P65 sc 372

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The P65 (sc-372) is a protein-specific antibody produced by Santa Cruz Biotechnology. It is designed to detect the expression of the p65 subunit of the NF-kappa-B transcription factor complex. The antibody can be used for applications such as Western blotting and immunohistochemistry, but its detailed applications and intended use are not provided.

Automatically generated - may contain errors

Lab products found in correlation

Mini protean tgx precast protein gel, by Bio-Rad (1 mentions) Immobilon p pvdf membrane, by Merck Group (1 mentions) Amersham ecl mouse igg, by GE Healthcare (1 mentions) Amersham ecl rabbit igg, by GE Healthcare (1 mentions) Criterion xt bis tris protein gel, by Bio-Rad (1 mentions) β actin, by Abcepta (1 mentions) Pierce ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Complete protease inhibitor cocktail, by Roche (1 mentions) Stripping buffer, by Thermo Fisher Scientific (1 mentions) C rel, by Santa Cruz Biotechnology (1 mentions) Phospho kap1, by Fortis Life Sciences (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Prolong gold antifade mountant, by Thermo Fisher Scientific (1 mentions) Phosphor p65, by Cell Signaling Technology (1 mentions) Alexa fluor 488 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Fv1200, by Olympus (1 mentions) Ikkα β sc 7607, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Sc-372 (69 citations) NF-κB p65 (sc-372) (8 citations) Anti-NFκB p65 (sc-372), (6 citations) Anti-p65 (sc-372, (5 citations) Rabbit anti-p65 (sc-372) (3 citations) Anti-NF-κB-p65 (sc-372 or sc8008, (2 citations) Anti-NFκB-p65 (C-20) sc-372 (2 citations) Anti-NF-kB p65 (C-20: sc-372) (2 citations) Anti-p65 antibody (sc-372), (2 citations)

4 protocols using p65 sc 372

Western Blot Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-cell lysates were prepared in RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Nonidet P40, 0.5% sodium deoxycholate, and 0.1% SDS) supplemented with cOmplete Protease Inhibitor Cocktail (Roche). Proteins were separated by electrophoresis in either 10% Criterion XT Bis-Tris Protein Gels (Bio-Rad) or 4-20% Mini-PROTEAN TGX Precast Protein Gels (Bio-Rad) and the resulting bands were transferred onto Immobilon-P PVDF Membrane (Millipore). All blots were incubated overnight with primary antibodies and developed with the Pierce ECL Western Blotting Substrate (Thermo Scientific). The antibodies used in this study included antibodies (from Cell Signaling Technology) against JNK1 (3708), pc-Jun (2361), c-Jun (9165), pATF-2 (9221), ATF-2 (9226); pIκBα (9246), pp65 (3033), pp38 (9211), p38 (9212), pERK1/2 (4370), ERK1/2 (4695), pSMAD2 (3101), SMAD2 (5339), pSMAD3 (9520), SMAD3 (9523), SMAD4 (38454), Myc (2040); as well as IκBα (610690; BD Biosciences), p65 (sc-372; Santa Cruz Biotechnology), and β-actin (AM1829B; Abgent), and the following secondary antibodies: Amersham ECL Mouse IgG, HRP-linked whole Ab (from sheep) (NA931; GE Healthcare Life Sciences) and Amersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey) (NA934; GE Healthcare Life Sciences).

Li J., Ritelli M., Ma C.S., Rao G., Habib T., Corvilain E., Bougarn S., Cypowyj S., Grodecká L., Lévy R., Béziat V., Shang L., Payne K., Avery D.T., Migaud M., Boucherit S., Boughorbel S., Guennoun A., Chrabieh M., Rapaport F., Bigio B., Itan Y., Boisson B., Fieschi C., Cormier-Daire V., Syx D., Malfait F., Zoppi N., Abel L., Freiberger T., Dietz H.C., Marr N., Tangye S.G., Colombi M., Casanova J.L, & Puel A. (2019). Chronic mucocutaneous candidiasis and connective tissue disorder in humans with impaired JNK1-dependent responses to IL-17A/F and TGF-β. Science immunology, 4(41), eaax7965.

+ Open protocol

+ Expand

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein extracts were separated on SDS–PAGE and were transferred to a PVDF membrane (IPVH00010; Millipore). SuperSignal® West Pico Chemiluminescent substrate (34080; Thermo) was used to visualize the signal. The membranes were reprobed after incubation in stripping buffer (21059; Thermo). Antibodies against the following proteins were used: p65 (sc-372; Santacruz), RelB (4922; Cell signaling), c-Rel (sc-71; Santacruz), Tubulin (T6074; Sigma), Phospho-KAP-1 (A300-767A; Bethyl) and p53 (2524; Cell signalling).

Kim C, & Pasparakis M. (2014). Epidermal p65/NF-κB signalling is essential for skin carcinogenesis. EMBO Molecular Medicine, 6(7), 970-983.

+ Open protocol

+ Expand

Immunofluorescence Analysis of p65 and Phospho-p65

Check if the same lab product or an alternative is used in the 5 most similar protocols

MEFs were seeded on glass coverslips, washed with PBS and fixed in 4% paraformaldehyde. Following fixation, the cells were washed with PBS, permeabilised for 10 min in 0.2% Triton X-100/PBS, blocked with 10% goat serum in PBST (0.1% Tween 20/PBS) for 1 h, and then incubated with a primary antibody, p65 (sc-372; Santa Cruz Biotechnology, Inc.) or phosphor-p65 (#3033; Cell Signaling Technology, Inc.) at 4 °C overnight. After three times wash with PBST, the cells were incubated for 1 h with secondary antibody, Alexa Fluor 488-conjugated anti-rabbit IgG (A11070; Thermo Fisher Scientific Inc., Waltham, MA, USA), and then washed three times with PBST. DNA was counterstained using DAPI (D9542; Merck KGaA). Coverslips were mounted onto slides using ProLong Gold Antifade Mountant (P36934; Thermo Fisher Scientific Inc.). All images were taken by a confocal microscope (FV1200; Olympus Corporation, Tokyo, Japan).

Tanimura A., Nakazato A, & Tanaka N. (2021). MYD88 signals induce tumour-initiating cell generation through the NF-κB-HIF-1α activation cascade. Scientific Reports, 11, 3991.

+ Open protocol

+ Expand

Comprehensive Western Blotting Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was performed as previously described [25 (link)]. Primary antibodies were as follows: pP65 (Ser 536) (sc3020), pP65 (Ser 276) (sc101749), P65 (sc372), COX-2 (cyclooxygenase 2) (sc1747-R), inducible nitric oxide synthase (iNOS) (sc7271), AKT (sc1618), pAKT (Ser 473) (sc7985), inhibitor of kappa B alpha (IKBα) (sc847), pIKBα (Ser32) (sc8404), inhibitor of kappa B kinase alpha/beta (IKKα/β) (sc7607), β-Actin (sc47778), and transcription factor IIB (TFIIB) (sc225) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). pIKKα/β (Ser 176/180) (#2697s) and PTEN and phosphoinositide dependent kinase 1 (PDK1) Ab sample kit (#9652) were purchased from Cell Signaling (Beverly, MA, USA).

Moon K.M., Lee B., Jeong J.W., Kim D.H., Park Y.J., Kim H.R., Park J.Y., Kim M.J., An H.J., Lee E.K., Ha Y.M., Im E., Chun P., Ma J.Y., Cho W.K., Moon H.R, & Chung H.Y. (2017). Thio-barbiturate-derived compounds are novel antioxidants to prevent LPS-induced inflammation in the liver. Oncotarget, 8(53), 91662-91673.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!