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Camptothecin cpt

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Camptothecin (CPT) is a chemical compound that is isolated from the Chinese tree Camptotheca acuminata. It is a topoisomerase I inhibitor that has been used in scientific research as a tool compound.

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Lab products found in correlation

Triton x 100, by Carl Roth (2 mentions) Annexin 5 fitc apoptosis detection kit, by Thermo Fisher Scientific (2 mentions) Anti erk, by Santa Cruz Biotechnology (1 mentions) Anti tyrosine hydroxylase, by Merck Group (1 mentions) 6 hydroxydopamine 6 ohda, by Bio-Techne (1 mentions) Total eif2α, by Cell Signaling Technology (1 mentions) Efaroxan, by Merck Group (1 mentions) Clonidine, by Merck Group (1 mentions) Anti gadd34, by Proteintech (1 mentions) Anti atf4, by Proteintech (1 mentions) Anti cd3 clone okt3, by Thermo Fisher Scientific (1 mentions) Sandimmun, by Novartis (1 mentions)

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Camptothecin (8 citations)

4 protocols using camptothecin cpt

1

Apoptosis and Necrosis Quantification in PBMC

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The level of apoptosis and necrosis of PBMC was determined in three independent experiments using the annexin V-FITC apoptosis detection kit (eBioscience) according to the manufacturer’s instructions. Cells were activated and treated for 24 hrs before they were stained with annexin V and propidium iodide (PI; eBioscience). As control substances, camptothecin (CPT; 30 µg/mL; Tocris) was used for the induction of apoptosis, triton-X 100 (0.5%; Carl Roth) for the induction of necrosis. The amount of apoptosis and necrosis was analysed using flow cytometry.
Falanga C.M., Steinborn C., Muratspahić E., Zimmermann-Klemd A.M., Winker M., Krenn L., Huber R., Gruber C.W, & Gründemann C. (2022). Ipecac root extracts and isolated circular peptides differentially suppress inflammatory immune response characterised by proliferation, activation and degranulation capacity of human lymphocytes in vitro. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 152, 113120.
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2

6-OHDA-Induced Dopaminergic Neurotoxicity

Cited 2 times
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Stock solutions of 6-hydroxydopamine (6-OHDA; Tocris), guanabenz acetate salt, clonidine, efaroxan (Sigma) were prepared in ddH2O; camptothecin (CPT; Tocris) was prepared in DMSO. The following antibodies were used: anti-ATF4 was commercially generated for our laboratory (Liu et al., 2014 (link); Pasini et al., 2015 (link)); anti-GADD34 was from Proteintech; anti-tyrosine hydroxylase (TH) was from EMD Millipore; anti-ERK was from Santa Cruz Biotechnology; anti-parkin (PARK8), anti-phospho-eIF2α (S51) and total eIF-2α were from Cell Signaling Technology.
Sun X., Aimé P., Dai D., Ramalingam N., Crary J.F., Burke R.E., Greene L.A, & Levy O.A. (2018). Guanabenz promotes neuronal survival via enhancement of ATF4 and parkin expression in models of Parkinson disease. Experimental neurology, 303, 95-107.
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3

Apoptosis and Necrosis Quantification in PBMC

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The level of apoptosis and necrosis of PBMC was determined in three independent experiments using the annexin V-FITC apoptosis detection kit (eBioscience) according to the manufacturer’s instructions. Cells were activated and treated for 24 hrs before they were stained with annexin V and propidium iodide (PI; eBioscience). As control substances, camptothecin (CPT; 30 µg/mL; Tocris) was used for the induction of apoptosis, triton-X 100 (0.5%; Carl Roth) for the induction of necrosis. The amount of apoptosis and necrosis was analysed using flow cytometry.
Falanga C.M., Steinborn C., Muratspahić E., Zimmermann-Klemd A.M., Winker M., Krenn L., Huber R., Gruber C.W, & Gründemann C. (2022). Ipecac root extracts and isolated circular peptides differentially suppress inflammatory immune response characterised by proliferation, activation and degranulation capacity of human lymphocytes in vitro. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 152, 113120.
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4

Lymphocyte Activation and Compound Screening

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Lymphocytes were activated with anti-CD3 (clone OKT3) and anti-CD28 (clone 28.2) mAbs (each 100 ng/ml; both from eBioscience, Frankfurt, Germany) in the presence of medium; cyclosporine A (CsA; 4.16 µM; Sandimmun 50 mg/ml, Novartis Pharma, Basel, Switzerland); camptothecin (CPT; 300 µM: Tocris, Bristol, UK); 0.5% Triton-X 100; plant extract; or isolated compounds from A. argyi, as described previously (Zimmermann-Klemd et al., 2019 (link)). After cultivation, the cells were used in biological tests.
Zimmermann-Klemd A.M., Reinhardt J.K., Morath A., Schamel W.W., Steinberger P., Leitner J., Huber R., Hamburger M, & Gründemann C. (2020). Immunosuppressive Activity of Artemisia argyi Extract and Isolated Compounds. Frontiers in Pharmacology, 11, 402.
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