Fluorescence conjugated streptavidin

Manufactured by Jackson ImmunoResearch

Sourced in United States

Fluorescence‐conjugated streptavidin is a protein that binds strongly to the small molecule biotin. It is commonly used in various immunoassay and cell biology techniques that involve the detection and visualization of biotinylated biomolecules.

Automatically generated - may contain errors

Lab products found in correlation

Cm3050, by Leica (1 mentions) A1 confocal microscope, by Nikon (1 mentions) Rat anti cd31, by BD (1 mentions) Goat anti mmp 9, by R&D Systems (1 mentions) Mouse anti zo 1, by Thermo Fisher Scientific (1 mentions) Novared peroxidase substrate kit, by Vector Laboratories (1 mentions) Rabbit anti mpo, by Abcam (1 mentions) Abc kit, by Vector Laboratories (1 mentions)

2 protocols using fluorescence conjugated streptavidin

Vascular Labeling and Brain Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed as previously described.^[²⁷^] The mice's brains were post‐fixed using 4% paraformaldehyde and dehydrated with gradient sucrose (15%, 20%, and 30%) in PBS. Coronal sections (40 µm thick) were sliced with Leica CM3050S and processed for immunohistochemistry. The brain slices were immersed in PBS twice and incubated in a blocking buffer (PBS containing 0.3% Triton X‐100, 10% horse serum) for 1 h at room temperature. For vascular labeling, the slices were incubated with biotin‐labeled iso‐lectin with a 1:200 dilution (Sigma) followed by fluorescence‐conjugated streptavidin (Jackson Immunoresearch, Pennsylvania, USA) for 1 h at room temperature. Stained sections were mounted onto slides and captured by a Nikon A1 confocal microscope (Nikon, USA). The blood vessel morphological analysis was performed using Angio Tool software.^[²⁸^]

Li S., Hu W., Gong S., Zhang P., Cheng J., Wang S., Wang Y., Shi W., Li Q., Wang F, & Yuan Z. (2023). The Role of PRRC2B in Cerebral Vascular Remodeling Under Acute Hypoxia in Mice. Advanced Science, 10(25), 2300892.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Endothelial, Neutrophil, and Immune Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed on 30μm free-floating sections. Rat anti-CD31 (1:200, BD PharMingen) and rabbit anti-MPO (1:100; Abcam) Abs were used to label endothelial cells and neutrophils, respectively. Other primary Abs includes mouse anti-ZO-1 (1:100, Invitrogen), rabbit anti-PD-1 (1:100, eBioscience), rabbit anti-PD-L1 (1:100, eBioscience), and goat anti-MMP-9 (1:200; R&D Systems). Biotin-conjugated anti-mouse IgG was used to detect extravascular IgG molecules. Biotin was detected with fluorescence-conjugated streptavidin (Jackson ImmunoResearch) or with an ABC kit (Vector) followed by the NovaRED peroxidase substrate kit (Vector) according to the manufacturer's instructions. All images were processed with Image J for cell-based counting of automatically recognized cells. The mean number of cells per mm² was calculated from three fields in the cortex or striatum of each section.

Li P., Mao L., Liu X., Gan Y., Zheng J., Thomson A.W., Gao Y., Chen J, & Hu X. (2014). Essential role of PD-L1 in regulatory T cell-afforded protection against blood-brain barrier damage after stroke. Stroke; a journal of cerebral circulation, 45(3), 857-864.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!