After the animals were sacrificed, the femoral bones were removed and fixed in formalin-buffered (4%) PBS for 24 hours, after which the femurs were decalcified in 10% nitric acid for 3 days. The bone samples were dehydrated through a graded series of ethanol (from 70% to 100%) and diaphanized in xylene. To obtain a distinct view of the defect, the orientation and alignment of the femurs were carefully considered during paraffin embedding. Longitudinal serial sections were prepared at a thickness of 6 μm and mounted on histological slides. Hematoxylin and eosin (H&E) staining was used for general histological observations. The sections were evaluated using a Leica DMLB 80 microscope and a Leica DC300FX camera (Bannockburn, IL, USA). The images were analyzed using Qwin software (version 3 for Windows).
Dmlb 80 microscope
Manufactured by Leica
Sourced in United States
The DMLB 80 is a microscope designed for laboratory use. It features a magnification range and optical configuration suitable for various microscopy applications. The instrument's core function is to enable the observation and analysis of samples at the microscopic level.
Automatically generated - may contain errors
Lab products found in correlation
Dc 300fx camera, by Leica (1 mentions)
Dc 300 fx, by Leica (1 mentions)
Qwin software, by Leica (1 mentions)
Paraplast, by Merck Group (1 mentions)
3 3 diaminobenzidine (dab), by Merck Group (1 mentions)
Ab92298, by Abcam (1 mentions)
Peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
4 protocols using dmlb 80 microscope
1
Histological Analysis of Femoral Bone
2
VP Lobe Histomorphometric Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
At PND 540, samples of VP lobes from the CTR and GLLP groups (n = 12/group) were fixed in Methacarn [78 (link)], diaphanized in xylene, and embedded in Paraplast (Sigma, St. Louis, MO, USA). For the general morphological study, histological slices (5 µm) were stained with Hematoxylin/Eosin. The slides were evaluated using the image analyzer Leica Q-win software and a Leica DMLB 80 microscope connected to a Leica DC300FX (Version 3 for Windows).
3
Immunohistochemical Analysis of Calreticulin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Histological sections of 5 μm (n = 6 per group) were processed as described by Santos et al. [16 (link)]. After the initial steps, the slides were boiled for 30 min in 10 mM sodium citrate solution (pH 6.0) for antigen retrieval. Prostatic sections were blocked in 5% nonfat milk diluted in phosphate-buffered saline (PBS) and incubated with anti-Calreticulin antibody (ab2908) specific primary antibody overnight at 4°C. Slides were washed in PBS and incubated for one hour at room temperature in horseradish peroxidase (HRP)-conjugated secondary antibody. The slides were washed, and the reaction was developed using 3,3′-Diaminobenzidine (DAB, Sigma) and counterstained with hematoxylin for 30 seconds. The reactions were analyzed using a Leica DMLB 80 microscope.
4
SRXN1 Protein Expression in Prostate Cancer Mouse Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Paraffin blocks of all prostatic lobes containing WT and tumor samples (PIN, MT, and AT) from GEMMs were obtained by donation from David Neal's Uro-Oncology Group at the CRUK Cambridge Institute (University of Cambridge, UK). Histological sections (5 μm) of WT and tumor-bearing prostate lobes from the GEMMs (n = 5 per group) were deparaffinized, rehydrated, boiled for 30 min in 10 mM sodium citrate solution (pH 6.0) for antigen retrieval, and quenched in 3% H2O2 methanol solution. Prostate sections were blocked in 5% nonfat milk in phosphate-buffered saline (PBS) and incubated overnight at 4°C with a specific primary antibody against SRXN1 (Abcam, ab92298, 1 : 100), our chosen gene. Next, sections were incubated with a secondary peroxidase-conjugated antibody (Santa Cruz Biotechnology, 1 : 200), which was developed using diaminobenzidine (Sigma-Aldrich) as the chromogen. Slides were counterstained with Harris's hematoxylin. The negative control was obtained by excluding the primary antibody incubation step. The sections were visualized using a Leica DMLB 80 microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!