Gtx124246

The cells were lysed using RIPA lysis buffer, and the lysates were collected for Western blotting assay. In brief, equal loading volumes of cell lysates were analyzed by SDS-PAGE, followed by transfer to a PVDF membrane. The membrane samples were probed with antibodies specific for anti-DENV NS2B antibody (1:3000; GeneTex, GTX124246, Inc, Irvine, CA, USA), anti-GAPDH antibody (1:10,000; GeneTex, GTX112827), anti-Nrf2 antibody (1:3000; GeneTex, GTX103322), anti-Lamin B1 (GTX103292), anti-Tubulin (GTX112141), anti-Histone H1 (GTX87506) antibody (1:10,000; GeneTex), and anti-HO-1 antibody (1:3000; Abcam ab13243, Cambridge, MA, USA). The protein abundance of the samples was quantified using ImageJ software following densitometric scanning [20 (link)].

The standard procedure of western blotting was performed as described previously [12 (link)]. The membranes were probed with monoclonal antibodies specific for DENV NS2B, (1:5000; GeneTex, GTX124246), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:10000; GeneTex, GTX100118), Nrf2 (1:3,000; GeneTex, GTX55732), HO-1 (1:3,000; Abcam, ab137749), Keap1 (1:1,000; Abcam, ab196346), and Bach1 (1:1,000; Abcam, ab115210). The ECL detection kit was used for signal detection (PerkinElmer, CT, USA).