Albumin concentration was analyzed in tissue culture supernatants from normal, primary biliary cirrhosis (PBC)/primary sclerosing cholangitis (PSC) and alcoholic liver disease (ALD) hepatocytes at days 1, 3, 5 and 7, by means of a sandwich enzyme-linked immunosorbent assay kit (Abnova, Taipei City, Taiwan). Similarly, urea synthesis was confirmed by means of a quantitative colorimetric urea determination method (QuantiChrom Urea Assay Kit, Bioassay Systems, Hayward, CA, USA).
Sandwich enzyme linked immunosorbent assay kit
Manufactured by Abnova
Sourced in United States
The Sandwich enzyme-linked immunosorbent assay (ELISA) kit is a laboratory equipment used for quantitative analysis of target analytes in biological samples. The kit utilizes the sandwich ELISA technique, where a capture antibody is immobilized on a solid support, and the target analyte is sandwiched between the capture antibody and a detection antibody conjugated with an enzyme. The enzyme catalyzes a colorimetric reaction, allowing for the quantification of the target analyte.
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Lab products found in correlation
2 protocols using sandwich enzyme linked immunosorbent assay kit
1
Hepatocyte Albumin and Urea Assays
2
Venous Blood Collection and Processing
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From each participant, 3 mL of venous blood was collected and the samples were divided into two parts: (1) 1.5 mL of venous blood was added into a tube containing EDTA for complete blood count (CBC) determination and (2) 1.5 mL of the remainder blood was allowed to clot for 30 minutes before centrifugation for 15 minutes at 1000g; the serum was removed and assayed immediately or aliquot and stored at −20 °C.
The CBC was performed on coulter counter T890 (Coulter counter, Harpenden, UK). Serum NT-pro BNP was determined using sandwich enzyme-linked immunosorbent assay kit (Abnova, Walnut, CA, USA).
The CBC was performed on coulter counter T890 (Coulter counter, Harpenden, UK). Serum NT-pro BNP was determined using sandwich enzyme-linked immunosorbent assay kit (Abnova, Walnut, CA, USA).
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