Ssdna reporter fam ttatt bhq1

All reactions involved primers, ssDNA-reporter (FAM-TTATT-BHQ1), and CRISPR RNA (crRNA) synthesized by Sangon Biotech (Shanghai, China). EngenLba Cas12a, nuclease-free water, and 10 × NEbuffer were purchased from New England Biolabs (Ipswich, MA, United States). The TwistAmp Basic kit (#TABAS03KIT) for RPA was purchased from TwistD× Limited (Maidenhead, United Kingdom). The PCR Master Mix was purchased from Takara (Dalian, China). The Quantitative Real-time PCR (qPCR) Master Mix was purchased from YEASEN (Shanghai, China). The pUC57 recombinant plasmids containing vacA, cagA, and 16SrDNA genes of H. pylori were transformed by E. coli, and the above process was done by Sangon Biotech (Shanghai, China). Genomic DNA Extraction Kits were purchased from TIANGEN Biotech (Beijing, China).
Clinical saliva samples were provided by the Tang du Hospital of Air Force Military Medical University (Xi’an, China). The study was approved by the Air Force Military Medical University Research Ethics Review Board. A written informed (verbal) consent was obtained from all patients. Saliva samples were collected from all volunteers after undergoing ¹³C-UBT.

The RNA standards for SARS-CoV-2, SARS-CoV, Parainfluenza virus, Influenza virus A (FluA), and Influenza virus A (FluB) were synthesized by the Shanghai Institute of Measurement and Testing Technology. All primers, CRISPR RNA (crRNA), and the ssDNA reporter (FAM-TTATT-BHQ1) were synthesized by the Sangon Biotech Co. Ltd., Shanghai, China. EnGen^® Lba Cas12a was purchased from New England Biolabs (Ipswich, MA, UK). The RNase inhibitor was purchased from Takara and the RPA amplification kit was purchased from Leshang Biotechnology (Wuxi) Co., LTD., China.