Nimodipine

Manufactured by Selleck Chemicals

Nimodipine is a laboratory chemical used for research purposes. It is a dihydropyridine derivative that acts as a calcium channel blocker. Nimodipine is commonly used in scientific research to study calcium signaling pathways and its effects on various cellular processes.

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Lab products found in correlation

3 protocols using nimodipine

Neuroprotective agents against radiation

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A clinical anti-vasoconstriction drug nimodipine and three autophagy inhibitors (Wortamanin, Ly294002 and Chloroquine) (Selleck). These small molecular inhibitors were added directly into fish water. nimodipine (5 μM) was immediately added into fish water after the radiation exposure, and the drug was changed daily for continuing 4 days. Living brain imaging by confocal was done at 2 and 4dpr. For the autophagy inhibitor treat groups, zebrafish embryos were soaked in fish water containing autophagy inhibitors (Wortamanin (1 μM), Ly294002 (10 μM) and Chloroquine (100 μM)) for 6 hours each day, and then washed and changed with fresh water until the death of these radiated larvae.

Ai X., Ye Z., Yao Y., Xiao J., You C., Xu J., Huang X., Zhong J., Fan M., Song X., Shi H., Zhang D, & Zhao C. (2020). Endothelial Autophagy: an Effective Target for Radiation-induced Cerebral Capillary Damage. Scientific Reports, 10, 614.

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Tat Protein Synthesis and Characterization

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Full length HIV Tat (B.FR.83.HXB2; K03455.1 (85 (link))) was prepared on 0.5 mmol 4(hydroxymethyl)phenoxymethyl-copolystyrene-1% divinylbenzene-resin (Applied Biosystems) using an Applied Biosystems 433A Peptide Synthesizer with FastMoc Chemistry as previously described (86 (link)). Peptides were deprotected and cleaved from the support using trifluoroacetic acid supplemented with 10% (vol/vol) (methylsulfanyl)benzene, 5% (vol/vol) H₂O, and 5% (vol/vol) ethane-1,2-dithiol. Crude product was precipitated in 2-methoxy-2-methylpropane at −20°C, washed several times, filtered, and dried under vacuum. Tat was purified by reverse-phase HPLC as previously described (87 (link)). HPLC analysis was performed as previously described (86 (link)), amino acid analysis was performed on a Beckman Model 6300 Amino Acid Analyzer, mass spectrometry was carried out using an Ettan MALDI-TOF mass spectrometer (Amersham Biosciences), and concentration determined with a NanoDrop 1000 spectrophotometer (Thermo Fisher) using a molar attenuation coefficient of 8480 M⁻¹·cm⁻¹ at 280 nm (88 ). Monoclonal anti-Tat antibody was purchased from Abcam (Cat# ab43014, RRID:AB_732970). LPS and EGTA were purchased from Sigma, trypsin was purchased from Gibco, and celecoxib, nimodipine, SC560, and tacrolimus were purchased from Selleck Chemicals.

Kuriyan J., Kong X.P., Krishna T.S., Sweet R.M., Murgolo N.J., Field H., Cerami A, & Henderson G.B. (1991). X-ray structure of trypanothione reductase from Crithidia fasciculata at 2.4-A resolution.. Proceedings of the National Academy of Sciences of the United States of America, 88(19), 8764-8768.

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SARS-CoV-2 Propagation and Drug Screening

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Vero E6 cell line was obtained from American Type Culture Collection and maintained in minimum Eagle’s medium (Gibco Invitrogen) supplemented with 10% fetal bovine serum (FBS; Gibco Invitrogen) and 1% antibiotic/antimycotic (Gibco Invitrogen), at 37 °C in a humidified 5% CO₂ incubator. Huh7 cell line was cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco Invitrogen) supplemented with 10% FBS and 1% antibiotic/antimycotic (Gibco Invitrogen), at 37 °C in a humidified 5% CO₂ incubator.
SARS-CoV-2 (nCoV-2019BetaCoV/Wuhan/WIV04/2019) was propagated in Vero E6 cells^{2 (link)}, and viral titer was determined by 50% tissue culture infective dose (TCID50) as described in our previous study^{5 (link)}. All the infection experiments were performed in a biosafety level-3 (BSL-3) laboratory.
Benidipine HCI (Selleck Chemicals, no. S2017), amlodipine besylate (Selleck Chemicals, S1813), cilnidipine (Selleck Chemicals, S1293), nicardipine HCl (Selleck Chemicals, S4181), nifedipine (Selleck Chemicals, S1808), isradipine (Selleck Chemicals, S1662), nimodipine (Selleck Chemicals, S1747), nisoldipine (Selleck Chemicals, S1748), felodipine (Selleck Chemicals, S1885), 2APB (Selleck Chemicals, S6657), BAPTA-AM (Selleck Chemicals, S7534), and CQ (Sigma-Aldrich, no.C6628) were purchased from indicated companies.

Zhang L.K., Sun Y., Zeng H., Wang Q., Jiang X., Shang W.J., Wu Y., Li S., Zhang Y.L., Hao Z.N., Chen H., Jin R., Liu W., Li H., Peng K, & Xiao G. (2020). Calcium channel blocker amlodipine besylate therapy is associated with reduced case fatality rate of COVID-19 patients with hypertension. Cell Discovery, 6, 96.

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