Shrna plasmid transfection reagent sc 108061

Manufactured by Santa Cruz Biotechnology

The ShRNA Plasmid Transfection Reagent (sc-108061) is a laboratory product designed for the transfection of short hairpin RNA (shRNA) plasmid DNA into mammalian cells. It is a ready-to-use solution formulated to facilitate the efficient delivery of shRNA plasmids into the target cells.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Ags cell line, by American Type Culture Collection (1 mentions) Pcdna3.1 vector, by GenePharma (1 mentions) Control shrna sc 108060, by Santa Cruz Biotechnology (1 mentions) Ges 1 cell line, by Shanghai Cell Bank (1 mentions) Hsp27 shrna plasmid sc 2935 sh, by Santa Cruz Biotechnology (1 mentions) Sc 29350 sh, by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Transfection reagent (87 citations) ShRNA Plasmid Transfection Reagent (10 citations) Sc-108061 (6 citations) Plasmid transfection reagent (5 citations) ShRNA transfection reagent (4 citations) ShRNA plasmids (4 citations)

4 protocols using shrna plasmid transfection reagent sc 108061

Modulation of SPRR2B Expression in Gastric Cancer Cells

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Human gastric epithelium GES-1 cell line was purchased from the Shanghai Cell Bank of the Chinese Academy of Science (Shanghai, China) and kept in DMEM medium. AGS cell line was obtained from ATCC and kept in F-12K medium. MKN28 and MKN45 cells were obtained from ATCC and maintained in RPMI 1640 medium. All medium was supplemented with 10% fetal bovine serum and penicillin-streptomycin antibiotics.
Human SPRR2B-shRNA (sc-88,442-SH) and control-shRNA (sc-108,060) plasmids were obtained from Santa Cruz Biotechnology. MKN45 cells were transfected with shRNA plasmids using shRNA Plasmid Transfection Reagent (sc-108,061, Santa Cruz).19 (link) Transfected cells were selected by using puromycin. Specific siRNAs targeting MDM2 (sc-29,394) and SPRR2B (sc-88,442) were used for transient transfection, using non-specific scramble siRNA (sc-37,007) as negative control. For overexpression assays, pcDNA3.1-vector, pcDNA3.1-SPRR2B, and pcDNA3.1-SPRR2B-optimized plasmids were synthesized by Gene Pharma (Shanghai, China). The pcDNA3.1-SPRR2B-optimized (pcDNA-SPRR2B°^pt) construct was generated by mutating the SPRR2B-siRNA targeted region of wild type SPRR2B and was used for rescue experiments.

Yao L., Yan J., Cheng F., Gan L., Huang Y., Zheng L, & Fang N. (2021). Small Proline-Rich Protein 2B Facilitates Gastric Adenocarcinoma Proliferation via MDM2-p53/p21 Signaling Pathway. OncoTargets and therapy, 14, 1453-1463.

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Stable Cell Lines for HSP27 Knockdown

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Lentiviruses were used to create stable L-132 cell lines expressing shRNA for HSP27 with a puromycin-resistance gene. HSP27 shRNA plasmid (sc-2935-SH) and shRNA Plasmid Transfection Reagent (sc-108061) were from Santa Cruz Biotechnology. To generate sh-control and sh-HSP27 cells, cell lines were transduced with 1 mol of lentivirus, and selected by puromycin (1 g/mL) for at least one week.

Kim J.Y., An Y.M., Yoo B.R., Kim J.M., Han S.Y., Na Y., Lee Y.S, & Cho J. (2018). HSP27 inhibitor attenuates radiation-induced pulmonary inflammation. Scientific Reports, 8, 4189.

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Establishing Stable HSP27 Knockdown in NSCLC

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Human non-small cell lung cancer cell line NCI-H460 was cultured in RPMI supplemented with 10% fetal bovine serum and 1% penicillin streptomycin in a 37°C incubator with 5% CO₂. Transfections were performed using Lipofectamine 2000 (Invitrogen). Lentiviruses were used to create stable NCI-H460 cell lines expressing shRNA for HSP27 with a puromycin-resistance gene. The HSP27 shRNA plasmid (sc-2935-SH) and shRNA Plasmid Transfection Reagent (sc-108061) were from Santa Cruz Biotechnology. To generate the sh-control and sh-HSP27 cells, cell lines were transduced with 1 mol lentivirus and selected using puromycin (1 μg/mL) for at least one week.

Choi B., Choi S.K., Park Y.N., Kwak S.Y., Lee H.J., Kwon Y., Na Y, & Lee Y.S. (2017). Sensitization of lung cancer cells by altered dimerization of HSP27. Oncotarget, 8(62), 105372-105382.

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Establishment of NCI-H460 Cell Lines with Reduced HSP27

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The human non–small lung cancer cell line NCI-H460 was cultured in RPMI (Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (Gibco) in a 37°C incubator with 5% CO₂. Transfections were performed using Lipofectamine 2000 (Invitrogen, Carlsbad, CA USA). Lentiviruses were used to create stable NCI-H460 cell lines expressing shRNA for HSP27 (puromycin resistance gene). The HSP27 shRNA plasmid (sc-29350-SH), and shRNA plasmid transfection reagent (sc-108061) were ordered from Santa Cruz Biotechnology. To generate the sh-Control, and sh-HSP27 cells, the cell lines were transduced with 1 mol of lentivirus and selected using puromycin (1 ug/mL) for at least one week.

Kim J.H., Jung Y.J., Choi B., Lee N.L., Lee H.J., Kwak S.Y., Kwon Y., Na Y, & Lee Y.S. (2016). Overcoming HSP27-mediated resistance by altered dimerization of HSP27 using small molecules. Oncotarget, 7(33), 53178-53190.

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