Pcmv arms1 pk2 expression vector

The oligonucleotides used in this study were synthesized by Genotech (Daejeon,
Korea). The cloning vector (pGEMT-T easy) was purchased from Promega (Madison,
WI, USA). The pcDNA3 expression vector, pCMV-ARMS1-PK2 expression vector,
Freestyle MAX reagent, FreeStyle CHO-suspension (CHO-S) cells and AssayComplete
medium were purchased from Invitrogen (Carlsbad, CA, USA). The PathHunter
Parental CHO-K1 cell line expressing β-arrestin 2 was
purchased from DiscoveRx (San Diego, CA, USA). The pCORON1000 SP VSV-G-tag
expression vector was purchased from Amersham Biosciences (Piscataway, NJ, USA).
The PMSG-ELISA kit was purchased from DRG International (Mountainside, NJ, USA).
Restriction enzymes and DNA ligation reagents were purchased from Takara Bio
(Shiga, Japan). Fetal bovine serum (FBS) was obtained from HyClone Laboratories
(Logan, UT, USA). The cAMP Dynamic 2 immunoassay kit was purchased from Cisbio
(Codolet, France). CHO-K1 cells and HEK 293 cells were obtained from the Korean
Cell Line Bank (KCLB, Seoul, Korea). The QIAprep-Spin plasmid kit was purchased
from Qiagen (Hilden, Germany). All other reagents were obtained from
Sigma-Aldrich (St. Louis, MO, USA).

The expression vector, pcDNA3, was purchased from Invitrogen (San Diego, CA,
USA). CHO-K1 cells were obtained from the Japanese Cancer Research Resources
Bank (Tokyo, Japan). Endonucleases were purchased from Boehringer Mannheim (MA,
USA) and Takara (Osaka, Japan). Polymerase chain reaction (PCR) reagents were
from Takara (Japan). Ham’s F-12, CHO-S-SFM II, Geneticin, Lipofectamine
2000, and fetal bovine serum (FBS) were obtained from Gibco BRL (MD, USA). The
QIAprep-Spin plasmid kit was purchased from QIAGEN Inc. (Hilden, Germany).
FreeStyle MAX reagent, FreeStyle CHO expression medium, pCMV-ARMS1-PK2
expression vector, anti-myc antibody, antibiotics, and assay complete medium
were purchased from Invitrogen, the PathHunter CHO-K1 β-arrestin Parental
cell line was obtained from DiscoveRx (San Diego, CA, USA) and disposable
spinner flasks were from Corning Incorporated (NY, USA). PMSG ELISA kit was
obtained from DRG International Inc. (Mountainside, NJ). The cAMP Dynamic 2
immunoassay kit was from Cisbio Bioassay (France).
The oligonucleotides were synthesized by Green Gene Bio (Seoul, Korea). Fetal
bovine serum was from Hyclone laboratories (Utah, USA). Centriplus Centrifugal
Filter Devices were purchased from Amicon Bio separations (MA, USA). All other
reagents used were from Sigma-Aldrich (USA) and Wako Pure Chemicals (Osaka,
Japan).