HT medium supplement were purchased from Sigma Aldrich (USA). Protein G resin and the peroxidase conjugated goat antimouse IgG (IgG-HRP) were purchased from TransGen Biotech (China). Other reagents were of chemical grade from Beijing Solarbio Science & Technology Co. Ltd. (China). Female Balb/c mice were purchased from the Animal Research Institute of Nanchang University (China). Animal manipulation was performed in compliance with institutional guidelines approved by the Nanchang University (Nanchang, China).

Brevetoxins were purchased from Taiwan Algal Science Inc. (Algal Science Inc., Taiwan, China). Okadaic acid (OA), conopeptide (CTX), polyethylene glycol 1450 (PEG 1450), tetrodotoxin (TTX), HAT medium supplement, HT medium supplement, Mouse monoclonal Antibody Subtyping Kit, tetramethyl benzidine (TMB), Horseradish peroxidase (HRP) conjugated goat anti-mouse IgG, and RPMI 1640 were purchased from Sigma-Aldrich Co. Ltd. (St. Louis, MO, USA). Other reagents were chemical grade from Sinopharm Chemical Regent Co. Ltd. (Beijing, China). Female BALB/c mice were purchased from Wushi Animal Laboratory (Shanghai, China). Myeloma cells were stored in our lab. All animal studies and the procedures used in this study were approved by the Research Ethics Committee, the Fujian Key Laboratory of Pathogenic Fungi Mycotoxins, Fujian Agriculture and Forestry University, Fujian, China (Permit No. PFMFAFU201610). Approval date was 1 November 2016.