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Taqman fast one step qpcr master mix

Manufactured by Thermo Fisher Scientific
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The TaqMan Fast One-Step qPCR Master Mix is a reagent used for real-time quantitative PCR (qPCR) analysis. It is designed to perform one-step RT-qPCR, combining the reverse transcription and PCR amplification steps in a single reaction mixture.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Steponeplus qrt pcr system, by Thermo Fisher Scientific (2 mentions) Taqman probes for genes of interest, by Thermo Fisher Scientific (2 mentions) House keeping gene probes, by Thermo Fisher Scientific (2 mentions) Nandrop 2000, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

TaqMan Master Mix (448 citations) TaqMan qPCR (43 citations) QPCR Master Mix (38 citations) Fast Taqman mix (4 citations) TaqMan qPCR Mix (4 citations)

2 protocols using taqman fast one step qpcr master mix

1

Gene Expression Analysis of Kidney Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA extraction from kidney organoids following drug exposure was performed using an RNeasy Mini kit (Qiagen, Germany) per manufacturer’s instructions. RNA was quantified with spectrophotometry with a NanDrop 2000 (Thermo Fisher, Carlsbad, CA). To analyse gene expression, TaqMan Fast One-Step qPCR Master Mix (Applied Biosystems, Foster City, CA), TaqMan Probes for genes of interest (ThermoFisher, Carlsbad, CA), and house-keeping gene probes (Applied Biosystems, Foster City, CA) were combined in assigned wells with RNA. All qPCR reactions were performed and analysed on a StepOnePlus qRT-PCR system (Applied Biosystems, Foster City, CA). All data was normalized to house-keeping gene GAPDH prior to normalizing to control samples.
Lawlor K.T., Vanslambrouck J.M., Higgins J.W., Chambon A., Bishard K., Arndt D., Er P.X., Wilson S.B., Howden S.E., Tan K.S., Li F., Hale L.J., Shepherd B., Pentoney S., Presnell S.C., Chen A.E, & Little M.H. (2020). Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation.. Nature materials, 20(2), 260-271.
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2

Gene Expression Analysis of Kidney Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA extraction from kidney organoids following drug exposure was performed using an RNeasy Mini kit (Qiagen, Germany) per manufacturer’s instructions. RNA was quantified with spectrophotometry with a NanDrop 2000 (Thermo Fisher, Carlsbad, CA). To analyse gene expression, TaqMan Fast One-Step qPCR Master Mix (Applied Biosystems, Foster City, CA), TaqMan Probes for genes of interest (ThermoFisher, Carlsbad, CA), and house-keeping gene probes (Applied Biosystems, Foster City, CA) were combined in assigned wells with RNA. All qPCR reactions were performed and analysed on a StepOnePlus qRT-PCR system (Applied Biosystems, Foster City, CA). All data was normalized to house-keeping gene GAPDH prior to normalizing to control samples.
Lawlor K.T., Vanslambrouck J.M., Higgins J.W., Chambon A., Bishard K., Arndt D., Er P.X., Wilson S.B., Howden S.E., Tan K.S., Li F., Hale L.J., Shepherd B., Pentoney S., Presnell S.C., Chen A.E, & Little M.H. (2020). Cellular extrusion bioprinting improves kidney organoid reproducibility and conformation.. Nature materials, 20(2), 260-271.
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