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Cd48 fitc

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CD48-FITC is a fluorescently labeled monoclonal antibody that binds to the CD48 cell surface antigen. CD48 is a glycosylphosphatidylinositol (GPI)-linked cell surface protein that functions in cell-cell adhesion and activation signaling.

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Lab products found in correlation

Sca 1 pe cy7, by BioLegend (4 mentions) Cd150 percp cy5, by BioLegend (2 mentions) C kit apc, by BioLegend (2 mentions) Cd34 fitc ram34, by Thermo Fisher Scientific (2 mentions) Cd150 apc, by BioLegend (2 mentions) Cd117 apc cy7, by BioLegend (2 mentions) Flk2 pe, by BioLegend (2 mentions) Mac 1 pe cy5, by BioLegend (2 mentions) Cd45.2 fitc 104, by BioLegend (2 mentions) Cd45.1 apc cy7 a20, by BioLegend (2 mentions) Cd16 32 percp cy5.5 93, by BioLegend (2 mentions) Cd5 apc cy7, by BioLegend (2 mentions) Cd19 pe ebio1d3, by Thermo Fisher Scientific (2 mentions) Sytox blue viability stain, by Thermo Fisher Scientific (2 mentions) B220 pe cy7, by BioLegend (2 mentions) Cd3 pe, by BioLegend (2 mentions) Annexin 5 apc, by BioLegend (2 mentions) Lsrfortessa, by BD (2 mentions) Ter119 apc cy7, by BioLegend (2 mentions) Facsaria, by BD (1 mentions)

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Anti-CD48 FITC (2 citations)

5 protocols using cd48 fitc

1

Isolation and Phenotyping of Murine and Human Hematopoietic Cells

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Cells were stained with flurochrome-conjugated antibodies at 4 °C for 30 min and washed with cold PBS. Cell sorting and phenotypic analysis were done using a FACSAria or an LSRII or FACSCFortessa, respectively (Becton Dickenson, San Diego, CA). OBs were isolated as described above using CD45-FITC (BD, 553080), CD31-FITC (Pharmingen, PM-01954D), Ter119-FITC (BioLegend, 116206) and Sca1-PE/Cy7 (BioLegend, 122514). For mouse BM HSC sorting and phenotyping analysis, the following antibodies were used: Lineage-Pacific Blue (BioLegend, 133306), Sca1-PE/Cy7 (BioLegend, 122514), c-Kit-APC (BioLegend, 105811), CD48-FITC (Biolegend, 103403), CD150-PerCP/Cy5.5 (BioLegend, 115921). Antibodies used for human CB cell sorting were CD34-APC (BD, 555824) and CD166-PE (BD, 559263).
Zhang J., Ghosh J., Mohamad S.F., Zhang C., Huang X., Capitano M.L., Gunawan A.M., Cooper S., Guo B., Cai Q., Broxmeyer H.E, & Srour E.F. (2019). CD166 engagement augments mouse and human hematopoietic progenitor function via activation of stemness and cell cycle pathways. Stem cells (Dayton, Ohio), 37(10), 1319-1330.
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2

Multiparametric Immunophenotyping of Hematopoietic Cells

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Anti- mouse Ter119-BV421, Sca-1-APC, CD34-FITC, CD135-PE, IL-7Ra-BV605, CD48-FITC, CD150-PE Cy7, CD45.2-Alexa Fluor 700/APC Cy7, CD11b-BV605, CD3-APC, NK1.1-APC Cy7, CD19-BV605, IgM-Alexa Fluor 647, CD43-PE Cy7, CD16/32-PerCP Cy5.5, CD16/32; anti-human CD34-APC Cy7 (clone: 581), CD38-PE Cy7 (HB-7), CD45RA-BV 605 (HI100), CD90-APC (5E10), CD49f-PerCP Cy5.5 (GoH3), CD45-PE/Dazzle 594 (HI30), and CD33-APC (P67.6) antibodies; and Legendplex™ Mouse Inflammation Panel (13-plex) and Mouse IL-6 ELISA were purchased from Biolegend (San Diego, CA). IL6 depleting AB (clone MP5–20F3) from BioXCell (West Lebanon, NH).
Anti-mouse c-Kit-PE Cy7, Mouse Hematopoietic Lineage Biotin Panel, and anti-human CD19-Alexa Fluor 700 (HIB19) from eBioscience (San Diego, CA). Anti-B220-PE CF594 from BD biosciences (San Jose, CA). EasySep Human Progenitor Cell Enrichment Kit from Stem Cell Technologies Inc. (Vancouver, BC). Anti-human EPCR-FITC (RCR-49) from Santa Cruz Biotechnology®, inc. (Dallas, TX). Anti-human CD141-PE (AD5–14H12) from Miltenyi Biotec Inc. (Auburn, CA).
Basu S., Liang H.P., Hernandez I., Zogg M., Fields B., May J., Ogoti Y., Wyseure T., Mosnier L.O., Burns R.T., Carlson K, & Weiler H. (2019). Role of Thrombomodulin expression on hematopoietic stem cells. Journal of thrombosis and haemostasis : JTH, 18(1), 123-135.
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3

Comprehensive Immune Cell Profiling

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The following antibodies were used (all from BioLegend): CD3 (clone 17A2), Ter119 (TER-119), Gr-1 (RB6–8C5), B220 (RA3–6B2) conjugated to Pacific Blue, CD117 APC-Cy7 (2B8), Sca-1 PE-Cy7 (D7), Flk2 PE (A2F10), CD48 FITC (HM48–1), CD150 APC (TC15–12F12.2), B220 PE-Cy7 (RA3–6B2), Mac-1 PE-Cy5 (M1/70), CD3 PE (17A2), Ter119 APC-Cy7 (TER-119), CD45.2 FITC (104), CD45.1 APC-Cy7 (A20), CD16/32 PerCP-Cy5.5 (93), CD5 APC-Cy7 (53–7.3). CD19 PE (eBio1D3) and CD34 FITC (RAM34) were from eBioscience. Cells were labeled with SYTOX Blue viability stain (Thermo). Data were acquired on LSRII or LSR Fortessa instruments (BD Biosciences). APC-annexin V was from BioLegend.
Wang D., Tanaka-Yano M., Meader E., Kinney M.A., Morris V., Lummertz da Rocha E., Liu N., Liu T., Zhu Q., Orkin S.H., North T.E., Daley G.Q, & Rowe R.G. (2022). Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axis. Cell reports, 39(1), 110587.
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4

Comprehensive Immune Cell Profiling

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The following antibodies were used (all from BioLegend): CD3 (clone 17A2), Ter119 (TER-119), Gr-1 (RB6–8C5), B220 (RA3–6B2) conjugated to Pacific Blue, CD117 APC-Cy7 (2B8), Sca-1 PE-Cy7 (D7), Flk2 PE (A2F10), CD48 FITC (HM48–1), CD150 APC (TC15–12F12.2), B220 PE-Cy7 (RA3–6B2), Mac-1 PE-Cy5 (M1/70), CD3 PE (17A2), Ter119 APC-Cy7 (TER-119), CD45.2 FITC (104), CD45.1 APC-Cy7 (A20), CD16/32 PerCP-Cy5.5 (93), CD5 APC-Cy7 (53–7.3). CD19 PE (eBio1D3) and CD34 FITC (RAM34) were from eBioscience. Cells were labeled with SYTOX Blue viability stain (Thermo). Data were acquired on LSRII or LSR Fortessa instruments (BD Biosciences). APC-annexin V was from BioLegend.
Wang D., Tanaka-Yano M., Meader E., Kinney M.A., Morris V., Lummertz da Rocha E., Liu N., Liu T., Zhu Q., Orkin S.H., North T.E., Daley G.Q, & Rowe R.G. (2022). Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axis. Cell reports, 39(1), 110587.
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5

Embryonic Hematopoietic Lineage Analysis

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FLs were dissected from E14.5 embryos and disaggregated into a single cell suspension by pipetting. Cell lineages were stained with purified antibodies purchased from BD Biosciences against the following antigens: CD4, CD8, Gr‐1, B220, and Ter119. Conjugated antibodies (Streptavidin‐APC‐Cy7, c‐Kit‐APC, Sca‐1‐PE‐Cy7, CD150‐PerCP‐Cy5.5, and CD48‐FITC) were purchased from Biolegend. DAPI was used to distinguish live cells from dead cells. Cell sorting was performed using a BD Influx cell sorter.
Geng X., Zhang C., Li M., Wang J., Ji F., Feng H., Xing M., Li F., Zhang L., Li W., Chen Z., Hickson I.D., Shen H, & Ying S. (2022). PICH Supports Embryonic Hematopoiesis by Suppressing a cGAS‐STING‐Mediated Interferon Response. Advanced Science, 9(7), 2103837.
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