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Apo 8 carotenal

Manufactured by Merck Group
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Sourced in United States, France

Apo-8-carotenal is a chemical compound used as a reference standard in laboratory analysis. It is a naturally-occurring carotenoid derived from plants. Apo-8-carotenal is used to identify and quantify similar compounds in samples through analytical techniques such as chromatography and spectroscopy.

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Lab products found in correlation

Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Pancreatin, by Merck Group (2 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Isopropanol, by Thermo Fisher Scientific (1 mentions) Ammonium acetate, by Thermo Fisher Scientific (1 mentions) Retinyl palmitate, by Merck Group (1 mentions) β carotene, by Merck Group (1 mentions) Retinol, by Merck Group (1 mentions) Fungizone, by Thermo Fisher Scientific (1 mentions) Bile extract, by Merck Group (1 mentions) Lipase, by Merck Group (1 mentions) Hepes buffer, by Merck Group (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Pepsin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Porcine bile extract, by Merck Group (1 mentions) Triolein, by Merck Group (1 mentions) Dmem containing 4.5 g l glucose, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Dutscher (1 mentions)

Close spelling variants of this product

Beta-apo-8′-carotenal β-apo-8′-carotenal E-β-apo-8′-carotenal Trans-β-apo-8′-carotenal

3 protocols using apo 8 carotenal

1

Carotenoid Identification and Quantification

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Methanol, isopropanol, and ammonium acetate were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The calibration standard apo-8-carotenal, β-carotene, retinol, and retinyl palmitate were purchased from Sigma-Aldrich (St. Louis, MO, USA) and β-cryptoxanthin and α-carotene standards were purchased from Santa Cruz Biochemicals (Santa Cruz, CA, USA).
Zhu C., Cai Y., Gertz E.R., La Frano M.R., Burnett D.J, & Burri B.J. (2015). Red palm oil-supplemented and biofortified cassava gari increase the carotenoid and retinyl palmitate concentrations of triacylglycerol-rich plasma in women. Nutrition research (New York, N.Y.), 35(11), 965-974.
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2

Extraction and Quantification of Carotenoids

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Reagents for extraction and chromatographic grade solvents were purchased from Thermo Fisher Scientific (Pittsburgh, PA). Fetal bovine serum (FBS) was purchased from Gemini Bio-Products (West Sacramento, CA). Fungizone, penicillin-streptomycin, L-glutamine and non-essential amino acids were purchased from Life Technologies Corporation (Grand Island, NY). DMEM medium with 25 mmol/L glucose, HEPES buffer, bile extract, porcine amylase, pepsin, lipase and pancreatin, apo-8’-carotenal, all-trans-β-carotene (E-βC; > 98% pure), αC (98% pure) and αTC were purchased from Sigma-Aldrich (St. Louis, MO). Phytoene and phytofluene were kindly provided by Dr. Ken Riedl, Nutrient & Phytochemical Analytic Shared Resource (NPASR) at The Ohio State University. All other reagents and supplies were purchased from ThermoFisher Scientific unless otherwise specified.
Chitchumroonchokchai C., Diretto G., Parisi B., Giuliano G, & Failla M.L. (2017). Potential of golden potatoes to improve vitamin A and vitamin E status in developing countries. PLoS ONE, 12(11), e0187102.
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3

Emulsion Transfer and In Vitro Digestion

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(all-E)-lutein (96%) used for emulsion transfer experiments and in vitro digestions was purchased from CaroteNature (Mussingen, Switzerland). (all-E)-lutein (70% pure) for piglet experiments was from Naturex (Avignon, France). Sunflower oil (Lesieur, France) and canned spinach (Casino, France) were food grade. Sodium acetate and formic acid (HCOOH) were purchased from Merck (Lyon, France). Apo-8'-carotenal (96% pure, used as an internal standard), triolein (65% pure), L-α-phosphatidylcholine and bovine serum albumin (BSA), porcine bile extract and pancreatin were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). DMEM containing 4.5 g/L glucose, trypsin-EDTA (500 mg/L and 200 mg/L, respectively), non-essential amino acids, penicillin/streptomycin and PBS were from Life Technologies (Villebon sur Yvette, France). Foetal bovine serum (FBS) was from Dutscher (Brumath, France). Deionized H 2 O was purified by a Milli-Q Water Purification system (Millipore, MA, USA). All solvents used were HPLC grade from either SDS (Peypin, France) or Merck.
Margier M., Buffière C., Goupy P., Remond D., Halimi C., Caris-Veyrat C., Borel P, & Reboul E. (2018). Opposite Effects of the Spinach Food Matrix on Lutein Bioaccessibility and Intestinal Uptake Lead to Unchanged Bioavailability Compared to Pure Lutein. Molecular nutrition & food research, 62(11).
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