Fetal bovine serum (04‐001‐1ACS; Biological Industries, Shanghai, China), lentiviral (GenePharma, China), SYBR Green Master Mix (Q121–02‐AA; Vazyme), Lipofectamine 3000 (L3000015; Invitrogen, CA, USA), RNA‐Quick Purification Kit (RN001; Yishan Biotechnology), PrimeScript™ RT Master Mix kit (RR036A; Takara), PE anti‐B7‐H3 Ig (331606; BioLegend, CA, USA), FITC anti‐B7‐H3 Ig (FAB1027F; R&D), B7‐H3 mouse monoclonal antibody (mAb; 66481‐1‐Ig; Proteintech), FN mouse mAb (66042‐1‐Ig; Proteintech, CA, USA), E‐cadherin mouse mAb (14472; Cell Signaling Technology, Boston, MA, USA), N‐cadherin Rabbit mAb (13116; Cell Signaling Technology), matrix metallopeptidase 9 (MMP9) Rabbit mAb (ab76003; Abcam, Milton, Cambridge, UK), phosphoinositide 3‐kinase (PI3K; 4249; Cell Signaling Technology), phosphorylated (p)‐PI3K (17366; Cell Signaling Technology), protein kinase B (AKT; 4691; Cell Signaling Technology), p‐AKT (4060, Cell Signaling Technology), extracellular regulated protein kinase (ERK; 4695; Cell Signaling Technology), p‐ERK (4370; Cell Signaling Technology), p38 (8690; Cell Signaling Technology), p‐p38 (4511; Cell Signaling Technology), protein A + G agarose (P2055; Beyotime), B7‐H3 Ig (14058; Cell Signaling, USA) were used in this study.
E cadherin mouse mab
Manufactured by Cell Signaling Technology
Sourced in United States
E-cadherin mouse mAb is a monoclonal antibody that specifically recognizes the extracellular domain of E-cadherin, a cell-cell adhesion molecule expressed in epithelial cells. This antibody is useful for detecting and studying the expression of E-cadherin in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
N cadherin rabbit mab, by Cell Signaling Technology (4 mentions)
β actin mouse mab, by Cell Signaling Technology (2 mentions)
Primescript rt master mix kit, by Takara Bio (1 mentions)
P erk, by Cell Signaling Technology (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Protein kinase b akt, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Sybr green master mix, by Vazyme (1 mentions)
Phosphorylated pi3k, by Cell Signaling Technology (1 mentions)
Protein a g agarose, by Beyotime (1 mentions)
Slug rabbit mab, by Cell Signaling Technology (1 mentions)
Ab37150, by Abcam (1 mentions)
Snail rabbit polyclonal antibody, by Abcam (1 mentions)
Mmp2 rabbit polyclonal antibody, by Abcam (1 mentions)
Ab32572, by Abcam (1 mentions)
H3k4me3 rabbit mab, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
Phosphatase inhibitor and protease inhibitor, by CWBIO (1 mentions)
5 protocols using e cadherin mouse mab
1
Comprehensive Profiling of B7-H3 Signaling
2
Comprehensive Antibodies for EMT Analysis
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IKBKE rabbit mAb (No.2905 WB 1:1000, IP 1:100), E-cadherin mouse mAb (No.14472 WB 1:1000 IHC 1:100), N-cadherin rabbit mAb (No.13116 WB 1:1000 IHC 1:100), MMP9 rabbit mAb (No.13667 WB 1:1000 IHC 1:100), Slug rabbit mAb (No.9585 WB 1:1000) and YAP1 mouse mAb (No. 12395 WB 1:1000 IHC 1:100 IP 1:100) were obtained from Cell Signaling Technology (USA). β-catenin rabbit polyclonal antibody (ab32572 WB 1:5000 IHC 1:100), Snail rabbit polyclonal antibody (ab180714 WB 1:1000 IHC 1:100), Vimentin rabbit polyclonal antibody (ab45939 WB 1:1000 IHC 1:10000), MMP2 rabbit polyclonal antibody (ab37150 WB 1:1000 IHC 1:100), Twist rabbit polyclonal antibody (ab49254 1:500), TEAD2 rabbit polyclonal antibody (ab83670 WB 1:500) and IKBKE rabbit polyclonal antibody (ab7891 IHC 1:100) were purchased from Abcam (UK). TEAD2 rabbit polyclonal antibody (sc-67115 IP 1:50) were from Santa Cruz (USA). GADPH mouse mAb (TA309157 WB 1:2000) was obtained from ZSGB-BIO (China).
3
Protein Expression Analysis by Western Blot
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H3K4me3 rabbit mAb, E-cadherin mouse mAb, N-cadherin rabbit mAb, and β-actin mouse mAb were purchased from Cell Signaling Technology (Danvers, MA, USA). The cells were lysed in a buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 5 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, and 1% Nonidet P 40 with a mixture of protease inhibitors before Western blot assay.
4
Protein Extraction and Western Blot
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The total protein was extracted using RIPA buffer (Beyotime Institute of Biotechnology, China) mixed with a phosphatase inhibitor and protease inhibitor (CWBIO, China). Equal amounts of protein (20–40 µg) were separated via SDS‒PAGE and transferred onto PVDF membranes (Millipore, MA). After using 5% milk blocking for 1 h, the membranes were placed with antibodies targeting GAPDH monoclonal antibody 1:60000 (#60004-1-lg, Proteintech, China), GLUT3 polyclonal antibody 1:5000 (#20403-1-AP, Proteintech, China), E-Cadherin Mouse mAb 1:1000 (#14,472, Cell Signaling Technology, USA), ZEB1 polyclonal antibody 1:1000 (#21544-1-AP, Proteintech, China), N-cadherin XP Rabbit mAb 1:1000 (#13,116,Cell Signaling Technology, USA), Vimentin XP Rabbit mAb 1:1000 (#5741, Cell Signaling Technology, USA), and NF-κB XP Rabbit mAb 1:1000 (#8242, Cell Signaling Technology, USA) at 4 °C overnight. The membrane was then incubated with the secondary antibodies 1:3000 (Beyotime). The visualisation of antibodies was completed through the ECL system.
5
Western Blot Analysis of EMT Markers
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The cells were lysed using the mammalian protein extraction reagent, RIPA (Beyotime, Shanghai, China). Equivalent amounts (40 µg) of cell protein lysates were electrophoresed on 10% SDS-polyacrylamide gel, transferred to a PVDF membrane. Then the membrane was incubated with primary antibodies with TSPAN7 (1:1,000, Abcam, Massachusetts, USA), N-cadherin (1:2,000, CST, Massachusetts, USA), E-cadherin (1:2,000, CST), vimentin (1:2,000, CST), and tubulin (1:2,000, CST) overnight at 4°C and followed incubation by horseradish peroxidase-labeled secondary antibody. Tubulin was used as a protein-loading control. The immune complexes were tested by chemiluminescence. E-cadherin mouse mAb, N-cadherin rabbit mAb, and β-actin mouse mAb were purchased from Cell Signaling Technology. The cells were lysed in a buffer containing 20 mM Tris–HCl (pH 7.5), 150 mM NaCl, 5 mM EGTA, and 1% NP-40 with a mixture of protease inhibitors before Western blot assay.
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