Dulbecco′s modified Eagle׳s medium, fetal bovine serum, Trypsin, Lipofectamine 2000 and TRIzol reagent were all purchased from Invitrogen (Carlsbad, CA). RevertAidTM Reverse Transcriptase was obtained from MBI Fermentas (Hanover, MD). Dual-Luciferase® Reporter Assay Kit was ordered from Promega (Madison, WI). Plasmid miniprep kit was purchased from Qiagen (Hilden, Germany). Oligonucleotide primers were synthesized by Sangon Co. (Shanghai, China). Antibodies of rabbit anti-human CYP3A4 were provided by AVIVA Systems Biology (California, U.S.A.). Alexa Fluor 488 F[ab’]2 of goat anti-rabbit IgG [H+L] was from MultiSciences Biotech Co., Ltd. (Hangzhou, China), and horseradish peroxidase-labeled goat anti-rabbit IgG was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Rifampin was purchased from MP Biomedicals Inc. (Eschwege, Germany).
Rifampin
Manufactured by MP Biomedicals
Sourced in United States
Rifampin is a chemical compound used in laboratory settings. It functions as an antibiotic agent, inhibiting the growth and reproduction of certain types of bacteria.
Automatically generated - may contain errors
Lab products found in correlation
Kanamycin, by Avantor (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Revertaidtm reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Plasmid miniprep kit, by Qiagen (1 mentions)
Horseradish peroxidase labeled goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Dual luciferase reporter assay kit, by Promega (1 mentions)
Oligonucleotide primers, by Sangon (1 mentions)
Kanamycin, by Transgene (1 mentions)
Trans1 t1 phage resistant chemically competent cell, by Transgene (1 mentions)
Kanamycin, by Tiangen Biotech (1 mentions)
Luria bertani medium, by Thermo Fisher Scientific (1 mentions)
Gentamicin, by AppliChem (1 mentions)
Ciprofloxacin, by MP Biomedicals (1 mentions)
4 protocols using rifampin
1
Luciferase Assay Protocol for CYP3A4 Regulation
2
Genetic Modification of P. ostreatus
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The P. ostreatus CCMSSC 00389 strain was provided by the China Center for Mushroom Spawn Standards and Control. The WT, OE-aox, and RNAi-aox strains were incubated on PDA plates. Agrobacterium tumefaciens GV3101 (IMCAS, Beijing, China) was grown in Luria-Bertani (LB) medium (Oxoid, England) containing 100 µg/mL kanamycin (VWR Life Science, USA) and 50 µg/mL rifampin (MP Biomedicals, France) and was used to transform P. ostreatus. Trans 1-T1 phage-resistant chemically competent cells (TransGen Biotech, Beijing, China) were used for plasmid construction and were grown in LB broth containing kanamycin (50 µg/mL).
3
Genetic Manipulation of Pleurotus ostreatus
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P. ostreatus P89 (CCMSSC 00389) was provided by the China Center for Mushroom Spawn Standards and Control. The WT, OE-aco, and RNAi-aco strains were cultured on PDA plates for growth tests and recovery growth tests after heat stress. Agrobacterium tumefaciens GV3101 (IMCAS, Beijing, China) was grown in Luria-Bertani (LB) medium (Oxoid, England) containing 100 μg/ml kanamycin (VWR Life Science, USA) and 50 μg/ml rifampin (MP Biomedicals, France) and was used to transform P. ostreatus. Escherichia coli DH5α and BL21(DE3) (Tiangen, Beijing, China) were used for plasmid construction and were grown in LB broth containing kanamycin (50 μg/ml).
4
Antibiotic Pharmacodynamics Comparative Analysis
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Antibiotics were purchased from Sigma (gentamicin (GEN), oxacillin (OXA), vancomycin (VAN), and tetracycline (TET)), AppliChem (ciprofloxacin (CIP), rifampin (RIF)), TCI (daptomycin (DAP)), MP Biochemicals (erythromycin (ERM), and Chem-Impex International (linezolid (LIN)). The minimum inhibitory concentration (MIC) for each drug was determined by the standard two-fold dilution protocol [39 (link)]. In the experiments, each antibiotic was used at 2×MIC and 10×MIC. We chose these antibiotics to have a broad range of pharmacodynamic properties. The first eight antibiotics listed above are grouped by their pharmacodynamic characteristics: in classic assays of killing dynamics in liquid cultures at super MIC concentrations, CIP, DAP and GEN kill rapidly (fast-acting bactericidal antibiotics); OXA and VAN kill more slowly (slow-acting antibiotics); and LIN, ERM and TET prevent growth (bacteriostatic antibiotics) [40 (link)].
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