The organic acids and carbohydrates profiles of the tested fruit juices were determined using high performance liquid chromatography (HPLC), according to the method described by Gutarowska and Czyżowska (2009) [18 (link)]. In addition, the polyphenolic compounds were also characterized using HPLC-DAD method with a diode array detector (Finnigan Surveyor-PDA Plus detector) and a ChromQuest 5.0 chromatography software (Thermo Fisher Scientific Inc., Waltham, MA, USA) as well as using liquid chromatography mass spectrometry (LC-MS; LTQ Velos MS, Thermo Fisher Scientific) following the method described by Antolak et al. (2015) [19 (link)].
Ltq velos ms
Manufactured by Thermo Fisher Scientific
Sourced in United States
The LTQ Velos MS is a high-performance mass spectrometry instrument designed for advanced analytical applications. It features high sensitivity, resolution, and mass accuracy, enabling reliable identification and quantification of a wide range of analytes. The core function of the LTQ Velos MS is to provide accurate and precise mass spectrometric data for scientific research and industrial applications.
Automatically generated - may contain errors
3 protocols using ltq velos ms
1
Profiling Fruit Juice Organic Acids, Carbohydrates, and Polyphenols
2
Cardiolipin Analysis by HPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CL analysis was performed as described in Ref. [51 (link)]. Phospholipid extracts were dissolved in HPLC starting condition and subjected to HPLC-MS/MS analysis. Separation was achieved by reversed-phase HPLC with an Agilent Poroshell 120 EC-C8 2.7 μm 2.1 × 100mm column (Agilent Technologies, Santa Clara, CA, USA) on a Dionex Ultimate 3000 HPLC (Thermo Fisher Scientific, Waltham, MA, USA, 50 °C column oven, 0.4 mL/min flow) with running solvent A (60/40 Acetonitrile/H2O, 10 mM ammonium formate, 0.2% formic acid) and running solvent B (90/10 Isopropanol/Acetonitrile, 10 mM ammonium formate, 0.2% formic acid). Analytes were measured using a LTQ Velos MS (Thermo Fisher Scientific, Waltham, MA, USA) operated in negative ESI mode (3.8 kV, 275 °C capillary temperature, 460–1650 m/z) and data-dependent MS2 acquisition. Thermo raw data was converted to open-source MZML format and Peaks were integrated in MZmine2 [52 ]. Identification was based on a combination of accurate mass, (relative) retention times, and fragmentation spectra, compared to a library of standards. Data was corrected for internal standard, normalized on protein content, quantified using an external dilution series (CL(14:0)4, CL(18:1)4) and further analysed by an in-house pipeline in R. Significance was calculated using a one-way ANOVA with post hoc Tukey correction.
3
Lipidomic Profiling by LC-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The PC/PE analysis was performed as described in 33 (link) , with the modifications described in 13 (link) . 10 µl of the samples were injected by a Dionex Ultimate 3000 HPLC (Thermofisher Scientific Inc, Waltham, USA) with a flowrate of 0.4 ml/min and a column oven temperature of 50 °C. The HPLC was operated in reversed phase mode using an Agilent Poroshell 120 EC-C8 2.7mm 2.1x100mm column (Agilent Technologies, Santa Clara, USA). For gradient elution running Solvent A (4/6 H2O/ammonium format, 0.2% formic acid) and running Solvent B (9/1 isopropanol/acetonitrile, 10 mM ammonium formate, 0.2% formic acid) were used. First, from 0 to 2 minutes, an isocratic flow with 54% Solvent A was applied, followed by a linear decrease from 54% to 28% Solvent A from minute 2 to minute 22. Afterwards the column was washed and again equilibrated at a starting condition of 54% Solvent A. Further, lipids were ionized through negative electrospray ionization (275 °C capillary temperature, with mass range of 460-1650 m/z) in a LTQ Velos MS (Thermo Fisher Scientific Inc., Waltham, USA), whereas MS2 settings were variable depending on the expected PL. For general LC-MS parameters see Supplemental Table 2 and3.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!