He+ ion irradiated nanodiamond particles were donated by Prof. Huan-Cheng Chang, Academia Sinica. The nanodiamonds were annealed in vacuum at 800°C to form NV− centers. Then, the particles were oxidized in air at 450°C for 2 h to remove graphitic carbon on the surface and yield FNDs. HCl, NaOH, NaCl, glycerol and polyethylenimine (Mw: 50,000–100,000) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan) and ethanol was purchased from Nacalai Tesque, Inc (Kyoto, Japan). Milli-Q water was used for all experiments. Dulbecco’s modified Eagle’s medium (DMEM: Sigma-Aldrich Co., MO, USA), with addition of penicillin-streptomycin (Life Technologies Co., NY, USA) and 10% fetal bovine serum (Biowest, MO, USA), was used for cell culture.
Polyethylenimine (pei)
Manufactured by Fujifilm
Sourced in Japan
PEI is a lab equipment product manufactured by Fujifilm. It is a type of polyetherimide material used in various laboratory applications. The core function of PEI is to provide a durable and high-temperature resistant material for use in lab equipment and devices.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Nacalai Tesque (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Hydrochloric acid (hcl), by Fujifilm (1 mentions)
Sodium hydroxide (naoh), by Fujifilm (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
Glycerol, by Fujifilm (1 mentions)
Trisodium citrate dihydrate, by Kanto Chemical (1 mentions)
Silver nitrate agno3, by Fujifilm (1 mentions)
2 propanol, by Kanto Chemical (1 mentions)
Haucl4 3h2o, by Merck Group (1 mentions)
5 protocols using polyethylenimine (pei)
1
Functionalized Nanodiamond Particles for Cell Culture
2
HEK 293 Cell Transfection Protocol
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HEK 293 cells were plated in 4-well coverglass chamber (Iwaki) in a total volume of 1 ml high-glucose DMEM culture medium, containing 10% heat-inactivated FBS, 100 U/ml penicilin and 100 μg/ml streptomycin, and incubated at 37 °C and 5% CO2. For transfection, 1:1 mixture of 0.323 mg/ml polyethylenimine (Wako) and 100 ng/μl DNA with 75 mM NaCl was incubated for 30 min at room temperature, and 25 μl of the mixture was added into 0.5 ml cell culture. Cells were incubated for 1–2 days after transfection.
3
Synthesis and Characterization of PEI
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4
Synthesis of Polymer-Coated Silver Nanoparticles
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Milli-Q-grade water was utilized to prepare all aqueous solutions. Sodium tetrahydroborate (NaBH4, Tokyo Chemical Industry, Japan), trisodium citrate dihydrate (Kanto Chemical, Japan), silver nitrate (AgNO3, Fujifilm Wako Pure Chemical, Japan), sodium hydroxide (NaOH, Kishida Chemical, Japan), PEI (MW: ~ 10,000, Fujifilm Wako Pure Chemical, Japan), PSS(MW: ~ 70,000, Sigma–Aldrich, United States) ammonium solution (NH3, 28%, Kishida Chemical, Japan), hydrogen peroxide solution (H2O2, 30%, Kishida Chemical, Japan), 1,2-dichloroethane (Nacalai Tesque, Japan), PdOEP (Combi-Blocks, United States), DPA (Tokyo Chemical Industry, Japan), TIPS (Sigma-Aldrich, United States), 2-propanol (Kanto Chemical, Japan) were used as received. EO-EPI as a polymer matrix and ExcevalTM as a polyvinyl alcohol derivative were provided by Osaka Soda, Japan and Kuraray, Japan, respectively. All chemicals were used as purchased.
5
Cellulose Nanofiber Preparation from Kraft Pulp
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Cellulose nanofibers were prepared from never-dried softwood bleached kraft pulp according to the modified method of our previous report.25 (link) An aqueous suspension of the pulp (0.2 wt%, 2 L) was treated by a high-pressure water-jet system (Star Burst, HJP-25008, Sugino Machine Co., Ltd, Uozu, Japan) equipped with a counter-collision chamber. The pulp suspension was ejected from a nozzle with a diameter of 0.10 mm at 245 MPa with 100 cycle repetitions. PEI (average molecular weight: 1800 Da) was purchased from Wako Pure Chemical Industries, Ltd., Osaka, Japan. tert-BuOH (>99.0% purity) was obtained from Tokyo Chemical Industry Co., Ltd., Tokyo, Japan. HAuCl4·3H2O (98% purity) was purchased from Sigma-Aldrich Japan, Ltd., Tokyo, Japan. Artificial seawater (Marine Art SF-1) was purchased from Tomita Pharmaceutical Co., Ltd., Tokushima, Japan. All chemicals were used as received without any further purification.
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