Minispec analyst ad

Body weight was measured weekly using an Ohaus Scout Pro 200g scale with precision to a single decimal place (Nänikon Switzerland). Body composition (fat mass: FM; fat-free mass: FFM, & extracellular fluid) were measured on a mouse carcass-calibrated [21 (link)] time-domain-NMR (Minispec Analyst AD; Bruker Optics, Silberstreifen, Germany).

BW was measured (± 0.1 g) daily in all mice throughout the experiment using an Ohaus Scout Pro 200g scale (Nänikon Switzerland, between 09:00–09:30h). Body composition [fat mass (FM), fat-free mass (FFM), and extracellular fluid] was measured by time-domain-NMR (Minispec Analyst AD; Bruker Optics, Silberstreifen, Germany) [16 (link)] once per week. Food was placed on the top of the cages and food intake was recorded daily for all mice throughout the study. Since mice were housed 3 per cage, food intake data was monitored on a per cage basis. Group housing the Lep^ob/ob mice (3 per cage) reduced stress and the individual mice lost body weight at the same rate. 24 hour core body temperature of mice was measured during the first week of the weight maintenance phase (Fig 1). Rectal core body temperature was measured every 3 h for 24 h using a Thermalert Monitoring Thermometer starting at 1500.