6 carboxyfluorescein fam labeled probes

Manufactured by Thermo Fisher Scientific

6-carboxyfluorescein (FAM)-labeled probes are synthetic oligonucleotides labeled with a fluorescent dye. They are commonly used as reporter molecules in various molecular biology techniques, such as real-time PCR, fluorescence in situ hybridization (FISH), and DNA sequencing.

Automatically generated - may contain errors

Lab products found in correlation

Abi prism 7900ht thermocycler, by Thermo Fisher Scientific (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Vic labeled probe, by Thermo Fisher Scientific (1 mentions) Taqman universal master mix, by Thermo Fisher Scientific (1 mentions) β actin, by Thermo Fisher Scientific (1 mentions)

2 protocols using 6 carboxyfluorescein fam labeled probes

Muscle mRNA Expression Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted from muscle using TRIzol reagent according to the manufacturer's protocol (Life Technologies, Carlsbad, CA). mRNA levels were measured using TaqMan gene expression assays with 6-carboxyfluorescein (FAM)-labeled probes from Applied Biosystems (Life Technologies) (Mm00437457_m1 for Bin1, Mm00514582_m1 for Dnm2, Mm01282275_m1 for Mtm1, Mm01184733_m1 for Mtmr14, Mm01332489_m1 for Myh1, Mm01332564_m1 for Myh2, Mm01332463_m1 for Myh3, Mm01332518_m1 for Myh4, Mm01319006_g1 for Myh7, Mm01329494_m1 for Myh8, Mm01203489_g1 for Myod1, Mm00446195_g1 for Myog, Mm01354484_m1 for Pax7, Mm01729860_m1 for Ptpla, Mm01175211_m1 for Ryr1, Mm00477663_m1 for Smyd1, Mm00660598_m1 for Smyd2, Mm00510201_m1 for Smyd3, Mm00662052_m1 for Smyd4 and Mm00523415_m1 for Smyd5) and custom oligos for Pax3 (Pax3-F: 5′-CCAGAGGGCGAAGCTTACC-3′, Pax3-R: 5′-GTTGATTGGCTCCAGCTTGTTT-3′, Pax3-Probe: 5′-TCTGGTTTAGCAACCGCCGTGCA-3′, Biosearch Technologies, Petaluma, CA). The level of Gapdh mRNA was used for normalization. The PCR was run using an ABI Prism 7900HT thermocycler and SDS2.1 software (Applied Biosystems). Data were analyzed by the comparative ΔΔCT method.

Stewart M.D., Lopez S., Nagandla H., Soibam B., Benham A., Nguyen J., Valenzuela N., Wu H.J., Burns A.R., Rasmussen T.L., Tucker H.O, & Schwartz R.J. (2016). Mouse myofibers lacking the SMYD1 methyltransferase are susceptible to atrophy, internalization of nuclei and myofibrillar disarray. Disease Models & Mechanisms, 9(3), 347-359.

+ Open protocol

+ Expand

Quantitative RT-PCR Analysis of Gene Expression

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA from both eyes and whole brain tissue of P0 pups were extracted with Trizol/chloroform. For qRT-PCR, the cDNAs of all samples were prepared with a Reverse Transcription kit (Invitrogen). We performed qRT-PCR using an Applied Biosystems 7900 with the TaqMan Universal Master Mix and with 6-carboxyfluorescein (FAM)-labeled probes (Applied Biosystems) for each specific gene and a VIC-labeled probe (Applied Biosystems) for the endogenous control, β-actin (4352341E). The specific gene expression was normalized to mouse β-actin (Applied Biosystems) (Kang et al., 2009b (link)).

Delahanty R.J., Zhang Y., Bichell T.J., Shen W., Verdier K., Macdonald R.L., Xu L., Boyd K., Williams J, & Kang J.Q. (2016). Beyond Epilepsy and Autism: Disruption of GABRB3 Causes Ocular Hypopigmentation. Cell reports, 17(12), 3115-3124.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!