The human chorionic trophoblast cells (HTR8/SVneo) were obtained from Shanghai kanglang biotechnology co., LTD (China). The cells originated from human first trimester placenta and immortalized by transfection with a cDNA construct that encodes the simian virus 40 large T antigen (Graham et al., 1993 ). HTR8/SVneo cells were maintained in RPMI 1,640 solution (Sigma) supplemented with 10% fetal bovine serum (FBS). The medium was supplemented with 100 U/ml penicillin and 100 U/ml streptomycin (Sigma). HTR8/SVneo cells were seeded in 24‐well plates in humidified 5% CO2 at 37°C until reaching 70%–80% confluence.
Htr8 svneo
Manufactured by Merck Group
Sourced in United States
The HTR8/SVneo is a cell line derived from human first-trimester extravillous trophoblast. It is commonly used as an in vitro model for the study of trophoblast biology and placental development.
Automatically generated - may contain errors
Lab products found in correlation
Jeg 3, by American Type Culture Collection (2 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Htr 8 svneo, by Procell (1 mentions)
Dmem f12 gibco, by Thermo Fisher Scientific (1 mentions)
Huvecs, by American Type Culture Collection (1 mentions)
Gelatin, by Merck Group (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Egm 2, by Lonza (1 mentions)
Haoec, by PromoCell (1 mentions)
3 protocols using htr8 svneo
1
Culturing Immortalized Human Trophoblast Cells
2
Gestational Diabetes Mellitus Cell Model
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Human trophoblast cells (HTR-8/SVneo) provided by Procell (Wuhan, China) were cultured in RPMI1640 medium containing 10% FBS at 37°C incubator mixed with 5% CO 2 . Cell model of GDM was constructed by treating HTR-8/SVneo cells with HG (30 mM; Sigma-Aldrich, St. Louis, MO, USA) for 24 h as previously described [16] [17] [18] . Cells in the control group were treated with normal glucose (5.5 mM).
3
Choriocarcinoma Trophoblast Cell Lines Protocol
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The choriocarcinoma trophoblast cell lines, BeWo, Jar, and Jeg‐3, were from American Type Culture Collection (ATCC). The non‐tumor trophoblast HTR‐8/SVneo was a gift from Prof. Charles Graham (Queen's University at Kingston, Canada). Human umbilical vein endothelial cells (HUVECs; originally from ATCC) and aortic endothelial cells (HAoECs; originally from PromoCell GmbH) were provided by Dr. Andriana Margariti and Prof. David Grieve, respectively (Queen's University Belfast, UK). BeWo cells were maintained in DMEM/F12 Gibco (Thermo Fisher) supplemented with 2 mmol/L L‐glutamate. Jar and HTR‐8/SVneo were cultured in RPMI 1640 (Sigma‐Aldrich), and Jeg‐3 was cultured in EMEM (ATCC). Both HUVECs and HAoECs were cultured in EGM‐2 (Lonza) with all vendor‐provided supplements in flasks or plates coated with 0.1% gelatin (Sigma‐Aldrich). All growth media contained 10% fetal calf serum. There was no mycoplasma contamination with the cells.
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