b6 cg, by Jackson ImmunoResearch - Product details

1

Investigating SIRT2 Knockout Mice

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C57BL/6J mice, Pmel mice with gp100-reactive TCR:
B6.Cg-Thy1^a/Cy Tg(TcraTcrb)8Rest/J, OT-II
mice with MHC II-restricted OVA-specific TCR: B6.Cg-Tg(TcraTcrb)425Cbn/J,
Sirt2^−/− mice:
B6.129-Sirt2^tm1.1Fwa/J and NSG mice:
NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/SzJ were purchased from The Jackson
Laboratory. Pmel mice and OT-II mice were crossed with
Sirt2^−/− mice to generate
Sirt2^−/− Pmel mice and
Sirt2^−/− OT-II mice,
respectively. All mice were bred and maintained under specific pathogen-free
conditions at the animal facility of H. Lee Moffitt Cancer Center. All
animal protocols were approved by the Institutional Animal Care and Use
Committee (IACUC). Mice were used at 7–8 weeks of age with age- and
sex-matched controls.

Hamaidi I., Zhang L., Kim N., Wang M.H., Iclozan C., Fang B., Liu M., Koomen J.M., Berglund A.E., Yoder S.J., Yao J., Engelman R.W., Creelan B.C., Conejo-Garcia J.R., Antonia S.J., Mulé J.J, & Kim S. (2020). Sirt2 Inhibition Enhances Metabolic Fitness and Effector Functions of Tumor-Reactive T cells. Cell metabolism, 32(3), 420-436.e12.

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2

Generation of Transgenic Mouse Strains

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The following mouse strains used were purchased from Jackson Laboratory and/or bred at our facility: C57BL/6J (B6); B6.129P2-Tcrb^tm1Mom/J (Tcrb^−/−); B6.129S2-Il1b^tm1Dch (Il1b^−/−); B6.Cg-Tg-Il17f^{tm1(Thy1)Weav} (Il17f^thy1.1); B6.Cg-Tg^{(TcraTcrb)425Cbn}/J (OT-II); B6N.129(Cg)-Foxp3^tm3Ayr/J (Foxp3^gfp); B6.129S7-Il1r1^tm1Imx/J (Il1r1^−/−); B6.129S6-Stat5a-Stat5b^tm2Mam/Mmjax (Stat5^fl/fl); B6.129S1-Rela^fl/fl (Rela^fl/fl); B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J (Rosa26^{TdTomato fl/+}); and B6.129S4-Socs3^tm1Ayos/J (Socs3^fl/fl). B6.Cg-Tg(Cd4-Cre)^1Cwi (Cd4-Cre) were purchased from Taconic and B6.CD45.1 from Frederick Cancer Center. All strains were on a B6 background. All intercrosses to generate additional strains like WT and IL-1R-deficient dual reporter mice (Il17f^thy1.1.Foxp3^gfp), Il17f^thy1.1.CD45.1/CD45.2 and Stat5b^fl/fl. Cd4-Cre mice were generated by crosses in our breeding facility. Animals were bred and maintained under specific pathogen-free condition in accordance with institutional animal care and use committee regulations.

Basu R., Whitley S.K., Bhaumik S., Zindl C.L., Schoeb T.R., Benveniste E.N., Pear W.S., Hatton R.D, & Weaver C.T. (2015). IL-1 signaling modulates STAT activation to antagonize retinoic acid signaling and control Th17–iTreg balance. Nature immunology, 16(3), 286-295.

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3

Genetically Modified Mouse Strains for Cell Lineage Tracking

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C57BL/6J (wild type, WT), B6.SJL-Ptprc^aPepc^b/BoyJ (CD45.1⁺), C57BL/6-Tg(UBC-GFP)30Scha/J (GFP⁺), B6.129X1-Nfe2l2^tm1Ywk/J (Nrf2^−/−), B6.Cg-Msr1^tm1Csk/J (Msr1^−/−), B6.129S1-Cd36^tm1Mfe/J (CD36^−/−), B6.129-Hba^tm1(HBA)Tow/Hbb^{tm2(HBG1,HBB*)Tow} (sickle cell disease, SCD) and B6.129-Hba^tm1(HBA)Tow/Hbb^{tm3(HBG1,HBB*)Tow} (non-sickling transgenic control, tgCtrl.) mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). GM-CSF-deficient mice (Csf2^−/−) on a C57BL/6J background were bred in-house. B6.129S6-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J (ROSA26-tdTomato^fl/fl or R26-tdT) and B6.129P2(Cg)-B6.129P2(C)-Cx3cr1^{tm2.1(cre/ERT2)Jung}/J (Cx3cr1-CreERT2) ^{24 (link),25 (link)} were kindly provided by Prof. Dr. M. Prinz. Mice carrying the myeloid-specific knockout of the FPN1 gene (LysM^Cre/+ Slc40a1^fl/fl) were kindly provided by Dr. F. Wang, back-crossed on a C57BL/6 background and bred in house. Unless otherwise indicated age- and sex-matched animals were used at 8 – 12 weeks of age. Where appropriate, animals were randomly assigned to interventions. All protocols were approved by the Animal Review Committee at Massachusetts General Hospital (Protocol No. 2011N000035 and 2015N000044) and by the Federal Ministry of Science, Research and Economy in Austria (Project No. BMWFW-66.011/0115-WF/V/3b/2014).

Theurl I., Hilgendorf I., Nairz M., Tymoszuk P., Haschka D., Asshoff M., He S., Gerhardt L.M., Holderried T.A., Seifert M., Sopper S., Fenn A.M., Anzai A., Rattik S., McAlpine C., Theurl M., Wieghofer P., Iwamoto Y., Weber G.F., Harder N.K., Chousterman B.G., Arvedson T.L., McKee M., Wang F., Lutz O.M., Rezoagli E., Babitt J.L., Berra L., Prinz M., Nahrendorf M., Weiss G., Weissleder R., Lin H.Y, & Swirski F.K. (2016). On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver. Nature medicine, 22(8), 945-951.

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4

Transgenic Mouse Lines for Lineage Tracing

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B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J (007914), B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J (007676), B6D2F1/J (100006), B6.Cg-Pdgfrbtm1.1(cre/ERT2)Csln/J (030201), B6.Cg-Tg(Tek-cre)1Ywa/J (008863), B6.129P2(C)-Cx3cr1tm2.1(cre/ERT2)Jung/J (020940), and B6.129S4-Gt(ROSA)26Sortm2(FLP*)Sor/J (012930) were obtained from Jackson Laboratories. Hoxa13^Cre-WPRE and Hoxa13^Cre mice generated in this study were described below. For timed matings, embryonic day 0.5 (E0.5) was identified by the time a vaginal plug was discovered. The pregnant dams at the indicated times were sacrificed and the embryo and placenta were dissected out from the uterus. All experiments were performed at least twice using different litters and used littermate controls on a mixed background unless otherwise indicated. These mice were maintained in a specific pathogen-free environment under a 14/10 hour light/dark cycle and all animal experiments described were performed in accordance and approval of the University of Pennsylvania Institutional Animal Care and Use Committee.

Chen X., Tang A.T., Tober J., Yang J., Leu N.A., Sterling S., Chen M., Yang Y., Mericko-Ishizuka P., Speck N.A, & Kahn M.L. (2022). Mouse placenta fetal macrophages arise from endothelial cells outside the placenta. Developmental cell, 57(23), 2652-2660.e3.

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5

Mouse Strain Characterization for Immunology Research

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C57BL/6 and B6D2F1/J mice were purchased from Charles River Laboratories at the age of seven weeks and housed in our facility for at least one week before being used in experiments. B6.129S(C)-Batf3^tm1Kmm/J, B6(Cg)-Irf8^tm1.1Hm/J, B6.129S4-Pten^tm1Hwu/J, B6.129P2(C)-Ccr7^tm1Rfer/J, B6.129S7-Ifng^tm1Ts/J, B6.129S7-Ifngr1^tm1Agt/J, B6(Cg)-Ifnar1^tm1.2Ees/J, B6.129S7-Il1r1^tm1lmx/J, B6.129P2(SJL)-Myd88^tm1.1Befr/J, B6.129S7-Rag1^tm1Mom/J and C57BL/6J-Ticam1^Lps2/J mice were purchased from Jackson Laboratories and bred in our facility or used for experiments after at least one week of housing in our facility. Cd207-Cre mice were provided by B. Clausen^{21 (link)}. JEDI mice were provided by B. Brown. Axl^−/− and Axl^−/−Mertk^−/− bone marrow were provided by C. Rothlin and S. Ghosh. Asc^−/− mice were provided by Millenium.
Floxed mice were crossed to Cd207-Cre mice in our facility. Mice were maintained at specified pathogen-free (SPF) health status in individually ventilated cages at 21–22 °C and 39–50% humidity. Male mice at the age of 8–12 weeks were used for experiments. All animal procedures were approved by the Institutional Animal Care and Use Committees (IACUCs) of the respective institutions.

Maier B., Leader A.M., Chen S.T., Tung N., Chang C., LeBerichel J., Chudnovskiy A., Maskey S., Walker L., Finnigan J.P., Kirkling M.E., Reizis B., Ghosh S., D’Amore N.R., Bhardwaj N., Rothlin C.V., Wolf A., Flores R., Marron T., Rahman A.H., Kenigsberg E., Brown B.D, & Merad M. (2020). A conserved dendritic-cell regulatory program limits antitumour immunity. Nature, 580(7802), 257-262.

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6

Transgenic Mouse Strains for Muscle Research

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The following mouse strains were obtained from Jackson Laboratories and used in this study: B6.Cg-Pax7^{tm1(cre/ERT2)Gaka}/J (Jax strain number: 017763), B6.Cg-Gt(ROSA)26Sor^{tm75.1(CAG-tdTomato*)Hze}/J (Jax strain number: 025106), B10ScSn.Cg-Prkdc^scidDmd^mdx/J (Jax strain number: 018018). The strains B6.Cg-Pax7^{tm1(cre/ERT2)Gaka}/J and B6.Cg-Gt(ROSA)26Sor^{tm75.1(CAG-tdTomato*)Hze}/J were crossed to homozygosity to obtain Pax7-CreERT2; R26-LSL-ntdTomato reporter MEF lines. The Pax7-nGFP reporter MEF lines were obtained from crossing homozygous Tg:Pax7-nGFP/C57BL6;DBA2 mice^{51 (link)}. All mice used in this study were housed in specific-pathogen-free (SPF)-like conditions according to the Swiss Federal Law on Animal Protection and approved by the Cantonal Animal Welfare Committee (license numbers ZH108/2018, ZH177/2018 and ZH002/2022).

Qabrati X., Kim I., Ghosh A., Bundschuh N., Noé F., Palmer A.S, & Bar-Nur O. (2023). Transgene-free direct conversion of murine fibroblasts into functional muscle stem cells. NPJ Regenerative Medicine, 8, 43.

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7

Generation of Transgenic Mouse Strains

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The following mouse strains used were purchased from Jackson Laboratory and/or bred at our facility: C57BL/6J (B6); B6.129P2-Tcrb^tm1Mom/J (Tcrb^−/−); B6.129S2-Il1b^tm1Dch (Il1b^−/−); B6.Cg-Tg-Il17f^{tm1(Thy1)Weav} (Il17f^thy1.1); B6.Cg-Tg^{(TcraTcrb)425Cbn}/J (OT-II); B6N.129(Cg)-Foxp3^tm3Ayr/J (Foxp3^gfp); B6.129S7-Il1r1^tm1Imx/J (Il1r1^−/−); B6.129S6-Stat5a-Stat5b^tm2Mam/Mmjax (Stat5^fl/fl); B6.129S1-Rela^fl/fl (Rela^fl/fl); B6.Cg-Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/J (Rosa26^{TdTomato fl/+}); and B6.129S4-Socs3^tm1Ayos/J (Socs3^fl/fl). B6.Cg-Tg(Cd4-Cre)^1Cwi (Cd4-Cre) were purchased from Taconic and B6.CD45.1 from Frederick Cancer Center. All strains were on a B6 background. All intercrosses to generate additional strains like WT and IL-1R-deficient dual reporter mice (Il17f^thy1.1.Foxp3^gfp), Il17f^thy1.1.CD45.1/CD45.2 and Stat5b^fl/fl. Cd4-Cre mice were generated by crosses in our breeding facility. Animals were bred and maintained under specific pathogen-free condition in accordance with institutional animal care and use committee regulations.

Basu R., Whitley S.K., Bhaumik S., Zindl C.L., Schoeb T.R., Benveniste E.N., Pear W.S., Hatton R.D, & Weaver C.T. (2015). IL-1 signaling modulates STAT activation to antagonize retinoic acid signaling and control Th17–iTreg balance. Nature immunology, 16(3), 286-295.

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Comprehensive Mouse Strain Repository for Immunology

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All mice were on the C57BL/6J (B6) background and maintained in accordance with the animal care and use regulations of the University of Chicago. The following mouse strains were purchased from the Jackson Laboratory and maintained in our facility: C57BL/6J (B6), CD45.1⁺ B6.SJL-Ptprc^a Pepc^b/BoyJ, Aire^−/− B6.129S2-Aire^tm1.1Doi/J, Foxp3^GFP B6.Cg-Foxp3^tm2Tch/J, Rag1^−/− B6.129S7-Rag1^tm1Mom/J, Itgax-DTR B6.FVB-Tg(Itgax-DTR/EGFP) 57Lan/J, BDCA2-DTR C57BL/6-Tg(Clec4b1-HBEGF)956Cln/J, MHC-II deficient B6.129S2-H2^dlAb1-Ea/J, Cd80^−/−Cd86^−/− B6.129S4-Cd80^tm1ShrCd86^tm2Shr/J, Ccr7^−/− B6.129P2(C)-Ccr7^tm1Rfor/J, Itgax-Cre B6.Cg-Tg(Itgax-cre)1-1Reiz/J, Ab1^flox/flox B6.129X1-H2-Ab1^tm1Koni/J, Ab1^−/− B6.129-H2-Ab1^tm1Gru/Tac, Batf3^−/− B6.129S(C)-Batf3^tm1Kmm/J, and Ighm^−/− B6.129S2-Ighm^tm1Cgn/J. MJ23Tg, RT83Tg, and “TCRβTg” mice were generated as described previously (Malchow et al., 2013 (link)).

Leventhal D.S., Gilmore D.C., Berger J.M., Nishi S., Lee V., Malchow S., Kline D.E., Kline J., Griend D.J., Huang H., Socci N.D, & Savage P.A. (2016). Dendritic cells coordinate the development and homeostasis of organ-specific regulatory T cells. Immunity, 44(4), 847-859.

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9

CCHFV Infection in Gene-Deficient Mice

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C57BL/6J (BL6), B6.129, MAVS KO (B6;129-Mavstm1Zjc/J), MDA5 KO (B6.Cg-Ifih1tm1.1Cln/J), TNF-α KO (B6;129S-Tnftm1Gkl/J), IL-6 KO (B6.129S2-Il6tm1Kopf/J), TNFAR DBL KO (B6.129S-Tnfrsf1atm1Imx Tnfrsf1btm1Imx/J), p55 KO (C57BL/6-(Tnfrsf1atm1Imx/J), p75 KO (B6.129S7-Tnfrsf1btm1Imx/J) and Rag2 KO (B6.Cg-Rag2tm1.1Cgn/J) mice (6–8 weeks old) were purchased from The Jackson Laboratory. Mice were challenged with 100 PFU of the indicated CCHFV strain by IP injection of virus diluted in a total volume of 0.2 ml Phosphate buffered saline (PBS). To induce the IFN-I antibody blockade, mice were injected by the IP route with 2.5 mg of anti-IFNR1 (MAb-5A3) (Leinco Technologies, Inc) diluted in PBS 24 h post-virus exposure as previous described [19 (link)].

Golden J.W., Zeng X., Cline C.R., Smith J.M., Daye S.P., Carey B.D., Blancett C.D., Shoemaker C.J., Liu J., Fitzpatrick C.J., Stefan C.P, & Garrison A.R. (2022). The host inflammatory response contributes to disease severity in Crimean-Congo hemorrhagic fever virus infected mice. PLoS Pathogens, 18(5), e1010485.

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Genetically Modified Mouse Strains for Cell Lineage Tracking

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C57BL/6J (wild type, WT), B6.SJL-Ptprc^aPepc^b/BoyJ (CD45.1⁺), C57BL/6-Tg(UBC-GFP)30Scha/J (GFP⁺), B6.129X1-Nfe2l2^tm1Ywk/J (Nrf2^−/−), B6.Cg-Msr1^tm1Csk/J (Msr1^−/−), B6.129S1-Cd36^tm1Mfe/J (CD36^−/−), B6.129-Hba^tm1(HBA)Tow/Hbb^{tm2(HBG1,HBB*)Tow} (sickle cell disease, SCD) and B6.129-Hba^tm1(HBA)Tow/Hbb^{tm3(HBG1,HBB*)Tow} (non-sickling transgenic control, tgCtrl.) mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). GM-CSF-deficient mice (Csf2^−/−) on a C57BL/6J background were bred in-house. B6.129S6-Gt(ROSA)26Sor^{tm9(CAG-tdTomato)Hze}/J (ROSA26-tdTomato^fl/fl or R26-tdT) and B6.129P2(Cg)-B6.129P2(C)-Cx3cr1^{tm2.1(cre/ERT2)Jung}/J (Cx3cr1-CreERT2) ^{24 (link),25 (link)} were kindly provided by Prof. Dr. M. Prinz. Mice carrying the myeloid-specific knockout of the FPN1 gene (LysM^Cre/+ Slc40a1^fl/fl) were kindly provided by Dr. F. Wang, back-crossed on a C57BL/6 background and bred in house. Unless otherwise indicated age- and sex-matched animals were used at 8 – 12 weeks of age. Where appropriate, animals were randomly assigned to interventions. All protocols were approved by the Animal Review Committee at Massachusetts General Hospital (Protocol No. 2011N000035 and 2015N000044) and by the Federal Ministry of Science, Research and Economy in Austria (Project No. BMWFW-66.011/0115-WF/V/3b/2014).

Theurl I., Hilgendorf I., Nairz M., Tymoszuk P., Haschka D., Asshoff M., He S., Gerhardt L.M., Holderried T.A., Seifert M., Sopper S., Fenn A.M., Anzai A., Rattik S., McAlpine C., Theurl M., Wieghofer P., Iwamoto Y., Weber G.F., Harder N.K., Chousterman B.G., Arvedson T.L., McKee M., Wang F., Lutz O.M., Rezoagli E., Babitt J.L., Berra L., Prinz M., Nahrendorf M., Weiss G., Weissleder R., Lin H.Y, & Swirski F.K. (2016). On-demand erythrocyte disposal and iron recycling requires transient macrophages in the liver. Nature medicine, 22(8), 945-951.

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B6 cg

Lab products found in correlation

10 protocols using b6 cg

Investigating SIRT2 Knockout Mice

Generation of Transgenic Mouse Strains

Genetically Modified Mouse Strains for Cell Lineage Tracking

Transgenic Mouse Lines for Lineage Tracing

Mouse Strain Characterization for Immunology Research

Transgenic Mouse Strains for Muscle Research

Generation of Transgenic Mouse Strains

Comprehensive Mouse Strain Repository for Immunology

CCHFV Infection in Gene-Deficient Mice

Genetically Modified Mouse Strains for Cell Lineage Tracking

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