Morphological examination was performed with SEM. HCC cells were cultured at 2 × 105 cells per well in 6‐well plates, after which peptides (100 μg/ml) were added into the wells for another 48 h. The samples were fixed with 3% glutaraldehyde for more than 24 h and washed twice with distilled water. The samples were dehydrated using an acetonitrile gradient and dried under vacuum. Next, samples were mounted on aluminium stubs, after which the samples were sputter‐coated with gold using a sputter coater (Agar Scientific, UK) to prevent beam charging effects. SEM analysis was performed using a Quanta 450 FEG SEM (FEI, USA).
Quanta 450 feg sem
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Quanta 450 FEG SEM is a scanning electron microscope (SEM) that utilizes a field emission gun (FEG) as the electron source. It is designed to provide high-resolution imaging and analytical capabilities for a wide range of materials and applications.
Automatically generated - may contain errors
Lab products found in correlation
Escalab 250, by Thermo Fisher Scientific (3 mentions)
Nicolet 6700, by Thermo Fisher Scientific (3 mentions)
Escalab 250xi, by Thermo Fisher Scientific (2 mentions)
Sputter coater, by Agar Scientific (1 mentions)
Dxr2xi, by Thermo Fisher Scientific (1 mentions)
X pert pro mpd, by Philips (1 mentions)
Sc180, by Olympus (1 mentions)
D2 phaser, by Bruker (1 mentions)
Asap 2020, by Micromeritics (1 mentions)
Tecnai t12 tem, by Thermo Fisher Scientific (1 mentions)
Uc6 ultramicrotome, by Thermo Fisher Scientific (1 mentions)
Zetasizer nano, by Malvern Panalytical (1 mentions)
Miniflex 600, by Rigaku (1 mentions)
D8 advance xrd, by Bruker (1 mentions)
Jxa 8230 epma, by JEOL (1 mentions)
Sc7620 sputter coater, by Quorum Technologies (1 mentions)
9 protocols using quanta 450 feg sem
1
SEM Analysis of Peptide-Treated HCC Cells
2
Comprehensive Material Characterization Protocol
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The crystal structure was studied by X-ray diffraction (XRD, X’Pert Pro MPD, Philips, Holland) using Cu Kα as the radiation source under 40 kV and 40 mA. Morphologies were probed by scanning electron microscopy ((SEM, FEI Quanta 450 FEG SEM). X-ray photoelectron spectroscopy (XPS) spectra were recorded on a photoelectron spectrometer (ESCALAB 250, Thermo Scientific, America), where the binding energy (BE) of the elements was calibrated by the BE of C 1s (284.60 eV). The modulus mapping was measured by atomic force microscope (Bruker, DIMENSION ICON) and conducted in the quantitative nano-mechanics mode (QNM). Raman measurement (Dxr-2xi, Thermo Scientific, America) was performed with in situ homemade cells to observe the O-H stretching peak. The pH meter (Brand: REX, Model: PHBJ-261L)) was used to monitor pH changes.
3
Characterization of Zn and MnO2 Morphology
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Scanning electron microscopy (SEM, FEI Quanta 450 FEG SEM) was applied to observe the Zn and MnO2 morphology. A Bruker D2 Phaser with Cu Kα radiation (λ = 0.15418 nm) operating at 30 kV and 10 Ma was adopted to obtain XRD curves. Optical microscopy (OM, Olympus, SC180) was performed with in situ homemade cells to observe the zinc morphology. The specific surface area was measured with N2 adsorption–desorption measurements (Micromeritics, ASAP 2020) using the Brunauer–Emmett–Teller (BET) method.
4
Imaging Human Lung Epithelial Cells
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Human lung epithelial cells were grown to confluence in six-well plates with or without glass coverslips. Cells were fixed with 2.5% glutaraldehyde, 4% paraformaldehyde in 0.1 M cacodylate buffer. For SEM analyses, coverslips were washed with 0.1 M cacodylate buffer, post-fixed with 1% OsO4, dehydrated, and dried overnight with hexamethyl-D-silazane (HMDS). Coverslips were mounted and coated with 6 nm platinum before viewing on an FEI Quanta 450 FEG SEM. For TEM analyses, plates were washed with 0.1 M cacodylate buffer, post-fixed with 1% OsO4, en-bloc stained with 1% uranyl acetate, followed by dehydration and embedding in LX112 (Ladd Scientific). Monolayers were sectioned on a Leica UC6 ultramicrotome, counterstained with uranyl acetate and lead, and viewed on an FEI Tecnai T12 TEM. To ensure scientific rigor and reproducibility, all groups remained blinded until the completion of data analysis.
5
Multimodal Characterization of FeOxNWs
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FeOxNWs samples (i.e. Bac-FeOxNWs, Bac-FeOxNWs-600, Bac-FeOxNWs-800, Bac-FeOxNWs-1000) were characterized using scanning electron microscopy (SEM, FEI Quanta 450 FEG-SEM, USA), energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD, Rigaku MiniFlex 600, Japan), Fourier transform IR (FTIR) spectroscopy (Nicolet 6700 Thermo Fisher, Australia). The particle size and zeta potential were measured using dynamic light scattering (ZetaSizer Nano, Malvern Instruments Ltd., Worcestershire, UK).
6
Characterizing LSG Sensor Surface Morphology
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The surface morphology of the LSG pressure sensor is observed using a Quanta FEG 450 SEM (FEI Inc.). The 2D image of the LSG surface is captured by a white light interference microscope microXAM-1200 (MapVue AE Inc.).
7
Comprehensive Microstructural Analysis of Solidified Silicone Resin
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The surface micromorphology, chemical composition and distribution of elements, and physical phase of solidified silicone resin were detected by QUANTA FEG 450 SEM (FEI, Hillsboro, OR, USA), Axios XRF (PANalytical B.V, Almelo, The Netherlands), JXA-8230 EPMA (JEOL, Akishima, Tokyo, Japan), and D8 Advance XRD (Bruker, Mannheim, Germany), respectively.
8
Antibiotic-Induced Morphological Changes in Klebsiella pneumoniae
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Morphologic changes; An analysis of K. pneumoniae isolate B6 using an SEM revealed morphologic changes on the cell surface after treatment with each antibiotic both alone and in combination.
Overnight cultures (initial inoculum of105–106 CFU/mL) were performed with ERT, MEM, and COL alone as well as in combinations. ERT, MEM and COL corresponding Cmax serum concentrations 150, 40 and 10 μg/mL, respectively, were used and in vitro activity was assessed at 1 h. The tubes were incubated at 37 °C in a shaking water bath for 1 h and then centrifuged at 3220 g for 10 min. The bacterial cells were fixed with 2.5% glutaraldehyde before being washed and resuspended three times in PBS. The bacterial cultures were incubated on polyethylenimine-coated coverslips (22 mm × 22 mm) for 1 h and immersed for a further hour in 2.5% glutaraldehyde in PBS before rinsing in PBS for 10 min, three times. Dehydration was then performed using increasing concentrations of ethanol in water (10, 30, 50, 70, 90 and 100%) for 10 min in each step. The coverslips were air-dried prior to mounting on 25-mm aluminum stubs with double-sided carbon tabs. Silver liquid was applied to the edges of each coverslip, and these were then dried and gold coated in an SC7620 sputter coater (QUORUM TECHNOLOGIES, Ashford Kent, UK). The cells were imaged by using a Quanta FEG 450 SEM (FEI, Hillsboro, OR, USA) [20 (link)].
Overnight cultures (initial inoculum of105–106 CFU/mL) were performed with ERT, MEM, and COL alone as well as in combinations. ERT, MEM and COL corresponding Cmax serum concentrations 150, 40 and 10 μg/mL, respectively, were used and in vitro activity was assessed at 1 h. The tubes were incubated at 37 °C in a shaking water bath for 1 h and then centrifuged at 3220 g for 10 min. The bacterial cells were fixed with 2.5% glutaraldehyde before being washed and resuspended three times in PBS. The bacterial cultures were incubated on polyethylenimine-coated coverslips (22 mm × 22 mm) for 1 h and immersed for a further hour in 2.5% glutaraldehyde in PBS before rinsing in PBS for 10 min, three times. Dehydration was then performed using increasing concentrations of ethanol in water (10, 30, 50, 70, 90 and 100%) for 10 min in each step. The coverslips were air-dried prior to mounting on 25-mm aluminum stubs with double-sided carbon tabs. Silver liquid was applied to the edges of each coverslip, and these were then dried and gold coated in an SC7620 sputter coater (QUORUM TECHNOLOGIES, Ashford Kent, UK). The cells were imaged by using a Quanta FEG 450 SEM (FEI, Hillsboro, OR, USA) [20 (link)].
9
Nanomaterial Surface Analysis Techniques
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The surface morphology is observed by Quanta FEG 450 SEM (FEI Inc.). The Raman spectroscopy is obtained using a laser with wavelength of 514.5 nm (HORIBA Inc.).The XPS is captured by EscaLab 250XI (Thermo Fisher Scientific Inc.).
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