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L-leucine is an essential amino acid that serves as a building block for proteins in the body. It is a white, crystalline powder that is odorless and tasteless. L-leucine is commonly used in various laboratory applications, such as cell culture studies and biochemical assays, where it may be required as a nutrient or for specific experimental purposes.

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4 protocols using l leucine

1

Amino Acid and Compound Acquisition Protocol

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L-Alanine (CAS No. 56-41-7), L-Arginine (CAS No. 74-79-3), L-Asparagine (CAS No. 70-47-3), L-Aspartic acid (CAS No. 56-84-8), L-Cysteine (CAS No. 52-90-4), L-Glutamic acid (CAS No. 56-86-0), L-Glutamine (CAS No. 56-85-9), L-Histidine (CAS No. 71-00-1), L-Isoleucine (CAS No. 73-32-5), L-Leucine (CAS No. 61-90-5), L-Lysine (CAS No. 56-87-1), L-Methionine (CAS No. 63-68-3), L-Phenylalanine (CAS No. 63-91-2), L-Proline (CAS No. 147-87-3), L-Serine (CAS No. 56-45-1), L-Threonine (CAS No. 72-19-5), L-Tryptophan (CAS No. 73-22-3), L-Tyrosine (CAS No. 60-18-4), L-Valine (CAS No. 72-18-4), and Glycine (CAS No. 56-40-6) were purchased from MACKLIN. MHY1485 (CAS No. 326914-06-1) and Rapamycin (CAS No. 53123-88-9) were purchased from APExBIO.
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2

Sarafloxacin Hydrochloride Antimicrobial Assay

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Sarafloxacin hydrochloride was purchased from the China Institute of Veterinary Drug Control (Beijing, China). L-leucine and thiourea were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Mueller Hinton (MH) broth, tryptic soy agar (TSA), Luria-Bertani (LB) broth, MacConkey agar, and MH agar were purchased from Guangdong Huankai Microbial Sci & Tech Co., Ltd. (Guangdong, China). M9 Minimal medium was purchased from Shanghai ELITE Biotech Co., Ltd. (Shanghai, China). Methanol and acetonitrile (high-performance liquid chromatography grade) were purchased from Thermo Fisher Scientific (Waltham, MA, United States).
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3

Zein-based Biomaterial Development

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Zein (purity 91%), L-leucine, thiobarbituric acid, and sodium hydroxide were purchased from Macklin (Shanghai, China). Ethylene glycol diglycidyl ether (EGDE), PEI-EDA (average molecular weight ~ 800), phthalaldehyde, 2-mercaptoEthanol, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), guaiacol, hydrogen peroxide (30%), riboflavin, disodium ethylenediaminetetraacetate (EDTA-Na2), D-methionine, nitroblue tetrazolium (NBT) (95%), sodium dihydrogen phosphate dihydrate, disodium hydrogen phosphate heptahydrate, sodium dodecyl sulfonate (electrophoresis level), and sodium tetraborate decahydrate were all obtained from Aladdin (Shanghai, China). Potassium bromide was supplied by Guangzhou Chemical Reagent Factory (Guangzhou, China). Standard protein (Marker), electrophoresis buffer, Coomassie brilliant blue fast staining solution, and 30% acrylamide were all procured from Solaibio (Beijing China). Ethanol, trichloroacetic acid, salicylic acid, and concentrated hydrochloric acid were provided by Tianjin Damao Chemical Reagent Factory (Tianjin, China). All chemicals were analytically pure, except for specific reagents, and they were used without further purification.
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4

Antimicrobial Activity Evaluation

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Netilmicin Sulfate was obtained from Beierka Biopharmaceutical Co., Ltd. (Wuhan, China). l-leucine was purchased from Macklin Inc. (Shanghai, China). Trifluoroacetic acid was obtained from Shanghai Aladdin Reagent Co., Ltd. (Shanghai, China). Methanol was supplied by Honeywell Burdick & Jackson Inc. (Morris, NJ, USA). Pseudomonas aeruginosa (ATCC9027), Escherichia coli (E. coli, ATCC9739), and Staphylococcus aureus (ATCC6538) were purchased from Guangdong Microbiological Detection Center (Guangdong, China). Mueller–Hinton broth medium was supplied by Thermo Fisher Oxoid (Basingstoke, UK). All other reagents were used as received.
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