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N acetyl aspartic acid d3

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N-acetyl aspartic acid-d3 is a deuterated version of the amino acid N-acetyl aspartic acid. It is commonly used as an internal standard in analytical methods for the detection and quantification of N-acetyl aspartic acid in various biological samples.

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Lab products found in correlation

15n anthranilic acid, by Merck Group (5 mentions) Testosterone d3, by Merck Group (5 mentions) Gibberellic acid, by Merck Group (5 mentions) Jasmonic acid, by Merck Group (5 mentions) Thymine d4, by Merck Group (5 mentions) Glutamic acid d5, by Merck Group (5 mentions) Sarcosine d3, by Merck Group (4 mentions) Tryptophan 15n2, by Merck Group (4 mentions) Trans zeatin, by Merck Group (3 mentions) Methanol, by Avantor (2 mentions) Chloroform, by Avantor (2 mentions) Ms grade formic acid, by Merck Group (2 mentions) Hplc grade ammonium acetate, by Avantor (2 mentions) Trans zeatine, by Merck Group (2 mentions) Acetonitrile, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions) Trifluroacetic acid, by Agilent Technologies (1 mentions) Hplc grade acetonitrile and water, by Avantor (1 mentions) Formic acid and ammonium acetate, by Merck Group (1 mentions) Acetonitrile, by Avantor (1 mentions)

Close spelling variants of this product

Aspartic acid (75 citations)

5 protocols using n acetyl aspartic acid d3

1

Comprehensive Metabolite Analysis by HPLC-MS

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High-performance liquid chromatography (HPLC)-grade ammonium acetate from Sigma, acetonitrile, methanol, chloroform, and water were procured from Burdick & Jackson (Morristown, NJ). Mass spectrometry-grade formic acid was purchased from Sigma-Aldrich (St Louis, MO).) Metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and Testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).
Amara C.S., Ambati C.R., Vantaku V., Piyarathna D.W., Donepudi S.R., Ravi S.S., Arnold J.M., Putluri V., Chatta G., Guru K.A., Badr H., Terris M.K., Bollag R.J., Sreekumar A., Apolo A.B, & Putluri N. (2019). Serum metabolic profiling identified a distinct metabolic signature in Bladder Cancer Smokers: A key metabolic enzymes associated with patient survival. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 28(4), 770-781.
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2

Metabolite Extraction and Quantification

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HPLC grade ammonium acetate, acetonitrile, methanol, chloroform, ethanol, hexane, and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standard compounds and isotope labeled standards, including N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, 15N anthranilic acid, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).
Ambati C.R., Vantaku V., Donepudi S.R., Amara C.S., Ravi S.S., Mandalapu A., Perla M., Putluri V., Sreekumar A, & Putluri N. (2018). Measurement of methylated metabolites using Liquid Chromatography-Mass Spectrometry and its biological application. Analytical methods : advancing methods and applications, 11(1), 49-57.
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3

Comprehensive Metabolomics Analytical Protocol

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Sources for reagents were: HPLC-grade acetonitrile and water (Burdick & Jackson); mass spectrometry-grade formic acid and ammonium acetate (Sigma-Aldrich); calibration solution containing multiple calibrants in a solution of acetonitrile, trifluroacetic acid, and water (Agilent Technologies); metabolite standards and internal standards, including N-acetyl Aspartic acid-d3, Tryptophan-15N2, Sarcosine-d3, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, 15N Anthranilic acid, and Testosterone-d3 (Sigma-Aldrich).
Amobi-McCloud A., Muthuswamy R., Battaglia S., Yu H., Liu T., Wang J., Putluri V., Singh P.K., Qian F., Huang R.Y., Putluri N., Tsuji T., Lugade A.A., Liu S, & Odunsi K. (2021). IDO1 Expression in Ovarian Cancer Induces PD-1 in T Cells via Aryl Hydrocarbon Receptor Activation. Frontiers in Immunology, 12, 678999.
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4

Comprehensive Metabolite Profiling Approach

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High-performance liquid chromatography (HPLC)-grade ammonium acetate, acetonitrile, methanol, chloroform and water were procured from Burdick & Jackson (Morristown, NJ). MS grade formic acid, standards and internal standards, N-acetyl aspartic acid-d3, tryptophan-15N2, sarcosine-d3, glutamic acid-d5, thymine-d4, gibberellic acid, trans-zeatin, jasmonic acid, anthranilic acid-15N, and testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO). Mixture of LPC 17:0/0:0, PG 17:0/17:0, PE 17:0/17:0, PC 17:0/17:0, TAG 17:0/17:0/17:0, SM 18:1/17:0, MAG 17:0, DAG 16:0/18:1, CE 17:0, ceramide 18:1/17:0, PA 17:0, PI 17:0/20:4, and PS 17:0/17:0. were obtained from Avanti polar lipids (USA) (Supplementary Table S1).
Vantaku V., Dong J., Ambati C.R., Perera D., Donepudi S.R., Amara C.S., Putluri V., Shiva Shankar R., Robertson M.J., Piyarathna D.W., Villanueva M., von Rundstedt F.C., Karanam B., Ballester L.Y., Terris M.K., Bollag R.J., Lerner S.P., Andrea A.B., Villanueva H., Lee M., Sikora A.G., Lotan Y., Sreekumar A., Coarfa C, & Putluri N. (2019). Multi-omics integration analysis robustly predicts high-grade patient survival and identifies CPT1B effect on fatty acid metabolism in Bladder Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(12), 3689-3701.
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5

Metabolomic Profiling of Eosinophilic Esophagitis

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Using mass spectrometry, 48 metabolites were measured and compared between children with active EoE and unaffected controls.
Metabolites and internal standards, including N-acetyl aspartic acid-d3, [15N]2 Tryptophan, Glutamic acid-d5, Thymine-d4, Gibberellic acid, Trans-Zeatine, Jasmonic acid, [15N] Anthranilic acid, and Testosterone-d3, were purchased from Sigma-Aldrich (St. Louis, MO).
Moye L.M., Liu Y., Coarfa C., Putluri N, & Rhoads J.M. (2019). Plasma Urea Cycle Metabolites May Be Useful Biomarkers in Children With Eosinophilic Esophagitis. Frontiers in Pediatrics, 6, 423.
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