The primary antibodies used were mouse Discs Large (1:50, 4F3), mouse Armadillo (1:50, N27A1), mouse Prospero (1:100, MR1A), mouse Wingless (1:500, 4D4), and mouse Engrailed (1:50, 4D9) from the Developmental Studies Hybridoma Bank, chicken GFP (1:10000 catalog # ab13970) from Abcam, rabbit GFP (1:500, catalog # A-11122) and mouse V5 (1:500, SV5-Pk1, catalog # R960-25) from Thermo Fisher Scientific, rabbit dsRed (1:500 for Scar-Sens, catalog # 632496) from Clontech/TaKaRa, mouse beta-galactosidase (1:500, catalog # Z378B) from Promega, rabbit phospho-histone H3 (1:1000, Ser10, catalog # 06-570) from Millipore, rabbit Arrow (1:5000)86 (link) and guinea pig Senseless (1:2000)33 (link). The secondary antibodies used were goat or donkey Alexa Fluor 488, 555, or 647 conjugates (1:500) from Invitrogen, and goat or donkey Cy5 conjugates (1:500) from Life Technologies/Jackson Immunochemicals.
Rabbit gfp
Manufactured by Abcam
Rabbit GFP is a recombinant green fluorescent protein (GFP) derived from the jellyfish Aequorea victoria. It is used as a fluorescent marker in various biological applications.
Automatically generated - may contain errors
2 protocols using rabbit gfp
1
Antibody Panel for Developmental Studies
2
Visualizing Biocytin-Labeled and ChR2-Expressing Neurons
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After electrophysiological experiments, individual slices were fixed in 4% paraformaldehyde in PBS over night at 4 °C. The slices used for visualization of biocytin-labeled cells were first rinsed in potassium-PBS and then incubated in 10% goat serum for 1 h followed by a 4 °C overnight incubation of rabbit-GFP (Abcam) primary antibody in 5% goat serum. After rinsing the slices the following day, they were incubated for 2 h with the Rabbit-Cy2 and Streptavidine-Cy5 secondary antibodies with 5% goat serum at room temperature. The slices were mounted on glass slides with 1,4-diazabicyclo[2.2.2]octane (DABCO) as mounting medium. Slices used for visualization of ChR2-expression were sub-sliced to a thickness of 30 μm on a microtome and then processed in a similar way but with chicken-GFP (Chemicon) and rabbit-PV (Swant) as primary antibodies with overnight incubation at room temperature. Chicken-Alexa 488 and rabbit-Cy3 as secondary antibodies were applied for 2 h.
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