In our studies we used TgAPT121 mice (TgAPT121+/−, C57BL/6 × DBA/2 background) (19 (link)) bred to male B6D2F1 mice (Jackson Laboratories, Bar Harbor, ME). This model has probasin promoter driven, prostate epithelial cell-specific expression of a truncated SV40 Large T antigen protein that inactivates pRb family proteins. It has strong histologic similarities to the earlier stages of human prostate cancer (i.e. demonstrating hyperplasia, PIN, and adenocarcinoma over 6 months). Other mouse models used include mice with a floxed exon 2 in the Vdr gene (Vdrfl/fl; C57BL/6) (18 (link)); mice with Cre recombinase expression driven by the rat probasin promoter (PB-Cre+/−; C57BL/6) (NCI Mouse Models of Human Cancer Consortium, # 01XF5; Frederick, MD); and Vdr knockout mice with intestine-specific transgenic expression of the human VDR gene (C57BL/6) (20 (link)). Mice were genotyped as previously described (18 (link)–21 (link)). Mice were housed with a 12 h light/12 h dark cycle, in shoebox cages with individual ventilation. Lights were covered with a UVB filter (Pegasus Associates Lighting, Beaver, PA). Diets and water were fed ad libitum. Except for Experiment 4, mice were fed AIN-76A diet (1000 IU vitamin D3/kg diet (22 (link))). All experiments were approved by either the Purdue University or the Ohio State University Animal Care and Use Committees.
B6d2f1 mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
B6D2F1 mice are a hybrid mouse strain that is commonly used in research. These mice are the result of a cross between C57BL/6 (B6) and DBA/2 (D2) mouse strains. B6D2F1 mice exhibit characteristics of both parental strains and are often used as a model organism in various research applications.
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Lab products found in correlation
C57bl 6, by Jackson ImmunoResearch (4 mentions)
C57bl 6 mice, by Thermo Fisher Scientific (3 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (3 mentions)
Iron dextran, by Merck Group (1 mentions)
Dextrose, by Merck Group (1 mentions)
Nod cg prkdcscid il2rgtm1wjl szj nsg mice, by Jackson ImmunoResearch (1 mentions)
Athymic nude nu j mice, by Jackson ImmunoResearch (1 mentions)
Ksom aa, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
12 protocols using b6d2f1 mice
1
Genetically Modified Mouse Models of Prostate Cancer
2
Establishing Iron-Loaded Mouse Model
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Iron-loaded mouse model was generated as described previously [24 (link)]. Seven-week-old male B6D2F1 mice were purchased from The Jackson Laboratory (Bar Harbor, Maine, USA). This part of the study was approved by the Department of Health, Hong Kong SAR, and the Committee on the Use of Live Animals in Teaching and Research, The University of Hong Kong. The investigations conform with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health and the ethical policy of our institution is listed as being compliant with that of NIH (assurance number A5773-01). Mice were acclimatized for at least one week before experiments. The 8-week-old male B6D2F1 mice were given intra-peritoneal injection of iron dextran (50 mg iron/ml; Sigma, St Louis, MO, USA), at 3.1 mg per 25 g body wt per day on a 5 days/week schedule, for 13 weeks. Control mice received 10% dextrose (Sigma, St Louis, MO, USA). Mice were sacrificed by an overdose of isoflurane anaesthesia until lack of respiration for 5 minutes to ensure euthanasia, followed by cervical dislocation. Ventricular tissues were stored at -80°C until analysis.
3
B6D2F1 Mice Study Protocol
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Eight-week-old male B6D2F1 mice were purchased from the Jackson Laboratory. Animals were kept under standard laboratory conditions and allowed free access to water and food except as noted. All experiments were performed with the approval of the appropriate institutional animal care and use committee.
4
Transgenic Mouse Breeding and Housing
5
Bone Marrow Transplantation in Mice
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Example 76
Female C57BL/6 and B6D2F1 mice are purchased from the Jackson Laboratory (Bar Harbor, Me., USA). Bone marrow is harvested from the femurs and tibias of 12-20 week old mice. Before receiving transplantation, B6D2F1 mice are given 14 Gy total body irradiation (137Cs source). Irradiation is done twice, three hours apart. 5×10{circumflex over ( )}6 bone marrow cells are supplemented with 2×10{circumflex over ( )}6 nylon-wool nonadherent splenetic T cells from C57BL/6 mice and resuspended in Leibovitz's L15 medium (Life Technologies Inc., New York USA) and transplanted by tail vein infusion (0.25 mL) into B6D2F1 mice (Cooke K R, Gerbitz A, Crawford J M, Teshmia T, Hill G R, Tesolin A. Rossignol D P, Ferrara J L M, 2001. LPS antagonism reduces graft-versus-host disease and preserves graft-versus-host leukemia after experimental bone marrow transplantation. The Journal of Clinical Investigation. 107:1581-1589).
6
Efficacy of Oncolytic HSV in NSG Mice
7
Bone Marrow Transplant in Mice
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Example 76
Female C57BL/6 and B6D2F1 mice are purchased from the Jackson Laboratory (Bar Harbor, Me., USA). Bone marrow is harvested from the femurs and tibias of 12-20 week old mice. Before receiving transplantation, B6D2F1 mice are given 14 Gy total body irradiation (137Cs source). Irradiation is done twice, three hours apart. 5×10 6 bone marrow cells are supplemented with 2×10 6 nylon-wool nonadherent splenetic T cells from C57BL/6 mice and resuspended in Leibovitz's L15 medium (Life Technologies Inc., New York USA) and transplanted by tail vein infusion (0.25 mL) into B6D2F1 mice (Cooke K R, Gerbitz A, Crawford J M, Teshmia T, Hill G R, Tesolin A, Rossignol D P, Ferrara J L M 2001. LPS antagonism reduces graft-versus-host disease and preserves graft-versus-host leukemia after experimental bone marrow transplantation. The Journal of Clinical Investigation. 107:1581-1589).
8
Athymic Nude Mice for Preclinical Studies
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Athymic nude NU/J mice from Jackson Laboratories (Bar Harbor, Maine) were used for imaging (n = 8) and pharmacokinetic studies (n = 48). Athymic nude CrTac:NCr-Foxn1nu mice from Taconic Laboratories (Hudson, NY) were used to determine the stability of PARPi-FL (n = 18). They were further used for orthotopic mouse models (n = 24). For in vivo toxicology, B6D2F1 mice from Jackson Laboratories (Bar Harbor, Maine) were used. For subcutaneous injections, mice were anesthetized with 2% isoflurane gas in 2 L/min medical air. For orthotopic injections, mice were anesthetized with a 150 mg/kg ketamine and 15 mg/kg xylazine cocktail (10 μL/g). For all intravenous injections, mice were gently warmed with a heat lamp, placed on a restrainer, tail sterilized with alcohol pads, and the injection was placed into a lateral tail vein. All animal experiments were done in accordance with protocols approved by the Institutional Animal Care and Use Committee of MSKCC and followed National Institutes of Health guidelines for animal welfare.
9
Controlled Environment for Mouse Study
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B6D2F1 mice weighing 25–30 g (Jackson Laboratories; Barr Harbor, ME, USA) were allowed to acclimatize for seven days following arrival at our Comparative Medicine Facility under a controlled temperature (20−22 °C) and humidity (20−40%) environment with a 12-h light—dark cycle. All experimental protocols were approved by the IACUC (Institutional Animal Care and Use Committee) of Loyola University Chicago Health Sciences Division.
10
B6D2F1 Mice Aerosol Infection Study
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Eight-week-old female B6D2F1 mice (The Jackson Laboratory, Bar Harbor, ME, USA) were used in this study. All animal procedures, including aerosol infection, oral gavage treatment, postinfection/treatment monitoring, were performed following standard protocols approved by the Institutional Animal Care and Use Committee (IACUC) of Rutgers University and were in compliance with institutional and governmental guidelines regulating the care and use of laboratory animals in experimental research.
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