Lactic acid bacteria were cultivated routinely in MRS broth (Himedia, India) at 37°C for 24 - 48 hours. Bifidobacteria were anaerobically cultivated in modified MRS broth containing 0.05% L-cysteine and 1 mg l-1 resazurin (Sigma, US) in an injection vial. Escherichia coli TISTR 780 and Staphylococcus aureus TISTR 1466 were obtained from the Microbiological Resources Centre (MIRCEN), Thailand. All patient isolates of Shigella sonnei, S. flexneri, Salmonella enterica subsp. enteric serovar typhimurium SA2093 and S. Paratyphi A were obtained directly from Microbiological Laboratory of Songklanakarind Hospital, Prince of Songkla University, Hat Yai (Songkhla, Thailand). These pathogens were cultivated in Muller Hinton broth (MHB; Himedia, India) at 37°C for 24 hours.
Muller hinton broth mhb
Manufactured by HiMedia
Sourced in India
Muller Hinton broth (MHB) is a microbiological culture medium used for the growth and cultivation of a variety of microorganisms. It provides the necessary nutrients and growth factors to support the proliferation of various bacterial species.
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Lab products found in correlation
2 protocols using muller hinton broth mhb
1
Cultivation of Lactic Acid Bacteria and Pathogens
2
Antimicrobial Efficacy of Castor Oil
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Castor oil (Amman Pharmaceutical industries CO, Jordan), activated charcoal (Research-Lab fine Chem industries, Mumbai, India), loperamide (Daehwa Pharmaceuticals, Republic of Korea), distilled water (Dallul Pharmaceuticals PLC, Addis Ababa, Ethiopia), methanol (Alpha Chemika, Mumbai, India), ciprofloxacin 5 μg/disc (Becton, Dickinson and Company, Sparks, USA), ceftazidime 30 μg/disc (Oxoid Ltd., Basingstoke, England), muller hinton agar (MHA) (HiMedia Laboratories Pvt. Ltd., India), muller hinton broth (MHB) (HiMedia Laboratories Pvt. Ltd., India) and nutrient agar (Micro master lab, India) were used during the study.
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