3 o acetyl n4 benzoyl 2 deoxycytidine

Capecitabine, cytidine, cytosine, N⁴-acetylcytosine, and cytosine β-d-arabinofuranoside were obtained from Sigma-Aldrich (Germany). N⁴-acetyl-2′-deoxy-5′-O-DMT-cytidine, N⁴-benzoylcytidine, N⁴-benzoyl-5-methylcytidine, 2′-deoxy-5-hydroxycytidine, 2′-deoxy-5-hydroxymethylcytidine, 5-hydroxymethylcytidine, 2′-deoxy-5-propynylcytidine, pseudoisocytidine, isocytidine, 5-fluorocytidine, 2-thiocytidine, 2′-deoxy-5-methylcytidine, 2′,5′-dideoxycytidine, 2′,3′-dideoxycytidine, 2′-O-methylcytidine, 3′-azido-N⁴-benzoyl-2′,3′-dideoxycytidine, and N³-methyl-2′-deoxy cytidine were purchased from Carbosynth (UK); 3′-O-levulinoyl-N⁴-benzoyl-2′-deoxycytidine, 5′-O-levulinoyl-N⁴-benzoyl-2′-deoxycytidine, 3′-O-acetyl-N⁴-benzoyl-2′-deoxycytidine, S⁴-n-propylthiouridine-5′-triphosphate, S⁴-iso-propylthiouridine-5′-triphosphate, and S⁴-iso-butylthiouridine-5′-triphosphate were obtained from Jena Bioscience. S⁴-n-propylthiouridine-5′-triphosphate, S⁴-iso-propylthiouridine-5′-triphosphate, and S⁴-iso-butylthiouridine-5′-triphosphate were dephosphorylated using FastAP alkaline phosphatase (Thermo Fisher Scientific) before treatment with CDAs. N⁴-acetylcytidine, N⁴-acetyl-2′-deoxycytidine, N⁴-benzoyl-2′-deoxycytidine, and N⁴-isobutyryl-2′-deoxycytidine were purchased from Combi-Blocks (USA), and N⁴-benzoylcytosine was purchased from Bide Pharmatech Ltd.

Restriction endonucleases, HindIII, BamHI, PstI, Phusion DNA polymerase, aLICator™ LIC Cloning and Expression System Kit 3, PageRuler^TM Prestained Protein Ladder were purchased from Thermo Fisher Scientific, Vilnius, Lithuania, Pierce™ Coomassie Plus (Bradford, UK) Assay Reagent and HisPur™ Ni‐NTA spin column were purchased from Thermo Fisher Scientific, Rockford, IL. pET21a Vector was purchased from Novagen (Madison, USA). Nutrient medium were purchased Roth, Germany. pNP‐acyl esters, tributyrin, β‐d‐glucose pentaacetate, β‐d‐galactose pentaacetate, and uridine were purchased from Sigma‐Aldrich. ‘ZR Soil Microbe DNA MidiPrep™’ was purchased from Zymo Research, Freiburg, Germany. Nitrocefin was purchased from Oxoid, UK. 3′‐O‐benzoyl‐2′‐deoxyuridine were purchased from Carbosynth, UK. 3′‐O‐acetyl‐2′‐deoxyuridine, 3′‐O‐acetyl‐N⁴‐benzoyl‐2′‐deoxycytidine, 3′‐O‐levulinyl‐N⁴‐benzoyl‐2′‐deocytidine, and 5′‐O‐levulinyl‐N⁴‐benzoyl‐2′‐deocytidine were purchased from Jena Bioscience, Jena, Germany.