Human pancreatic cancer cell lines PANC-1, HS766T, AsPC-1 and human normal pancreatic duct epithelial cell lines HPDE6-C7 were all purchased from BeNa Culture Collection, with the cell numbers of BNCC338219, BNCC101687, BNCC100652 and BNCC338285. All cell culture media were composed of 90% DMEM high glucose culture fluid and 10% fetal bovine serum, and the culture conditions were at 37°C, with 5% CO2. The cells were passaged for 2–3 generations. DMEM high glucose medium and fetal bovine serum were all purchased from Thermofisher (China), with the item number of 10569044 and 10099141.
Aspc 1
Manufactured by BNCC
Sourced in China
The AsPC-1 is a cell line derived from a human pancreatic adenocarcinoma. It is commonly used in research as a model for pancreatic cancer studies.
Automatically generated - may contain errors
Lab products found in correlation
Panc 1, by BNCC (5 mentions)
Hpde6 c7, by BNCC (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Hs766t, by BNCC (2 mentions)
Bxpc 3, by BNCC (2 mentions)
Capan 1, by BNCC (2 mentions)
Dmem high glucose medium, by Thermo Fisher Scientific (1 mentions)
Gemcitabine, by Selleck Chemicals (1 mentions)
Sw1990, by BNCC (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Cfpac 1, by BNCC (1 mentions)
Penicillin, by Beyotime (1 mentions)
Streptomycin, by Beyotime (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
5 protocols using aspc 1
1
Culturing Human Pancreatic Cell Lines
2
Gemcitabine Cytotoxicity in PC Cell Lines
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Four human PC cell lines (SW1990, AsPC-1, PANC-1 and BxPC-3) and normal human pancreatic ductal epithelial cell line HPDE6c7 were bought from BeNa Culture collection (Beijing, China) and cultured in RPMI 1640 medium (Gibco, Grand Island, NY, U.S.A.). Culture medium contained 10% fetal bovine serum (Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin–streptomycin (Invitrogen). Cells were allowed to grow in a humidified incubator at 37°C with 5% CO2.
SW1990 and BxPC-3 cell lines (3 × 104 cells/well) were planted in 96-well plates and treated with various concentrations (ranging from 0 to 128 μg/ml) of gemcitabine (Selleck, Houston, TX, U.S.A.) for 72 h.
SW1990 and BxPC-3 cell lines (3 × 104 cells/well) were planted in 96-well plates and treated with various concentrations (ranging from 0 to 128 μg/ml) of gemcitabine (Selleck, Houston, TX, U.S.A.) for 72 h.
3
Culturing Pancreatic Cell Lines
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The cells PANC-1, CFPAC-1, ASPC-1, BXPC-3, CAPAN-1, CAPAN-2 and normal human pancreatic duct epithelial (HPDE6-C7) were purchased from BeNa Culture Collection (Bejing, China). Human embryonic kidney 293T cells were purchased from the Cell Repository of the Chinese Academy of Sciences (Shanghai, China). All cells were cultured in DMEM or RPMI1640 or IMDM supplemented with 10% serum, 100 U/ml penicillin (Beyotime, China), and 100 mg/mL streptomycin (Beyotime, China) at 37 °C with 5% CO2.
4
Pancreatic Cancer Cell Line Cultivation
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PANC-1 and AsPC-1 cells were used due to their clear genetic background and availability. Human pancreatic ductal adenocarcinoma (PAAD) cells: AsPC-1, PANC-1, and HPDE6-C7 (human normal pancreatic epithelial cells) were provided by BeNa Culture Collection (Xinyang, China). HPDE6-C7 and AsPC-1 cells were cultured and maintained in RPMI 1640 medium. DMEM was used for culturing PANC-1 cells.
5
Culturing Pancreatic Cell Lines
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Human pancreatic duct epithelial cell line HPDE6-C7 (BNCC338285) and pancreatic cancer cell lines PANC-1 (BNCC277096), Capan-1 (BNCC337700), AsPC-1 (BNCC277096), and Hs766T (BNCC350780) were all provided with BeNa Culture Collection (Beijing, China). All of them were cultured in DMEM (Gibco, USA) plus 10% fetal bovine serum (FBS; Gibco, USA), supplemented with an appropriate amount of streptomycin (100 mg/mL; Gibco, USA) and penicillin (100 units/mL; Gibco, USA) in a constant temperature incubator at 37 °C and 5% CO2.
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