Log In Sign up
The largest database of trusted experimental protocols

Micro tube 1.1 ml z gel

Manufactured by Sarstedt
Visit Supplier

The Micro tube 1.1 ml Z-Gel is a laboratory equipment product manufactured by Sarstedt. It is a 1.1 ml capacity tube designed for various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

C57bl 6 mice, by Jackson ImmunoResearch (2 mentions) Luminex 200 system, by Bio-Rad (1 mentions) Bio plex pro mouse cytokine 23 plex assay, by Bio-Rad (1 mentions) C57bl 6 mice, by Janvier Labs (1 mentions)

Close spelling variants of this product

Z-Gel (8 citations) Z-Gel tubes (6 citations) Micro Z-gel tubes (4 citations)

4 protocols using micro tube 1.1 ml z gel

1

Serum Cytokine Response to TRT

Check if the same lab product or an alternative is used in the 5 most similar protocols
To analyze the immediate inflammatory response to TRT on serum cytokine levels, 50 to 100 μL blood was collected via retro-orbital bleeding 6 hours after the last treatment. Blood was transferred into a serum separation tube (Micro tube 1.1 mL Z-Gel, Sarstedt). The tubes were centrifuged at approximately 10,000 rcf for 5 minutes and serum was transferred into a clean Eppendorf tube. Cytokine content was determined using the Bio-Plex Pro Mouse Cytokine 23-plex Assay (Bio-Rad, RRID:AB_2857368) using a Luminex 200 System (Bio-Rad, RRID:SCR_018025).
Ertveldt T., De Beck L., De Ridder K., Locy H., de Mey W., Goyvaerts C., Lecocq Q., Ceuppens H., De Vlaeminck Y., Awad R.M., Keyaerts M., Devoogdt N., D'Huyvetter M., Breckpot K, & Krasniqi A. (2022). Targeted Radionuclide Therapy with Low and High-Dose Lutetium-177–Labeled Single Domain Antibodies Induces Distinct Immune Signatures in a Mouse Melanoma Model. Molecular Cancer Therapeutics, 21(7), 1136-1148.
+ Open protocol
+ Expand
2

Temporal Immune Profiling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
C57BL/6 mice, all female, 6-8 weeks old, were purchased from Janvier Labs, France. B6. SJL-Ptprca Pepcb/BoyJ (CD45.1) mice were maintained in-house. Mice were sacrificed at 0, 3, 7, 10, and 14 days post-infection or immunization. Blood was collected and dispensed into serum separation tubes (Microtube 1.1 mL Z-Gel, Sarstedt). Collected tissues were transferred into separate tubes containing PBS + 1% BSA for processing.
Besavilla D.F., Reusch L., Enriquez J., Schön K, & Angeletti D. (2023). Pre-existing CD4 T cell help boosts antibody responses but has limited impact on germinal center, antigen-specific B cell frequencies after influenza infection. Frontiers in Immunology, 14, 1243164.
+ Open protocol
+ Expand
3

Serum CCL20LD Quantification in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Eight- to 10-week-old female C57BL/6 mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and utilized in line with institutionally approved animal protocols (University of California Davis, Sacramento, California). To detect serum levels of CCL20LD, each mouse was treated with 400 μg of CCL20LD dissolved in 200 μl of PBS via intraperitoneal, intravenous, or subcutaneous injection. Mice were euthanized after two hours of incubation, followed immediately by a blood draw from the heart. Whole blood was transferred to serum collection tubes (Micro tube 1.1 ml Z-Gel, SARSTEDT, Inc. Newton NC) and left undisturbed for 10 min, followed by centrifugation at 2500 rpm. The resulting supernatant was designated serum for either direct use in ELISA assay or stored as aliquots at −80 °C degree for later use.
Wu X., Clarke W.R., Koplinski C.A., Peterson F.C., Dwinell M.B., Wei G., Chao E., Huynh M., Yamada D., Volkman B.F, & Hwang S.T. (2023). A modified ELISA assay differentiates CCL20 locked dimers from wild-type monomers. Journal of immunological methods, 515, 113453.
+ Open protocol
+ Expand
4

Serum CCL20LD Detection in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Eight-to 10-week-old female C57BL/6 mice were purchased from The Jackson Laboratory (Bar Harbor, ME) and utilized in line with institutionally approved animal protocols (University of California Davis, Sacramento, California). To detect serum levels of CCL20LD, each mouse was treated with 400 μg of CCL20LD dissolved in 200 μl of PBS via intraperitoneal, intravenous, or subcutaneous injection. Mice were euthanized after two hours of incubation, followed immediately by a blood draw from the heart. Whole blood was transferred to serum collection tubes (Micro tube 1.1 ml Z-Gel, SARSTEDT, Inc. Newton NC) and left undisturbed for 10 min, followed by centrifugation at 2500 rpm. The resulting supernatant was designated serum for either direct use in ELISA assay or stored as aliquots at -80 °C degree for later use.
Wu X., Clarke W.R., Koplinski C.A., Peterson F.C., Dwinell M.B., Wei G., Chao E., Huynh M., Yamada D., Volkman B.F, & Hwang S.T. (2023). A modified ELISA assay differentiates CCL20 locked dimers from wild-type monomers. Journal of immunological methods, 515.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!