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W6 32 antibody sc 32235

Manufactured by Santa Cruz Biotechnology
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The W6/32 antibody (sc-32235) is a mouse monoclonal antibody that recognizes the human leukocyte antigen (HLA) class I heavy chain. It is a useful tool for the detection and study of HLA class I proteins.

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Lab products found in correlation

Fusion lumos, by Thermo Fisher Scientific (3 mentions) Q exactive plus, by Thermo Fisher Scientific (3 mentions) Q exactive hf, by Thermo Fisher Scientific (3 mentions)

Spelling variants of this product

W6/32 (5 citations) SC-32 (2 citations)

3 protocols using w6 32 antibody sc 32235

1

HLA Immunoprecipitation and Mass Spectrometry

Cited 2 times
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Soluble lysates from up to 50 million single HLA expressing B721.221 cells and up to 0.2 g from tumor samples were immunoprecipitated with W6/32 antibody (sc-32235, Santa Cruz) as described previously4 (link). 10 mM iodoacetamide was added to the lysis buffer to alkylate cysteines for 71 alleles (Supplementary Table 1c; Supplementary Data 2). Peptides of up to three IPs for single HLA expressing samples and up to four IPs for tumor samples were combined, acid eluted either on StageTips or SepPak cartridges34 (link), and analyzed in technical duplicates using high resolution LC-MS/MS on a QExactive Plus (QE+), QExactive HF (QE-HF) or Fusion Lumos (Thermo Scientific). For acquisition parameters see Supplementary Note 2.
Sarkizova S., Klaeger S., Le P.M., Li L.W., Oliveira G., Keshishian H., Hartigan C.R., Zhang W., Braun D.A., Ligon K.L., Bachireddy P., Zervantonakis I.K., Rosenbluth J.M., Ouspenskaia T., Law T., Justesen S., Stevens J., Lane W.J., Eisenhaure T., Zhang G.L., Clauser K.R., Hacohen N., Carr S.A., Wu C.J, & Keskin D.B. (2019). A large peptidome dataset improves HLA class I epitope prediction across most of the human population. Nature biotechnology, 38(2), 199-209.
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2

Profiling HLA Immunopeptidome from Cancer Cells

Cited 1 time
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Soluble lysates from up to 50 million HLA expressing B721.221 cells or 0.1 to 0.2g cancer cells were immunoprecipitated with W6/32 antibody (sc-32235, Santa Cruz) as described previously1 (link),2 (link). 10 mM iodoacetamide was added to the lysis buffer to alkylate cysteines during the lysis and incubation step (3h, 4C) (Supplementary Note 2) for 71 alleles and 10 tumor samples (Supplementary Table 7).
Peptides of up to three IPs were combined, acid eluted either on StageTips or SepPak cartridges14 (link), and analyzed in technical duplicates using high-resolution LC-MS/MS on a QExactive Plus, QExactive HF, or Fusion Lumos mass spectrometer (Thermo Scientific). For acquisition parameters see Supplementary Methods.
de Miranda Santos I.K., Costa C.H., Krieger H., Feitosa M.F., Zurakowski D., Fardin B., Gomes R.B., Weiner D.L., Harn D.A., Ezekowitz R.A, & Epstein J.E. (2001). Mannan-Binding Lectin Enhances Susceptibility to Visceral Leishmaniasis. Infection and Immunity, 69(8), 5212-5215.
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3

HLA Immunoprecipitation and Mass Spectrometry

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Soluble lysates from up to 50 million single HLA expressing B721.221 cells and up to 0.2 g from tumor samples were immunoprecipitated with W6/32 antibody (sc-32235, Santa Cruz) as described previously4 (link). 10 mM iodoacetamide was added to the lysis buffer to alkylate cysteines for 71 alleles (Supplementary Table 1c; Supplementary Data 2). Peptides of up to three IPs for single HLA expressing samples and up to four IPs for tumor samples were combined, acid eluted either on StageTips or SepPak cartridges34 (link), and analyzed in technical duplicates using high resolution LC-MS/MS on a QExactive Plus (QE+), QExactive HF (QE-HF) or Fusion Lumos (Thermo Scientific). For acquisition parameters see Supplementary Note 2.
Sarkizova S., Klaeger S., Le P.M., Li L.W., Oliveira G., Keshishian H., Hartigan C.R., Zhang W., Braun D.A., Ligon K.L., Bachireddy P., Zervantonakis I.K., Rosenbluth J.M., Ouspenskaia T., Law T., Justesen S., Stevens J., Lane W.J., Eisenhaure T., Zhang G.L., Clauser K.R., Hacohen N., Carr S.A., Wu C.J, & Keskin D.B. (2019). A large peptidome dataset improves HLA class I epitope prediction across most of the human population. Nature biotechnology, 38(2), 199-209.
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