Anti type x collagen col 10 antibody

All reagents were from Sigma (Munich, Germany) unless otherwise indicated. Recombinant TGF-β was from R&D Systems (Wiesbaden, Germany). The anti-SOX9 (C-20) antibody was from Santa Cruz Biotechnology (Heidelberg, Germany), the anti-type-II collagen (II-II6B3) antibody from the NIH Hybridoma Bank (University of Iowa, Ames, USA), and the anti-type-X collagen (COL-10) antibody from Sigma. Biotinylated secondary antibodies and ABC were from Vector Laboratories (Grünberg, Germany). Luciferase activity was determined with the Luciferase Assay System (Promega GmbH, Mannheim, Germany) and normalized to total cellular proteins using the BCA protein assay kit (Pierce Thermo Scientific, Fisher Scientific GmbH, Schwerte, Germany). The Cell Proliferation Reagent WST-1 was from Roche Applied Science (Mannheim, Germany). The type-II collagen contents were measured with the native type-II collagen Arthrogen-CIA Capture ELISA kit (Chondrex, Redmond, WA, USA) and those for type-X collagen using a COL-10 ELISA (Antibodies-Online, Aachen, Germany).

All reagents were from Sigma (Munich, Germany) unless otherwise indicated. Recombinant TGF-β3 was from R&D Systems (Wiesbaden, Germany). The anti-SOX9 (C-20) antibody was from Santa Cruz Biotechnology (Heidelberg, Germany), the anti-type-II collagen (II-II6B3) antibody from the NIH Hybridoma Bank (University of Iowa, Ames, USA), and the anti-type-X collagen (COL-10) antibody from Sigma. Biotinylated secondary antibodies and the ABC kit were from Vector Laboratories (Grünberg, Germany). The β-gal Staining Kit and the Cell Proliferation Reagent WST-1 were from Roche Applied Science (Mannheim, Germany). The Beta-Glo^® Assay System was from Promega (Mannheim, Germany). The type-II collagen ELISA (Human COL2A1) was purchased at Cusabio (College Park, MD, USA).

All reagents were from Sigma (Munich, Germany) unless otherwise indicated. Recombinant FGF-2 and TGF-β3 were purchased at Peprotech (Hamburg, Germany). The dimethylmethylene blue dye was from Serva (Heidelberg, Germany). The anti-TGF-β (V) and anti-SOX9 (C-20) antibodies were from Santa Cruz Biotechnology (Heidelberg, Germany), the anti-type II collagen (II-II6B3) antibody from the NIH Hybridoma Bank (University of Iowa, Ames, IA, USA), the anti-type I collagen (AF-5610) antibody from Acris Antibodies (Hiddenhausen, Germany), and the anti-type X collagen (COL-10) antibody from Sigma. Biotinylated secondary antibodies and the ABC reagent were purchased at Vector Laboratories (Alexis Deutschland GmbH, Grünberg, Germany). The TGF-β enzyme-linked immunosorbent assay (hTGF-β1 Quantikine ELISA) was from R&D Systems (Wiesbaden, Germany).