Fasting venous blood was collected by venepuncture for the preparation of serum and plasma (Vacutainer; Greiner Bio-One BV). All blood samples were drawn between 08·00 and 11·00 hours by experienced research nurses. For plasma preparation, blood (10 ml) was collected in citrate tubes, centrifuged at 3000 g for 15 min, and plasma was divided into aliquots and stored at −80°C until analysis. For serum preparation, blood (10 ml) was allowed to clot for 30 min at room temperature, centrifuged, and the serum was stored at −80°C.
Vacutainer
Manufactured by Greiner
Sourced in Austria
Vacutainer is a versatile blood collection system used for the collection, transportation, and storage of blood samples. It consists of a sterile, evacuated glass or plastic tube with a closure to maintain the vacuum and prevent contamination.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using vacutainer
1
Blood Sampling for Serum and Plasma Analysis
2
Piglet Gut Microbiome and Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the day 28, one piglet from each replicate pen close to the median body weight was selected for sampling. Blood (7 mL) was collected via jugular venipuncture using vacutainer without anticoagulant (Greiner Bio-One GmbH, Kremsmunster, Austria) [17] , which was subsequently centrifuged at 3,000 ×g for 15 min for serum preparation and stored at -80 ℃ until further analysis.
Three piglets per treatment group were randomly selected for slaughter. The selected piglets were from the different pens and their body weights were close to the median body weight [14] . Approximately 10 g digesta from the mid cecum and colon of each piglet were collected in sterile tubes, ash frozen in liquid nitrogen and stored at -80 ℃ until further analysis [13] . One aliquot of digesta samples were obtained for microbial composition analysis and additional subsamples were taken to determine the short chain fatty acids (SCFAs) in the gut. Intestinal tissues (3.0 cm) were respectively taken from jejunum and ileum, washed with normal saline to remove gut contents, immediately preserved in liquid nitrogen and kept at -80 ℃ for anti-in ammatory analysis.
Three piglets per treatment group were randomly selected for slaughter. The selected piglets were from the different pens and their body weights were close to the median body weight [14] . Approximately 10 g digesta from the mid cecum and colon of each piglet were collected in sterile tubes, ash frozen in liquid nitrogen and stored at -80 ℃ until further analysis [13] . One aliquot of digesta samples were obtained for microbial composition analysis and additional subsamples were taken to determine the short chain fatty acids (SCFAs) in the gut. Intestinal tissues (3.0 cm) were respectively taken from jejunum and ileum, washed with normal saline to remove gut contents, immediately preserved in liquid nitrogen and kept at -80 ℃ for anti-in ammatory analysis.
3
Calf Blood Collection for Veterinary Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The experimental procedures reported herein were conducted with the approval of the Institutional Animal Care and Use Committee of the Freie Universität Berlin. Holstein Friesian calves from 4 commercial dairy herds in Northeast Germany were sampled between August and December 2017. Whole blood was collected from calves (n = 227) 1 to 7 d old by jugular venipuncture using a 20-gauge, 1.5-inch hypodermic needle (Vacutainer, Greiner Bio-One GmbH, Kremesmünster, Austria). Blood was collected into 2 sterile plastic Vacutainer tubes. One tube did not contain any anticoagulant (8.5 mL, BD Vacutainer, Becton Dickinson, Franklin Lakes, NJ), and the other contained lithium heparin (9 mL, Greiner Bio-One GmbH). Samples were stored on ice for transportation to the Freie Universität Berlin.
4
Plasma miRNA Analysis in Rheumatoid Arthritis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used the Life Technologies' protocol to analyze eight miRNAs from 62 plasma samples. 20 healthy human controls aged between 30 and 50 years, 14 healthy non medicated centenarians exceeding 100 years, 28 patients with rheumatoid arthritis aged between 30 and 60 years, were analysed.
The patients with rheumatoid arthritis were in treatment with glucocorticoids and in the remission phase of the pathology. The drug used was Prednisone, a synthetic drug similar to cortisone, used to relieve rheumatic and allergic conditions and to treat leukemia. The dosage was 2,5-5 mg of prednisone oral administration. The donors were volunteers recruited from the longevity AKeA Project 21 (link) (project approved by the Institutional Local Ethics Committee,Azienda Sanitaria Locale n°1 di Sassari, Italy,N° 398/L.) and ubjects signed a written consent prior to blood sampling. Blood samples were collected early in the morning (to reduce the biological variability) by venipucture into a vacutainer (Greiner Bio-One, Monroe, NC, USA) containing K2 EDTA as an anticoagulant. Then we proceeded to the separation of the plasma by centrifugation at 2500 g for 15 min at room temperature. The supernatant containing the plasma was divided into a defined number of aliquots of 200 µL each and stored at -80°C until RNA extraction under a temperature monitoring system.
The patients with rheumatoid arthritis were in treatment with glucocorticoids and in the remission phase of the pathology. The drug used was Prednisone, a synthetic drug similar to cortisone, used to relieve rheumatic and allergic conditions and to treat leukemia. The dosage was 2,5-5 mg of prednisone oral administration. The donors were volunteers recruited from the longevity AKeA Project 21 (link) (project approved by the Institutional Local Ethics Committee,Azienda Sanitaria Locale n°1 di Sassari, Italy,N° 398/L.) and ubjects signed a written consent prior to blood sampling. Blood samples were collected early in the morning (to reduce the biological variability) by venipucture into a vacutainer (Greiner Bio-One, Monroe, NC, USA) containing K2 EDTA as an anticoagulant. Then we proceeded to the separation of the plasma by centrifugation at 2500 g for 15 min at room temperature. The supernatant containing the plasma was divided into a defined number of aliquots of 200 µL each and stored at -80°C until RNA extraction under a temperature monitoring system.
5
Blood Sample Collection and Storage
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were obtained from the cubital vein using the vacutainer (Greiner bio-one, Kremsmünster, Austria). All blood samples were centrifuged at 800 × g for 15 min, aliquoted, and stored frozen at −70°C until use.
6
Piglet Gut Microbiome and Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
On the day 28, one piglet from each replicate pen close to the median body weight was selected for sampling. Blood (7 mL) was collected via jugular venipuncture using vacutainer without anticoagulant (Greiner Bio-One GmbH, Kremsmunster, Austria) [17] , which was subsequently centrifuged at 3,000 ×g for 15 min for serum preparation and stored at -80 ℃ until further analysis.
Three piglets per treatment group were randomly selected for slaughter. The selected piglets were from the different pens and their body weights were close to the median body weight [14] . Approximately 10 g digesta from the mid cecum and colon of each piglet were collected in sterile tubes, ash frozen in liquid nitrogen and stored at -80 ℃ until further analysis [13] . One aliquot of digesta samples were obtained for microbial composition analysis and additional subsamples were taken to determine the short chain fatty acids (SCFAs) in the gut. Intestinal tissues (3.0 cm) were respectively taken from jejunum and ileum, washed with normal saline to remove gut contents, immediately preserved in liquid nitrogen and kept at -80 ℃ for anti-in ammatory analysis.
Three piglets per treatment group were randomly selected for slaughter. The selected piglets were from the different pens and their body weights were close to the median body weight [14] . Approximately 10 g digesta from the mid cecum and colon of each piglet were collected in sterile tubes, ash frozen in liquid nitrogen and stored at -80 ℃ until further analysis [13] . One aliquot of digesta samples were obtained for microbial composition analysis and additional subsamples were taken to determine the short chain fatty acids (SCFAs) in the gut. Intestinal tissues (3.0 cm) were respectively taken from jejunum and ileum, washed with normal saline to remove gut contents, immediately preserved in liquid nitrogen and kept at -80 ℃ for anti-in ammatory analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!