The examinations of circulating hormones were determined by each physician for evaluating various endocrine disorders. Blood collections were carefully performed in a relaxed sitting position around late morning to early afternoon time in the same way when the patients visited our clinic. The auto-analyzer system Cobas 8000 (F. Hoffmann-La Roche AG, Basel, Switzerland) was used for measuring hormone levels at the Central Laboratory of our facility. As reported previously [12 (link)], assays for plasma adrenocorticotropin (ACTH) and serum cortisol were performed by an electro-chemiluminescence immunoassay (ECLIA) using Elecsys ACTH and Elecsys Cortisol II kits (F. Hoffmann-La Roche AG), respectively. The assays for serum free-thyroxin (FT4) and thyrotropin (TSH) were performed by Elecsys FT4 III and TSH kits, and the assays for serum GH and IGF-I were completed by Elecsys GH and IGF-I kits (F. Hoffmann-La Roche AG), respectively. Serum levels of IGF-I were then calculated to the values of standard deviation (SD) [17 (link)].
Elecsys acth
Manufactured by Roche
Sourced in Switzerland
The Elecsys ACTH is an in vitro diagnostic test used to measure the adrenocorticotropic hormone (ACTH) levels in human serum and plasma. ACTH is a hormone produced by the pituitary gland that plays a crucial role in the regulation of the body's stress response and cortisol production. The Elecsys ACTH assay provides a quantitative determination of ACTH concentration, which can be used to aid in the diagnosis and monitoring of various endocrine disorders.
Automatically generated - may contain errors
Lab products found in correlation
Elecsys cortisol 2 kits, by Roche (2 mentions)
Elecsys ft4 3 and tsh kits, by Roche (2 mentions)
Elecsys gh and igf 1 kits, by Roche (2 mentions)
Cobas 8000, by Roche (2 mentions)
Elecsys cortisol 2, by Roche (2 mentions)
Elecsys gh, by Roche (1 mentions)
Elecsys igf 1 kits, by Roche (1 mentions)
Elecsys ft4iii, by Roche (1 mentions)
Access dhea s, by Beckman Coulter (1 mentions)
4 protocols using elecsys acth
1
Standardized Endocrine Hormone Analysis
2
Comprehensive Endocrine Biomarker Profiling
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Blood sampling was performed in a relaxed sitting position at the time each patient visited the CAC, which was around late morning to early afternoon. The blood tests were determined by each physician, and the measurements were performed using the Cobas 8000 auto-analyzer system (F. Hoffmann-La Roche AG, Basel, Switzerland) at the central laboratory of our facility. Assays for serum ferritin were performed by an electro-chemiluminescence immunoassay (ECLIA) using the Elecsys Ferritin kit (F. Hoffmann-La Roche AG). Assays for plasma adrenocorticotropin (ACTH) and serum cortisol were also performed by ECLIA using Elecsys ACTH and Elecsys Cortisol II kits (F. Hoffmann-La Roche AG), respectively, as previously reported [8 (link)]. Assays for serum free thyroxin (FT4) and thyrotropin (TSH) were performed using Elecsys FT4 III and TSH kits, and assays for serum growth hormone (GH) and insulin-like growth factor (IGF)-I were performed using Elecsys GH and IGF-I kits (F. Hoffmann-La Roche AG), respectively.
3
Endocrine Disorder Evaluation Protocol
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The decision to examine serum and plasma hormones for evaluation of the possibility of some endocrine disorders was made by each physician. Blood samples were collected in a sitting position at the time when the patients visited our clinic at late morning time. Information on the following biochemical parameters was also obtained: ACTH, cortisol, growth hormone (GH), insulin-like growth factor (IGF)-I, free thyroxin (FT4), and thyrotropin (TSH). The levels of those parameters were determined by using the auto-analyzer system Cobas 8000 (F. Hoffmann-La Roche AG, Basel, Switzerland) at the Central Laboratory of Okayama University Hospital. Plasma ACTH and serum cortisol were measured by an electro-chemiluminescence immunoassay (ECLIA) method using Elecsys ACTH and Elecsys Cortisol II kits (F. Hoffmann-La Roche AG), respectively. Serum GH and IGF-I were measured using Elecsys GH and Elecsys IGF-I kits (F. Hoffmann-La Roche AG), respectively, and IGF-I levels were shown by the standard deviation (SD) values [18] . Serum FT4 and TSH were determined by Elecsys FT4 III and Elecsys TSH kits (F. Hoffmann-La Roche AG), respectively.
4
Rapid ACTH Stimulation Test for HPA Axis Assessment
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Serum DHEA-S levels were measured using a chemiluminescence enzyme immunoassay kit (Access DHEA-S, Beckman Coulter, Inc. Brea, CA, USA). Cortisol and ACTH levels were measured using electrochemiluminescence immunoassay kits (Elecsys Cortisol II and Elecsys ACTH, Roche Diagnostics K. K. Basel, Switzerland). Serum DHEA-S measurement and the rapid ACTH stimulation test were performed on the same day or, if not possible, no more than 7 days apart. The rapid ACTH test was performed by intravenous administration of 250 μg synthetic ACTH (1-24) (Cortrosyn, Daiichi Pharmaceutical Co., Tokyo, Japan). The serum cortisol levels were measured in blood samples collected before and 30 and 60 minutes after the administration. A cut-off value of 18 μg/dL (500 nmol/L) for peak serum cortisol levels was used for HPA insufficiency diagnosis [2] . The 65 patients were divided into two groups according to the peak cortisol level: the impaired HPA axis group (those with a peak cortisol level <18 μg/dL (n = 37)) and the intact HPA axis group (those with a peak cortisol level ≥18 μg/dL (n = 28)).
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