Aortic arch samples fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned into consecutive 8-μm thick free-floating sections were de-waxed, hydrated, and then repaired using sodium citrate-EDTA. Sections were blocked with goat serum for 1 h at 37 °C and then reacted with mouse anti-8-hydroxy-2’-deoxyguanosine (8-OHdG) antibody (1:500; StressMarq Biosciences, Victoria, BC, Canada) at 4 °C overnight. The sections were washed with PBS and reacted with fluorescein isothiocyanate (FITC) -conjugated goat anti-mouse secondary antibodies (1:100) for 1 h at 25 °C. Antifade mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA) was used to dye the nuclei and for mounting. Images were obtained using a fluorescence microscope (Olympus, Tokyo, Japan). Positive cells were counted using Image Pro Plus 6.0 software.
8 ohdg antibody
Manufactured by StressMarq Biosciences
Sourced in Canada
The 8-OHdG antibody is a laboratory reagent used for the detection and quantification of 8-hydroxy-2'-deoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage. This antibody can be utilized in various analytical techniques, such as ELISA, immunohistochemistry, and Western blotting, to measure the levels of 8-OHdG in biological samples.
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Lab products found in correlation
2 protocols using 8 ohdg antibody
1
Quantifying Oxidative DNA Damage in Aortic Arch
2
Quantifying Oxidative DNA Damage via 8-OHdG ELISA
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Level of 8-OHdG was detected using a ELISA assay 37 (link). For detection of 8-OHdG-modified nucleotides, 250 ng NETs-DNA was added to poly-l-lysine-coated plates and incubated at 4°C overnight, washed twice and blocked with 1% BSA for 2 hours at room temperature. Oxidized nucleotides were detected by 8-OHdG antibody (1:100, StressMarq Biosciences) at room temperature, followed by incubation with horseradish peroxidase (HRP)-conjugated streptavidin. The assay was developed with TMB (3,3′,5,5′-tetramethylbenzidine), and absorbance was analyzed at 450 nm. Serum 8-OHdG was detected in similar procedure.
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