Anti actin c4 monoclonal antibody

Proteins were separated on SDS 5–15% polyacrylamide gels (BIORAD, Hercules, CA) and either stained with Imperial protein stain (Pierce, Rockford, IL) or transferred to 0.45 μm pure nitrocellulose transfer and immobilization membrane (Schleicher and Schuell, Keene, NH). Immunoblotting employed peroxidase conjugated secondary antibodies (Chemicon International, Temecula, CA) and Supersignal West Pico chemiluminescent substrate (Pierce). The anti-actin C4 monoclonal antibody was purchased from Chemicon International. The anti-actin polyclonal antibody was purchased from Cytoskeleton, Inc. The anti-phosphotyrosine 4G10 monoclonal antibody was purchased from Upstate (Millipore). The anti-chlamydial EB polyclonal antibody, the Momp monoclonal antibody and the GAPDH monoclonal antibody were all purchased from Pierce. The anti-c-myc monoclonal antibody was purchased from Genscript (Piscataway, NJ). The anti-chlamydial Hsp60 A57-B9 monoclonal antibody was purchased from Thermo Fisher Scientific (Waltham, MA). Polyclonal rabbit antibodies directed toward C. trachomatis L2 LGV 434 Tarp (CT456) were developed at Rocky Mountain Laboratories as previously described (Clifton et al., 2004 (link)).