Mir 155 ko mice

C57BL/6 wild-type (WT) and miR-155 KO mice were purchased from The
Jackson Laboratory (Bar Harbor, ME).^{(17 (link),18 (link))} Mice were
housed at the University of Massachusetts Medical School (UMMS) animal facility
in compliance with all Institutional Animal Use and Care Committee directives.
For ALD, 8- to 10-week-old WT and miR-155 KO female mice (n = 8–10)
received either a Lieber-DeCarli diet containing 5% (v/v) alcohol or an
isocaloric dextrin maltose diet for 5 weeks.^{(17 (link))} Alcoholic hepatitis (AH) in mice was
induced using the National Institute on Alcohol Abuse and Alcoholism (NIAAA)
model.^{(20 (link))} After
experimental treatments, blood and liver samples were collected, processed
immediately, and stored at −80°C for further analyses.^{(12 (link),17 (link),18 (link))}

Animal studies were approved by the University of Massachusetts Medical School (UMMS) Institutional Animal Use and Care Committee (Worcester, MA). miR-155 KO mice were purchased from Jackson Laboratory (Bar Harbor, Maine, USA) and a breeding colony was maintained in the animal facility of UMMS. Wild type (WT) control mice, C56BL/6 were also obtained from Jackson Laboratory. For chronic ethanol feeding, female mice (n=8–10, 8 weeks old) received 5% (v/v) ethanol (36% ethanol-derived calories) containing Lieber-DeCarli diet or control (pair-fed) diet for 5 weeks. For pair-fed diet, alcohol-derived calories were substituted with dextrin-maltose (Bio-Serv, New Jersey, USA) as described previously [6 (link)]. For CCl₄ treatment, WT or miR-155 KO male mice (n=6, 8–10 weeks old) were treated either with corn oil (vehicle control) or CCl₄ (0.6ml/kg i.p.) for 2 or 9 weeks, as described [15 ]. Mice were sacrificed 72h after last CCl₄ injection. At the end of the experiment, blood was collected by cheek bleeding and mice were sacrificed [6 (link)]. Liver tissue was collected and snap frozen for proteins and in RNA later (Qiagen, Germany) for RNA extraction. All samples were stored at −80°C.