Anelectron microscope

Manufactured by Zeiss

An electron microscope is a scientific instrument that uses a beam of accelerated electrons to generate high-resolution images of the surface and internal structure of a specimen. The core function of an electron microscope is to provide significantly higher magnification and resolution compared to traditional light microscopes, allowing researchers to study the microstructure and nanoscale features of materials and biological samples.

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Lab products found in correlation

Nile red solution, by Solarbio (1 mentions) 496 diamidino 2 phenylindole dapi, by Yeasen (1 mentions) Oil red o, by Nanjing Jiancheng (1 mentions)

2 protocols using anelectron microscope

Lipid Staining in HepG2 and AML12 Cells

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HepG2 and AML12 cells were first pretreated with FFA as described above. After three
washes using PBS, the cells were stained for 15 min after fixation with 4% formaldehyde
using 0.05 μg/ml Nile red solution (Solarbio). The cell nuclei were stained using 496
diamidino-2-phenylindole (DAPI, Yeasen). The microscopic pictures were captured using an
electron microscope (Zeiss).

Gui W., Zhu Y., Sun S., Zhu W., Tan B., Zhao H., Shang C., Zheng F., Lin X, & Li H. (2021). Knockdown of insulin-like growth factor 2 gene disrupts mitochondrial functions in the liver. Journal of Molecular Cell Biology, 13(8), 543-555.

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Visualizing Lipid Droplet Formation

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HepG2 and AML12 cells were first incubated for 24 h with FFA, washed twice with
phosphate-buffered saline (PBS), fixed for 20 min with 4% formaldehyde, and thereafter
rewashed three times with PBS. The cells were then stained for 15 min using Oil Red O
(Nanjing Jiancheng Bioengineering Institute) and thereafter washed three times with PBS.
The resultant lipid droplets were observed and photographed under an electron microscope
(Zeiss).

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